@article{Nocua_Ramírez_Requena_Puerta_2011, title={Maxicircle genome partial sequence of Leishmania braziliensis: assembling and comparison with other trypanosomatids.}, volume={16}, url={https://revistas.javeriana.edu.co/index.php/scientarium/article/view/1348}, DOI={10.11144/javeriana.SC16-1.mgps}, abstractNote={<p><strong>Objective. </strong>With the aim to provide new insights for genotyping and phylogenetic studies of the <em>Leishmania</em> genus, in this study the sequence of the maxicircle in <em>Leishmania braziliensis</em>, strain MHOM-BR-75-M2904, was determined and compared with those reported in other trypanosomatids species. <strong>Materials and methods</strong>. Searches for maxicircle sequences were performed in the unassembled sequences of GeneDB database version 2.1, as well as in the GenBank, using the <em>ND8 </em>and <em>RPS12 </em>genes of <em>L. braziliensis</em> as the initial probes. These sequences were assembled and compared with the homologous sequences of trypanosomatids using the bioinformatics tools LALIGN and ClustalW2. The size of maxicircle was determined by Southern blot assays. <strong>Results</strong>. Two maxicircle fragments of 6535 and 4257 nucleotides were assembled. The sequences of these genes showed high synteny and similarity with the sequences in other <em>Leishmania</em> species. This similarity even was extended to the editing patterns of these molecules.<strong> Conclusions</strong>. Although <em>L. braziliensis</em> is the most divergent species of the<em> Leishmania</em> genus in their nuclear genome, the maxicicircle has a high conservation. This result suggests that the pattern of editing present in the different <em>Leishmania</em> species studied has been conserved also in the subgenus <em>Viannia</em>. These results indicate a high conservation in the editing of mitochondrial transcripts at the genus level.<strong></strong></p> <p><strong>Key words: </strong>kinetoplast DNA (kDNA), RNA editing, <em>Leishmania (Viannia) braziliensis</em>, maxicircle.</p><br />}, number={1}, journal={Universitas Scientiarum}, author={Nocua, Paola and Ramírez, Cesar and Requena, José María and Puerta, Concepción Judith}, year={2011}, month={Jan.}, pages={29–50} }