@article{Quilaguy Ayure_Montoya Solano_Suárez Moreno_Bernal Morales_Montoya Castaño_2010, title={Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strains}, volume={15}, url={https://revistas.javeriana.edu.co/index.php/scientarium/article/view/1375}, DOI={10.11144/javeriana.SC15-1.atdg}, abstractNote={<p><strong>Objective:</strong> to analyze the <em>dhaT </em>gene, one of the genes responsible for the 1,3-propanediol (1,3-PD) production, in two native <em>Clostridium</em> strains. <strong>Materials and methods: </strong>The <em>dhaT</em> gene was amplified by Polimerase Chain Reaction with specific primers designed from <em>Clostridium butyricum</em> VPI1718 operon. Bioinformatics tools like BLASTN, ORF finder, BLASTP and ClustalW were used to determine the identity of the sequence and to assign a function. <strong>Results:</strong> DNA amplification products were obtained from Colombian<em> Clostridium </em>sp. native strains (IBUN 13A and IBUN 158B) and the <em>Clostridium butyricum </em>DSM 2478 strain, which were sequenced. According to the bioinformatics analysis of the above sequences, a high degree of similarity was found with the <em>dhaT </em>gene of different bacterial species. The highest percentage of identity was obtained with the <em>Clostridium butyricum </em>VPI 1718 strain. <strong>Conclusion:</strong> knowledge of the physical structure of the 1,3-PD operon in native strains opens the way for developing genetic and metabolic engineering strategies for improving processes productivity.</p> <p><strong> </strong></p> <p><strong>Key words: </strong>1,3-propanediol, 1,3-propanediol dehydrogenase, <em>dhaT </em>gene, 1,3-propanediol operon.</p><br />}, number={1}, journal={Universitas Scientiarum}, author={Quilaguy Ayure, Diana Milena and Montoya Solano, José David and Suárez Moreno, Zulma Rocío and Bernal Morales, José Mauricio and Montoya Castaño, Dolly}, year={2010}, month={Jan.}, pages={17–26} }