This paper analyzes the extent to which peasants in an area of Guaviare (Colombian Amazon) were subjected to neoliberal ecotourism principles, and examines the socio-political effects related to the incorporation of environmental-business knowledge in their lives. I explore the adaptations and limits of neoliberal environmentality within the framework of ecotourism and the socio-ecological effects of its government technologies on peasants populations. I focus particularly on how these technologies are tailored to the interests of particular groups in the region, and how, through various il/legal mechanisms, ecotourism becomes functional to sophisticated dispossession strategies that peasants must face. On the one hand, I examine dispossessions in the name of conservation by blaming in peasants, and on the other hand, I approach the configuration of other subtler forms of dispossession that I have called politico-ontological dispossessions.