Abstract
The spray-drying technique was applied for the development of three solid formulations of Rhizobium. Sodium alginate and hydroxypropyl methylcellulose (HPMC) with concentrations of 0.5 % were used as polymers. Results showed that none of the solid formulations had negative effects in vitro on the growth-promoting capacities of Rhizobium sp. G58 (p < 0.05). PCA´s first three components explained 84.5 % of the total variance. This analysis concluded that the solid formulation had not negative effects on the biological nitrogen fixation activity in vitro or on the process of nodulation in greenhouse experiments. Symbiosis between Rhizobium and the plant was effective, which suggested that, under controlled conditions, the coating process with the polymers had allowed a controlled release of the bacteria and a proper transfer of Rhizobium sp. from the microparticles to the root of the plant.
Arora NK, Khare E, Maheshwari DK. Plant Growth Promoting Rhizobacteria: Constraints in Bioformulation, Commercialization, and Future Strategies, Plant Growth and Health Promoting Bacteria, Microbiology Monographs 18:97-116, 2011.
doi: 10.1007/978-3-642-13612-2_5
Boza Y, Barbin D, Scamparini ARP. Effect of spray-drying on the quality of encapsulated cells of Beijerinckia sp, Process Biochemistry, 39(10):1275–1284, 2004.
doi: 10.1016/j.procbio.2003.06.002
Champagne CP, Fustier P. Microencapsulation for the improved delivery of bioactive compounds into foods, Current Opinion in Biotechnology, 18(2):184-190, 2007.
doi: 10.1016/j.copbio.2007.03.001
Docampo R, de Souza W, Miranda K, Rohloff P, Moreno SN. Acidocalcisomes—conserved from bacteria to man, Nature Reviews Microbiology, 3(3):251–261, 2005.
doi: 10.1038/nrmicro1097
Glickmann E, Dessaux Y. A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria, Applied Environmental Microbiology, 61(2):793-796, 1995.
Hardy RWF, Holsten RD, Jackson EK, Burns RC. The Acetylene ethylene assay for N2 fixation: laboratory and field evaluation, Plant Physiology, 43(8):1185–1207, 1968.
doi: 10.1104/pp.43.8.1185
Hoagland DR, Arnon DI. The Water-culture method for growing plants without soil, California Agricultural Experimental Station, Circular C347 pp 34, 1950.
Krasaekoopt W, Bhandari B, Deeth H. The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria, International Dairy Journal, 14 (8): 737–743, 2004.
doi: 10.1016/j.idairyj.2004.01.004
Larisch BC, Poncelet D, Champagne CP. Microencapsulation of Lactococcus lactis subsp. Cremoris, Journal Microencapsulation, 11(2): 189–195, 1994.
doi: 10.3109/02652049409040450
Lee BJ, Ryu SG, Cui JH. Controlled release of dual drug-loaded hydroxypropyl methylcellulose matrix tablet using drug-containing polymeric coatings, International Journal of Pharmaceutics, 188(1): 71-80, 1999.
doi: 10.1016/S0378-5173(99)00204-5
Lee KY, Heo TR. Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution, Applied Environmental Microbiology, 66(2): 869-873, 2000
doi: 10.1128/AEM.66.2.869-873.2000
Maciel GM, Chaves KS, Grosso CRF, Gigante ML. Microencapsulation of Lactobacillus acidophilus La-5 by spray-drying using sweet whey and skim milk as encapsulating materials, Journal of Dairy Science, 97(4):1991–1998, 2014.
doi: 10.3168/jds.2013-7463
Mary P, Moschetto N, Tailliez R. Production and survival during storage of spray-dried Bradyrhizobium japonicum cell concentrates, Journal Applied Microbiology, 74(3):340– 344, 1993.
doi: 10.1111/j.1365-2672.1993.tb03035.x
Ozer B, Uzun YS, Kirmaci HA. Effect of microencapsulation on viability of Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum BB-12 during Kasar cheese ripening, International Journal of Dairy Technology, 61(3): 237-244, 2008.
doi: 10.1111/j.1471-0307.2008.00408.x
Park JK, Chang HN. Microencapsulation of microbial cells, Biotechnology Advances, 18(4): 303–319, 2000.
doi: 10.1016/S0734-9750(00)00040-9
Puente ME, Holguin G, Glick BG, Bashan Y. Root-surface colonization of black mangrove seedlings by Azospirillum halopraeferens and Azospirillum brasilense in seawater. FEMS Microbiology Ecology, 29(3):283-292, 1999.
doi: 10.1016/S0168-6496(99)00023-9
Rivera D, Obando M, Barbosa H, Rojas Tapias D, Bonilla Buitrago R. Evaluation of polymers for the rhizobial liquid formulation and their Influence in the Rhizobium-Cowpea Interaction, Universitas Scientiarum, 19(3): 265-275, 2014.
doi: 10.11144/Javeriana.SC19-3.eplr
Serafim LS, Lemos PC, Levantesi C, Tandoi V, Santos H, Reis MA. Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms, Journal of Microbiology Methods, 51(1):1-18, 2002.
doi: 10.1016/S0167-7012(02)00056-8
Vincent J. A manual for the practical study of root nodule bacteria. In International biological programme, vol 15. Blackwell Scientific Publication, Oxford, 1970.
Schoebitz M, López Maria D, Roldán A. Bioencapsulation of microbial inoculants for better soil–plant fertilization. A review, Agronomy for Sustainable Development, 33(4):751-765, 2013.
doi: 10.1007/s13593-013-0142-0
Smit E, Wolters AC, Lee H, Trevors JT, Van Elsas JD. Interaction between a genetically marked Pseudomonas fluorescens strain and bacteriophage øR2f in soil: Effects of nutrients, alginate encapsulation, and the wheat rhizosphere, Microbial Ecology, 31(2):125-140, 1996.
doi: 10.1007/BF00167859
To BCS, Etzel MR. Survival of Brevibacterium linens ATCC 9174 after spray drying, freeze drying, or freezing, Journal of Food Science, 62(1):167-170, 1997b.
doi: 10.1111/j.1365-2621.1997.tb04392.x
Trivedi P, Pandey A. Plant growth promotion abilities and formulation of Bacillus megaterium strain B 388 (MTCC6521) isolated from a temperate Himalayan location Indian, Journal of Microbiology, 48(3):342-347, 2008.
doi: 10.1007/s12088-008-0042-1
Young CC, Rekha PD, Lai WA, Arun AB. Encapsulation of Plant Growth-Promoting Bacteria in Alginate Beads Enriched With Humic Acid, Biotechnology and Bioengineering, 95(1):76- 83, 2006.
doi: 10.1002/bit.20957
Univ. Sci. is registered under a Creative Commons Attribution 4.0 International Public License. Thus, this work may be reproduced, distributed, and publicly shared in digital format, as long as the names of the authors and Pontificia Universidad Javeriana are acknowledged. Others are allowed to quote, adapt, transform, auto-archive, republish, and create based on this material, for any purpose (even commercial ones), provided the authorship is duly acknowledged, a link to the original work is provided, and it is specified if changes have been made. Pontificia Universidad Javeriana does not hold the rights of published works and the authors are solely responsible for the contents of their works; they keep the moral, intellectual, privacy, and publicity rights. Approving the intervention of the work (review, copy-editing, translation, layout) and the following outreach, are granted through an use license and not through an assignment of rights. This means the journal and Pontificia Universidad Javeriana cannot be held responsible for any ethical malpractice by the authors. As a consequence of the protection granted by the use license, the journal is not required to publish recantations or modify information already published, unless the errata stems from the editorial management process. Publishing contents in this journal does not generate royalties for contributors.