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Abstract
La influenza porcina, es una enfermedad respiratoria aguda viral altamente contagiosa, de morbilidad elevada y capaz de provocar complicaciones letales. Tiene gran importancia en la industria pecuaria debido a las cuantiosas pérdidas económicas que ocasiona su alta morbilidad. Por esta razón, en este trabajo se implementaron las pruebas de cultivo celular y RT-PCR para las proteínas M, H y N para la determinación del virus de influenza porcina. En este trabajo, se procesaron y analizaron 82 muestras de hisopos nasales, 12 lavados broncoalveolares y 12 tejidos pulmonares, dando resultados negativos para la detección del virus de influenza. Al comparar la RT-PCR de la proteína M con la de cultivo celular como “Gold Standard” se obtuvo una sensibilidad y especificidad del 100% e índice Kappa de 1, indicando una concordancia muy buena entre las dos pruebas.
Palabras clave: hemaglutinina, influenza, MDCK, neuroaminidasa y RT-PCR.
Abstract
The swine influenza virus, is an acute disease that affects the respiratory tract of the pig, It is highly contagious and have a raised morbility ratio also could turn into a fatal end. This disease has a very important roll into the pig production farms since it is a cause of loses of funds. For that reason, this work implemented the cell culture technique and RT-PCR for M, H and N proteins segments for the fast and accurate diagnosis of this virus. On this work, were tested 82 nasal swabs, 12 broncoalveolars washes, and 12 lung tissues, given negative results for the swine influenza virus. During the compilation of RT-PCR for M protein segment with the cell culture as a gold standard, it attained a sensibility and specificity of 100% and Kappa index of 1 indicating a good concordance between the two probes.
Key words: hemagglutinin, neuroaminidase, influenza, MDCK y RT-PCR
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