Keywords
autotrophic culture
Rubus glaucus Benth
Glomus sp. (GEV02)
in vitro mycorrhization
How to Cite
Abstract
Objective. Obtain an in vitro mycorrhization system in autotrophic culture systems of blackberry plants (Rubus glaucus, Benth). Materials and methods. We used spores and root fragments with vesicles of Arbuscular Mycorrhizal Fungus (AMF) Glomus sp (GEV02). We established an autotrophic culture system of blackberry plantlets comparing two methods of direct inoculation of the AMF. We measured the number of spores produced, the length of the extraradical mycelium as well as the percentage of colonization of the AMF. Additionally, we measured the shoot and root length, and the fresh and dry weight of the leaf and root parts to determine the plant development. Results. The autotrophic culture system was successful for blackberry plants (Rubus glaucus, Benth; an optimal shoot and root growth was observed. Additionally, we obtained a system that allowed the development of Glomus sp. in in vitro conditions, with the formation of structures typical of the symbiosis as well as a good intraradical colonization, with the production of arbuscules and vesicles, development of extraradical mycelium with branched hyphae, and formation of new spores. Conclusion. For the first time, micropropagated blackberry plants associated successfully with an AMF under in vitro conditions, enabling the development of the symbiotic system AMF Glomus sp. associated to roots of micropropagated blackberry plantlets.
Key words: arbuscular mycorrhizal fungi (AMF), autotrophic culture, Rubus glaucus Benth, Glomus sp. (GEV02), in vitro mycorrhization.
Univ. Sci. is registered under a Creative Commons Attribution 4.0 International Public License. Thus, this work may be reproduced, distributed, and publicly shared in digital format, as long as the names of the authors and Pontificia Universidad Javeriana are acknowledged. Others are allowed to quote, adapt, transform, auto-archive, republish, and create based on this material, for any purpose (even commercial ones), provided the authorship is duly acknowledged, a link to the original work is provided, and it is specified if changes have been made. Pontificia Universidad Javeriana does not hold the rights of published works and the authors are solely responsible for the contents of their works; they keep the moral, intellectual, privacy, and publicity rights. Approving the intervention of the work (review, copy-editing, translation, layout) and the following outreach, are granted through an use license and not through an assignment of rights. This means the journal and Pontificia Universidad Javeriana cannot be held responsible for any ethical malpractice by the authors. As a consequence of the protection granted by the use license, the journal is not required to publish recantations or modify information already published, unless the errata stems from the editorial management process. Publishing contents in this journal does not generate royalties for contributors.