The conceptions that nurses have on death shape their meaning, which generates polysemy around this notion. Method: A qualitative study with ethnographic approach whose objective was to explore the meanings that nurses have on cultural and spiritual issues related to the death of patients. 23 interviews with nurses and about 100 hours of observation were performed in a hospital in Medellin (Colombia) between December 2012 and November 2013. Results: The interest in the soul manifested itself as an intention to respect the views of the patient, family and sensitivity to the rituals. The process of dying is part of a culture shock. Conclusion: cultural and religious conceptions are manifested in the practice of care at the end of life. The rituals seek to reassure the patient and family in the transition to the death of the person and also to help the patient “rest in peace”. Therefore, cultural and religious issues relate to an interest in the soul.