Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae)
Published
Apr 2, 2019
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Jaime A. Osorio-Guarín
https://orcid.org/0000-0002-5486-2680
Francy L. García-Arias
https://orcid.org/0000-0003-3112-9950
Roxana Yockteng
https://orcid.org/0000-0003-2133-6139
https://orcid.org/0000-0002-5486-2680
Francy L. García-Arias
https://orcid.org/0000-0003-3112-9950
Roxana Yockteng
https://orcid.org/0000-0003-2133-6139
##plugins.themes.bootstrap3.article.details##
Abstract
Cape gooseberry (Physalis peruviana, L.) is a herbaceous plant belonging to the Solanaceae family that produces an edible berry appreciated for its nutraceutical and pharmaceutical properties. Its production is often limited by diseases and reproducible fruit quality. Recent studies have reported genes associated with fruit quality and resistance response to the root-infecting fungus Fusarium oxysporum f. sp. physali (Foph,) which causes vascular wilt. In order to standardize a method to validate the biological function of candidate genes in the non-model species P. peruviana, we tested the robust approach in reverse genetics, virus induced gene silencing (VIGS). In this study, we validated and optimized VIGS using an insert of the phytoene
desaturase (PDS) gene in a silencing viral vector generated from tobacco rattlevirus (TRV). Leaves infiltrated with Agrobacterium (GV3101 strain) showed photo-bleached segments, which were distinctive for PDS suppression at 7 days post-infection (dpi). More than half of the treated plants showed photo bleaching, indicating an efficiency rate of 50 % of the VIGS protocol. The results of this study showed that VIGS can be used for future functional gene characterization implicated in the immune response, disease resistance and fruit quality in capegooseberry.
Keywords
Physalis peruviana, post-transcriptional gene silencing, Virus Induced Gene Silencing, phytoene desaturase, tobacco rattle virus
References
[1] Jin Z, Mashuta M, Stolowich N, Vaisberg A, Stivers N, Bates P, Lewis W, Hammond G. Physangulidines A, B, and C: Three New Antiproliferative Withanolides from Physalis angulata L., Organic Letters, 14 (5): 1230-1233, 2012.
doi: 10.1021/ol203498a
[2] Ramadan MF. Bioactive phytochemicals, nutritional value, and functional properties of cape gooseberry (Physalis peruviana): An overview, Food Research International, 44 (7): 1830-36, Aug. 2011.
doi: 10.1016/j.foodres.2010.12.042
[3] Ramadan M, El-Ghorab A, Ghanem K. Volatile compounds, antioxidants, and anticancer activities of Cape gooseberry fruit (Physalis peruviana L.): An in-vitro study, Journal of The Arab Society for Medical Research, 10(2): 56, 2015.
doi: 10.4103/1687-4293.175556
[4] Menzel MY. The Cytotaxonomy and Genetics of Physalis, Proceedings of the American Philosophical Society, 95 (2): 132-183, 1951.
https://www.jstor.org/stable/3143331
[5] Popenoe H, King S, León J, Kalinowski L. Goldenberry (Cape Gooseberry), in Lost Crops of the Incas, H. Popenoe, S. King, J. León, and L. Kalinowski, Eds. Washington, USA, National Academy Press, 1989, 415.
[6] Valdenegro M, Almonacid S, Henríquez C, Lutz M, Fuentes L, Simpson R. Effects of drying processes on organoleptic characteristics and the health quality of food ingredients obtained from goldenberry fruits (Physalis peruviana), Open Access Scientific Reports, 2: 642, 2013.
https://www.omicsonline.org/scientific-reports/srep642.php
[7] González C, Barrero LS. Estudio de la marchitez vascular de la uchuva para el mejoramiento genético del cultivo. Bogotá, Colombia, Editorial Kimpress, 2011.
[8] Simbaqueba J, Catanzariti A, González C, Jones D. Evidence for horizontal gene transfer and separation of effector recognition from effector function revealed by analysis of effector genes shared between cape-gooseberry- and tomato-infecting formae speciales of Fusarium oxysporum, Molecular Plant Pathology, 22: 2017.
doi: 10.1111/mpp.12700
[9] Osorio-Guarín JA, Enciso-Rodríguez FE, González C, Fernández-Pozo N, Mueller LA, Barrero LS. Association analysis for disease resistance to Fusarium oxysporum in cape gooseberry (Physalis peruviana L), BMC Genomics, 17 (1): 248, 2016.
doi: 10.1186/s12864-016-2568-7
[10] García-Arias FL, Osorio-Guarín JA, Núñez Zarantes VM. Association Study Reveals Novel Genes Related to Yield and Quality of Fruit in Cape Gooseberry (Physalis peruviana L.), Frontiers in Plant Science, 9:362, 2018.
doi: 10.3389/fpls.2018.00362
[11] Lee WS, Rudd JJ, Kanyuka K. Virus induced gene silencing (VIGS) for functional analysis of wheat genes involved in Zymoseptoria tritici susceptibility and resistance, Fungal Genetics and Biology, 79: 84-88, 2015.
doi: 10.1016/j.fgb.2015.04.006
[12] Burch-Smith TM, Anderson JC, Martin GB, Dinesh-Kumar SP. Applications and advantages of virus-induced gene silencing for gene function studies in plants, The Plant Journal, 39 (5): 734-746, 2004.
doi: 10.1111/j.1365-313X.2004.02158.x
[13] Zhang N, Huo W, Zhang L, Chen F, Cui D. Identification of Winter-Responsive Proteins in Bread Wheat Using Proteomics Analysis and Virus-Induced Gene Silencing (VIGS), Molecular & Cellular Proteomics, 15 (9): 2954-2969, 2016.
doi: 10.1074/mcp.M115.057232
[14] Fantini E, Giuliano G. Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry, in Plant Signal Transduction: Methods and Protocols, J. R. Botella and M. A. Botella, Eds. New York, NY, Springer New York, 65-78, 2016.
doi: 10.1007/978-1-4939-3115-6_7
[15] Groszyk J, Kowalczyk M, Yanushevska Y, Stochmal A, Rakoczy-Trojanowska M, Orczyk W. Identification and VIGS-based characterization of Bx1 ortholog in rye (Secale cereale L.), PLOS ONE, 12 (2): e0171506, 2017.
doi: 10.1371/journal.pone.0171506
[16] Kawalek A, Dmochowska-Boguta M, Nadolska-Orczyk A, Orczyk W. A new BSMV-based vector with modified β molecule allows simultaneous and stable silencing of two genes, Cellular & molecular biology letters, 17 (1): 107-23, 2012.
doi: 10.2478/s11658-011-0041-9
[17] Bennypaul HS, Mutti JS, Rustgi S, Kumar N, Okubara PA, Gill KS. Virus-induced gene silencing (VIGS) of genes expressed in root, leaf, and meiotic tissues of wheat, Functional & Integrative Genomics, 12 (1): 143-156, 2012.
doi: 10.1007/s10142-011-0245-0
[18] Voinnet O. RNA silencing as a plant immune system against viruses, Trends in Genetics, 17 (8): 449-459, 2001.
doi: 10.1016/S0168-9525(01)02367-8
[19] Donaire L, Wang Y, Gonzalez-Ibeas D, Mayer KF, Aranda MA, Llave C. Deep-sequencing of plant viral small RNAs reveals
effective and widespread targeting of viral genomes, Virology, 392
(2): 203-214, 2009.
doi: 10.1016/j.virol.2009.07.005
[20] Ding S-W, Voinnet O. Antiviral Immunity Directed by Small RNAs, Cell, 130 (3): 413-426, 2007.
doi: 10.1016/j.cell.2007.07.039
[21] Baulcombe D. RNA silencing in plants, Nature, 431 (7006): 356-363, Sep. 2004.
doi: 10.1038/nature02874
[22] Lange M, Yellina AL, Orashakova S, Becker A. Virus-Induced Gene Silencing (VIGS) in Plants: An Overview of Target Species and the Virus-Derived Vector Systems, , in Virus-Induced Gene Silencing: Methods and Protocols, A. Becker, Ed. Totowa, NJ, Humana Press, 2013, 1-14.
doi: 10.1007/978-1-62703-278-0_1
[23] Senthil-Kumar M, Mysore KS. Tobacco rattle virus-based virusinduced gene silencing in Nicotiana benthamiana, Nature Protocols, 9 (7): 1549-1562, 2014.
doi: 10.1038/nprot.2014.092
[24] Cai X-Z, Xu Q-F, Wang C-C, Zheng Z. Development of a Virus-Induced Gene-Silencing System for Functional Analysis of the RPS2-Dependent Resistance Signalling Pathways in Arabidopsis, Plant Molecular Biology, 62 (1-2): 223-232, 2006.
doi: 10.1007/s11103-006-9016-z
[25] Ratcliff F, Martin-Hernandez AM, Baulcombe DC. Technical Advance: Tobacco rattle virus as a vector for analysis of gene function by silencing, The Plant Journal, 25 (2): 237-245, 2001.
doi: 10.1046/j.0960-7412.2000.00942.x
[26] Kumagai MH, Donson J, della-Cioppa G, Harvey D, Hanley K, Grill LK. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA, Proceedings of the National Academy of Sciences of the United States of America, 92 (5): 1679-1683, 1995.
[27] Fernandez-Pozo N, Rosli HG, Martin GB, Mueller LA. The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing (VIGS) Constructs for Functional Genomics, Molecular Plant, 8 (3): 486-488, 2015.
doi: 10.1016/j.molp.2014.11.024
[28] Dong Y, Burch-Smith TM, Liu Y, Mamillapalli P, Dinesh-Kumar SP. A Ligation-Independent Cloning Tobacco Rattle Virus Vector for High-Throughput Virus-Induced Gene Silencing Identifies Roles for NbMADS4-1 and -2 in Floral Development, Plant Physiology, 145 (4): 1161-1170, 2007.
doi: 10.1104/pp.107.107391
[29] Liu Y, Schiff M, Marathe R, Dinesh-Kumar SP. Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus, The Plant Journal, 30 (4): 415-429, 2002.
doi: 10.1046/j.1365-313X.2002.01297.x
[30] Liu Y, Schiff M, Dinesh-Kumar SP. Virus-induced gene silencing in tomato, The Plant Journal, 31 (6): 777-786, 2002.
doi: 10.1046/j.1365-313X.2002.01394.x
[31] Liu E, Page JE. Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus, Plant Methods, 4(1): 5, 2008.
doi: 10.1186/1746-4811-4-5
[32] Velásquez AC, Chakravarthy S, Martin GB. Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato, Journal of Visualized Experiments JoVE, (28): 1292, 2009.
doi: 10.3791/1292
[33] Romero I, Tikunov Y, Bovy A. Virus-induced gene silencing in detached tomatoes and biochemical effects of phytoene desaturase gene silencing, Journal of Plant Physiology, 168 (10): 1129-1135, 2011.
doi: 10.1016/j.jplph.2010.12.020
[34] Chung E, Seong E, Kim Y-C, Chung EJ, Oh S-K, Lee S, Park JM, Joung YH, Choi D. A Method of High Frequency Virusinduced Gene Silencing in Chili Pepper (Capsicum annuum L. cv. Bukang), Mol. Cells, 17 (2): 377-380, 2004.
[35] Brigneti G, Martín-Hernández AM, Jin H, Chen J, Baulcombe DC, Baker B, Jones JDG. Virus-induced gene silencing in Solanum species, The Plant Journal, 39 (2): 264-272, 2004.
doi: 10.1111/j.1365-313X.2004.02122.x
[36] Chen J-C, Jiang C-Z, Reid MS. Silencing a prohibitin alters plant development and senescence, The Plant Journal, 44 (1): 16-24, 2005.
doi: 10.1111/j.1365-313X.2005.02505.x
[37] Pang J, Zhu Y, Li Q, Liu J, Tian Y, Liu Y, Wu J. Development of Agrobacterium-Mediated Virus-Induced Gene Silencing and Performance Evaluation of Four Marker Genes in Gossypium barbadense, PLOS ONE, 8 (9): e73211, 2013.
doi: 10.1371/journal.pone.0073211
[38] Meng L-H, Wang R-H, Zhu B-Z, Zhu H-L, Luo Y-B, Fu D-Q. Efficient Virus-Induced Gene Silencing in Solanum rostratum, PLOS ONE, 11 (6): e0156228, 2016.
doi: 10.1371/journal.pone.0156228
[39] Zhang J, Yu D, Zhang Y, Liu K, Xu K, Zhang F, Wang J, Tan G, Nie X, Ji Q, Zhao L, Li C. Vacuum and Co cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize, Frontiers in Plant Science, 8: 1-12, 2017.
doi: 10.3389/fpls.2017.00393
[40] Kirigia D, Runo S, Alakonya A. A virus-induced gene silencing (VIGS) system for functional genomics in the parasitic plant Striga hermonthica, Plant Methods, 10 (1): 16, 2014.
doi: 10.1186/1746-4811-10-16
[41] Yan H, Fu D, Zhu B, Liu H, Shen X, Luo Y. Sprout vacuuminfiltration: a simple and efficient agroinoculation method for virus-induced gene silencing in diverse solanaceous species, Plant Cell Reports, 31 (9): 1713-1722, 2012.
doi: 10.1007/s00299-012-1285-1
[42] Xu H, Xu L, Yang P, Cao Y, Tang Y, He G, Yuan S, Ming J. Tobacco rattle virus-induced Phytoene desaturase (PDS) and Mg-chelatase H subunit (ChlH) gene silencing in Solanum pseudocapsicum L., PeerJ, 6 e4424, 2018.
doi: 10.7717/peerj.4424
[43] Wang T, Wen L, Zhu H. Effectively organ-specific virus induced gene silencing in tomato plant, Journal of Nature and Science (JNSCI), 1 (1): 34, 2015.
doi: 10.1021/ol203498a
[2] Ramadan MF. Bioactive phytochemicals, nutritional value, and functional properties of cape gooseberry (Physalis peruviana): An overview, Food Research International, 44 (7): 1830-36, Aug. 2011.
doi: 10.1016/j.foodres.2010.12.042
[3] Ramadan M, El-Ghorab A, Ghanem K. Volatile compounds, antioxidants, and anticancer activities of Cape gooseberry fruit (Physalis peruviana L.): An in-vitro study, Journal of The Arab Society for Medical Research, 10(2): 56, 2015.
doi: 10.4103/1687-4293.175556
[4] Menzel MY. The Cytotaxonomy and Genetics of Physalis, Proceedings of the American Philosophical Society, 95 (2): 132-183, 1951.
https://www.jstor.org/stable/3143331
[5] Popenoe H, King S, León J, Kalinowski L. Goldenberry (Cape Gooseberry), in Lost Crops of the Incas, H. Popenoe, S. King, J. León, and L. Kalinowski, Eds. Washington, USA, National Academy Press, 1989, 415.
[6] Valdenegro M, Almonacid S, Henríquez C, Lutz M, Fuentes L, Simpson R. Effects of drying processes on organoleptic characteristics and the health quality of food ingredients obtained from goldenberry fruits (Physalis peruviana), Open Access Scientific Reports, 2: 642, 2013.
https://www.omicsonline.org/scientific-reports/srep642.php
[7] González C, Barrero LS. Estudio de la marchitez vascular de la uchuva para el mejoramiento genético del cultivo. Bogotá, Colombia, Editorial Kimpress, 2011.
[8] Simbaqueba J, Catanzariti A, González C, Jones D. Evidence for horizontal gene transfer and separation of effector recognition from effector function revealed by analysis of effector genes shared between cape-gooseberry- and tomato-infecting formae speciales of Fusarium oxysporum, Molecular Plant Pathology, 22: 2017.
doi: 10.1111/mpp.12700
[9] Osorio-Guarín JA, Enciso-Rodríguez FE, González C, Fernández-Pozo N, Mueller LA, Barrero LS. Association analysis for disease resistance to Fusarium oxysporum in cape gooseberry (Physalis peruviana L), BMC Genomics, 17 (1): 248, 2016.
doi: 10.1186/s12864-016-2568-7
[10] García-Arias FL, Osorio-Guarín JA, Núñez Zarantes VM. Association Study Reveals Novel Genes Related to Yield and Quality of Fruit in Cape Gooseberry (Physalis peruviana L.), Frontiers in Plant Science, 9:362, 2018.
doi: 10.3389/fpls.2018.00362
[11] Lee WS, Rudd JJ, Kanyuka K. Virus induced gene silencing (VIGS) for functional analysis of wheat genes involved in Zymoseptoria tritici susceptibility and resistance, Fungal Genetics and Biology, 79: 84-88, 2015.
doi: 10.1016/j.fgb.2015.04.006
[12] Burch-Smith TM, Anderson JC, Martin GB, Dinesh-Kumar SP. Applications and advantages of virus-induced gene silencing for gene function studies in plants, The Plant Journal, 39 (5): 734-746, 2004.
doi: 10.1111/j.1365-313X.2004.02158.x
[13] Zhang N, Huo W, Zhang L, Chen F, Cui D. Identification of Winter-Responsive Proteins in Bread Wheat Using Proteomics Analysis and Virus-Induced Gene Silencing (VIGS), Molecular & Cellular Proteomics, 15 (9): 2954-2969, 2016.
doi: 10.1074/mcp.M115.057232
[14] Fantini E, Giuliano G. Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry, in Plant Signal Transduction: Methods and Protocols, J. R. Botella and M. A. Botella, Eds. New York, NY, Springer New York, 65-78, 2016.
doi: 10.1007/978-1-4939-3115-6_7
[15] Groszyk J, Kowalczyk M, Yanushevska Y, Stochmal A, Rakoczy-Trojanowska M, Orczyk W. Identification and VIGS-based characterization of Bx1 ortholog in rye (Secale cereale L.), PLOS ONE, 12 (2): e0171506, 2017.
doi: 10.1371/journal.pone.0171506
[16] Kawalek A, Dmochowska-Boguta M, Nadolska-Orczyk A, Orczyk W. A new BSMV-based vector with modified β molecule allows simultaneous and stable silencing of two genes, Cellular & molecular biology letters, 17 (1): 107-23, 2012.
doi: 10.2478/s11658-011-0041-9
[17] Bennypaul HS, Mutti JS, Rustgi S, Kumar N, Okubara PA, Gill KS. Virus-induced gene silencing (VIGS) of genes expressed in root, leaf, and meiotic tissues of wheat, Functional & Integrative Genomics, 12 (1): 143-156, 2012.
doi: 10.1007/s10142-011-0245-0
[18] Voinnet O. RNA silencing as a plant immune system against viruses, Trends in Genetics, 17 (8): 449-459, 2001.
doi: 10.1016/S0168-9525(01)02367-8
[19] Donaire L, Wang Y, Gonzalez-Ibeas D, Mayer KF, Aranda MA, Llave C. Deep-sequencing of plant viral small RNAs reveals
effective and widespread targeting of viral genomes, Virology, 392
(2): 203-214, 2009.
doi: 10.1016/j.virol.2009.07.005
[20] Ding S-W, Voinnet O. Antiviral Immunity Directed by Small RNAs, Cell, 130 (3): 413-426, 2007.
doi: 10.1016/j.cell.2007.07.039
[21] Baulcombe D. RNA silencing in plants, Nature, 431 (7006): 356-363, Sep. 2004.
doi: 10.1038/nature02874
[22] Lange M, Yellina AL, Orashakova S, Becker A. Virus-Induced Gene Silencing (VIGS) in Plants: An Overview of Target Species and the Virus-Derived Vector Systems, , in Virus-Induced Gene Silencing: Methods and Protocols, A. Becker, Ed. Totowa, NJ, Humana Press, 2013, 1-14.
doi: 10.1007/978-1-62703-278-0_1
[23] Senthil-Kumar M, Mysore KS. Tobacco rattle virus-based virusinduced gene silencing in Nicotiana benthamiana, Nature Protocols, 9 (7): 1549-1562, 2014.
doi: 10.1038/nprot.2014.092
[24] Cai X-Z, Xu Q-F, Wang C-C, Zheng Z. Development of a Virus-Induced Gene-Silencing System for Functional Analysis of the RPS2-Dependent Resistance Signalling Pathways in Arabidopsis, Plant Molecular Biology, 62 (1-2): 223-232, 2006.
doi: 10.1007/s11103-006-9016-z
[25] Ratcliff F, Martin-Hernandez AM, Baulcombe DC. Technical Advance: Tobacco rattle virus as a vector for analysis of gene function by silencing, The Plant Journal, 25 (2): 237-245, 2001.
doi: 10.1046/j.0960-7412.2000.00942.x
[26] Kumagai MH, Donson J, della-Cioppa G, Harvey D, Hanley K, Grill LK. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA, Proceedings of the National Academy of Sciences of the United States of America, 92 (5): 1679-1683, 1995.
[27] Fernandez-Pozo N, Rosli HG, Martin GB, Mueller LA. The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing (VIGS) Constructs for Functional Genomics, Molecular Plant, 8 (3): 486-488, 2015.
doi: 10.1016/j.molp.2014.11.024
[28] Dong Y, Burch-Smith TM, Liu Y, Mamillapalli P, Dinesh-Kumar SP. A Ligation-Independent Cloning Tobacco Rattle Virus Vector for High-Throughput Virus-Induced Gene Silencing Identifies Roles for NbMADS4-1 and -2 in Floral Development, Plant Physiology, 145 (4): 1161-1170, 2007.
doi: 10.1104/pp.107.107391
[29] Liu Y, Schiff M, Marathe R, Dinesh-Kumar SP. Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus, The Plant Journal, 30 (4): 415-429, 2002.
doi: 10.1046/j.1365-313X.2002.01297.x
[30] Liu Y, Schiff M, Dinesh-Kumar SP. Virus-induced gene silencing in tomato, The Plant Journal, 31 (6): 777-786, 2002.
doi: 10.1046/j.1365-313X.2002.01394.x
[31] Liu E, Page JE. Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus, Plant Methods, 4(1): 5, 2008.
doi: 10.1186/1746-4811-4-5
[32] Velásquez AC, Chakravarthy S, Martin GB. Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato, Journal of Visualized Experiments JoVE, (28): 1292, 2009.
doi: 10.3791/1292
[33] Romero I, Tikunov Y, Bovy A. Virus-induced gene silencing in detached tomatoes and biochemical effects of phytoene desaturase gene silencing, Journal of Plant Physiology, 168 (10): 1129-1135, 2011.
doi: 10.1016/j.jplph.2010.12.020
[34] Chung E, Seong E, Kim Y-C, Chung EJ, Oh S-K, Lee S, Park JM, Joung YH, Choi D. A Method of High Frequency Virusinduced Gene Silencing in Chili Pepper (Capsicum annuum L. cv. Bukang), Mol. Cells, 17 (2): 377-380, 2004.
[35] Brigneti G, Martín-Hernández AM, Jin H, Chen J, Baulcombe DC, Baker B, Jones JDG. Virus-induced gene silencing in Solanum species, The Plant Journal, 39 (2): 264-272, 2004.
doi: 10.1111/j.1365-313X.2004.02122.x
[36] Chen J-C, Jiang C-Z, Reid MS. Silencing a prohibitin alters plant development and senescence, The Plant Journal, 44 (1): 16-24, 2005.
doi: 10.1111/j.1365-313X.2005.02505.x
[37] Pang J, Zhu Y, Li Q, Liu J, Tian Y, Liu Y, Wu J. Development of Agrobacterium-Mediated Virus-Induced Gene Silencing and Performance Evaluation of Four Marker Genes in Gossypium barbadense, PLOS ONE, 8 (9): e73211, 2013.
doi: 10.1371/journal.pone.0073211
[38] Meng L-H, Wang R-H, Zhu B-Z, Zhu H-L, Luo Y-B, Fu D-Q. Efficient Virus-Induced Gene Silencing in Solanum rostratum, PLOS ONE, 11 (6): e0156228, 2016.
doi: 10.1371/journal.pone.0156228
[39] Zhang J, Yu D, Zhang Y, Liu K, Xu K, Zhang F, Wang J, Tan G, Nie X, Ji Q, Zhao L, Li C. Vacuum and Co cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize, Frontiers in Plant Science, 8: 1-12, 2017.
doi: 10.3389/fpls.2017.00393
[40] Kirigia D, Runo S, Alakonya A. A virus-induced gene silencing (VIGS) system for functional genomics in the parasitic plant Striga hermonthica, Plant Methods, 10 (1): 16, 2014.
doi: 10.1186/1746-4811-10-16
[41] Yan H, Fu D, Zhu B, Liu H, Shen X, Luo Y. Sprout vacuuminfiltration: a simple and efficient agroinoculation method for virus-induced gene silencing in diverse solanaceous species, Plant Cell Reports, 31 (9): 1713-1722, 2012.
doi: 10.1007/s00299-012-1285-1
[42] Xu H, Xu L, Yang P, Cao Y, Tang Y, He G, Yuan S, Ming J. Tobacco rattle virus-induced Phytoene desaturase (PDS) and Mg-chelatase H subunit (ChlH) gene silencing in Solanum pseudocapsicum L., PeerJ, 6 e4424, 2018.
doi: 10.7717/peerj.4424
[43] Wang T, Wen L, Zhu H. Effectively organ-specific virus induced gene silencing in tomato plant, Journal of Nature and Science (JNSCI), 1 (1): 34, 2015.
How to Cite
Osorio-Guarín, J. A., García-Arias, F. L., & Yockteng, R. (2019). Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae). Universitas Scientiarum, 24(1), 111–133. https://doi.org/10.11144/Javeriana.SC24-1.vigs
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Section
Molecular Biology