Published Jul 12, 2021


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Maximiliano Rugolo

Juan Manuel Roggero-Luque

Mario Rajchenberg

Carolina Barroetaveña



This work evaluated mycelial growth rate (Kr) and fruiting of two Pleurotus ostreatus commercial strains (A01, 129) on formulations composed of lignocellulosic residues from farming and agroindustry of Northern Patagonian Andes, and of woody materials from invasive plants. Rosehip fluffs (RF), rosehip woodchips (RWC), southern beech wood shavings (SBWS), wheat straw (WS), and willow woodchips (WWC) were used as base substrates, and brewing bagasse (BB) as an alternative supplement to wheat bran (WB). Kr was higher in WS-WB and WS-BB for both strains. Experiments in fruiting chambers showed biological efficiencies (BEs) above 40% in WS-BB (both strains) and WS-WB (strain 129). Formulations using RWC or WWC gave BEs under 40%, while those composed of SBWS or RF showed lower Kr and contamination by moulds. Medium-scale fruiting experiments using strain A01 showed the highest BEs in WS-BB and RWC-WB. These results suggest that WS is the best substrate for Pleurotus ostreatus culture, although scarce in Northern Patagonian Andes. Nevertheless, WWC and RWC are suggested as alternative substrates, while BB is cheap and abundant, suitable as an alternative supplement to WB.


Pleurotus ostreatus, Patagonia, edible mushrooms, brewing bagasse

[1] Zervakis GI, Koutrotsios G, Katsaris P. Composted versus raw olive mill waste as substrates for the production of medicinal mushrooms: an assessment of selected cultivation and quality parameters, BioMed Research International: 1–13, 2013.
doi: 10.1155/2013/546830

[2] Selvi S, Devi PU, Suja S, Murugan S, Chinnaswamy P. Comparison of non-enzymic antioxidant status of fresh and dried form of Pleurotus florida and Calocybe indica, Pakistan Journal of Nutrition, 6: 468–71, 2007.
doi: 10.3923/pjn.2007.468.471

[3] Jaramillo S, Albertó E. Heat treatment of wheat straw by immersion in hot water decreases mushroom yield in Pleurotus ostreatus, Revista Iberoamericana de Micología, 30: 125–129, 2013.
doi: 10.1016/j.riam.2012.11.004

[4] Albertó E, Curvetto N, Deschamps J, González Matute R, Lechner B. Hongos Silvestres y de Cultivo en Argentina: Historia, regiones y sistemas de producción, hongos silvestres de valor económico, consumo, mercado interno y externo, legislación, oferta tecnológica e investigación y desarrollo. In: Martínez-Carrera D, Curvetto N, Sobal M, Morales P, Mora
VM, editors. Hacia un desarrollo sostenible del sistema de producción-consumo de los hongos comestibles y medicinales en Latinoamérica: avances y perspectivas en el siglo XXI, chapter 19. Red Latinoamericana de Hongos Comestibles y Medicinales, Puebla: 333–358, 2010.

[5] Salmones D, Mata G, Waliszewski KN. Comparative culturing of Pleurotus spp. on coffee pulp and wheat straw: biomass production and substrate biodegradation, Bioresource Technology, 96: 537–544, 2005.
doi: 10.1016/j.biortech.2004.06.019

[6] Sardar H, Ali MA, Anjum MA, Nawaz F, Hussain S, Naz S, Karimi SM. Agro-industrial residues influence mineral elements accumulation and nutritional composition of king oyster mushroom (Pleurotus eryngii). Scientia Horticulturae, 225: 327–334, 2017.
doi: 10.1016/j.scienta.2017.07.010

[7] Rugolo M, Levin L, Lechner B. Flammulina velutipes: an option for “alperujo” use, Revista Iberoamericana de Micología, 33: 242–247, 2016.
doi: 10.1016/j.riam.2015.12.001

[8] Mintesnot B, Ayalew A, Kebede A. Evaluation of biomass of some invasive weed species as substrate for oyster mushroom (Pleurotus spp.) cultivation, Pakistan journal of biological sciences: Pakistan Journal of Biological Sciences, 17: 213–219, 2014.
doi: 10.3923/pjbs.2014.213.219

[9] González AD. Energy and carbon embodied in straw and clay wall blocks produced locally in the Andean Patagonia, Energy and Buildings, 70: 15–22, 2014.
doi: 10.1016/j.enbuild.2013.11.003

[10] Danklmaier C, Heinrichs W, Riveros H. Activación territorial con enfoque de sistemas agroalimentarios localizados (AT-SIAL): la Comarca Andina del Paralelo 42. Argentina, Instituto Interamericano de Cooperación para la Agricultura (IICA), México: 80 pp, 2013.

[11] Mussatto SI, Dragone G, Roberto IC. Brewers’ spent grain: generation, characteristics and potential applications, Journal of Cereal Science, 43: 1–14, 2006.
doi: 10.1016/j.jcs.2005.06.001

[12] Wang D, Akiyoshi S, Motoyuki S. Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent beer grain, Bioresource Technology, 78: 293–300, 2001.
doi: 10.1016/s0960-8524(01)00002-5

[13] Gregori A, vagelj M, Pahor B, Berovi M, Pohleven F. The use of spent brewery grains for Pleurotus ostreatus cultivation and enzyme production, New Biotechnology, 25: 157–161, 2008.
doi: 10.1016/j.nbt.2008.08.003

[14] Carabelli F, Bava J, Momberg F, Cordone V. Estimación de volumen maderable en bosques de Lenga (Nothofagus pumilio) en la región patagónica de Argentina, Bosque, 25: 3–9, 2004.
doi: 10.4067/s0717-92002004000300001.

[15] Villena P. Capacidad instalada foresto-industrial Tierra del Fuego. Informe sobre las características sectoriales, cadena de producción y costos genéricos mediante relevamiento de las unidades productivas, Programa de Competitividad y gestión sustentable de las cadenas productivas TDF. Tierra del Fuego/BID-Fomin: 35 pp, 2013.

[16] Loguercio G, Jovanoski A, Molina J, Pantaenius P. Residuos de biomasa de forestaciones y aserraderos de la región andina de las provincias de Neuquén y Chubut: evaluación preliminar de oferta. Informe final CIEFAP y Secretaria de ambiente y desarrollo sustentable de la Nación, Argentina: 70 pp, 2008.

[17] Barros Biscari RM. Los saberes colectivos locales como factores del anclaje territorial. El SIAL de la Rosa Mosqueta rubiginosa de la Patagonia Argentina. In: 116th Seminar "Spatial Dynamics in Agri-food Systems: Implications for Sustainability and Consumer Welfare", European Association of Agricultural Economists. Parma, Italia: 1–9, 2010.
doi: 10.22004/ag.econ.95326

[18] Thomas LK, Mosner E, Leyer I. River dynamics and invasion: distribution patterns of native and invasive woody vegetation at the Río Negro, Argentina, Riparian Ecology and Conservation, 2: 45–57, 2015.
doi: 10.1515/remc-2015-0001

[19] Datri L, Faggi A, Gallo L. Crack willow changing riverine landscapes in Patagonia, Ecohydrology, 10: e1837, 2017.
doi: 10.1002/eco.1837.

[20] U.S. EPA. Microwave assisted acid digestion of siliceous and organically based matrices. Method 3052, Revision 0. In: SW-846. Test methods for evaluating solid waste, physical/chemical methods. Office of Solid Waste, Washington, DC, 1996.

[21] Martin TD, Brockhoff CA, Creed JT. Determination of metals and trace elements in water and wastes by inductively coupled plasma-atomic emission spectrometry. In: Method 200.7, Revision 4.4. Environmental Monitoring Systems Laboratory, Office of Research and Development, US Environmental Protection Agency, Cincinnati, Ohio, USA: 31–72, 1994.

[22] Muez MA, Pardo J. La preparación del substrato. In: Sánchez JE & Royse DJ, editors. La biología y el cultivo de Pleurotus spp. Limusa, México: 157–186, 2002.

[23] Rugolo M, Lechner B, Mansilla R, Mata G, Rajchenberg M. Evaluation of Pleurotus ostreatus basidiomes production on Pinus sawdust and other agricultural and forestry wastes from Patagonia, Argentina. Maderas, Ciencia y tecnología, 22: 517–526, 2020.
doi: 10.4067/S0718-221X2020005000410

[24] Philippoussis AN, Diamantopoulou PA, Zervakis GI. Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinula edodes, World Journal of Microbiology and Biotechnology, 19: 551–557, 2003.
doi: 10.1023/a:1025100731410

[25] Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW. InfoStat versión 2017. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina, 2017.

[26] Picornell MR, Pardo-Giménez A, Navarro MJ, Gea FJ. Actualizaciones sobre la preparación del sustrato para cultivar setas Pleurotus spp. In: Sánchez JE, Royse DJ, editors. La biología, el cultivo y las propiedades nutricionales y medicinales de las setas Pleurotus spp. San Cristóbal de Las Casas, Chiapas, México, El Colegio de la Frontera Sur: 83–104, 2017.

[27] Philippoussis A, Diamantopoulou P, Israilides C. Productivity of agricultural residues used for the cultivation of the medicinal fungus Lentinula edodes. International Biodeterioration & Biodegradation, 59: 216–219, 2007.
doi: 10.1016/j.ibiod.2006.10.007

[28] Gaitán-Hernández R, Salmones D. Uso de residuos lignocelulósicos para optimizar la producción de inóculo y la formación de carpóforos del hongo comestible Lentinula boryana, Revista Mexicana de Ciencias Agrícolas, 6: 1639–1652, 2015.
doi: 10.29312/remexca.v6i7.556.

[29] Curvetto NR, Figlas D, Devalis R, Delmastro S. Growth and productivity of different Pleurotus ostreatus strains on sunflower seed hulls supplemented with N–NH4+ and/or Mn (II), Bioresource Technology, 84: 171–176, 2002.
doi: 10.1016/s0960-8524(02)00013-5

[30] Picornell-Buendía MR, Pardo Giménez A, de Juan Valero JA. Reutilización del sustrato degradado de Pleurotus ostreatus. Parámetros cuantitativos. Información Técnica Económica Agraria, 112: 357–374, 2016.
doi: 10.12706/itea.2016.022

[31] Lechner BE, Albertó E. Search for new naturally occurring strains of Pleurotus to improve yields. Pleurotus albidus as a novel proposed species for mushroom production, Revista Iberoamericana de Micologia, 28: 148–154, 2011.
doi: 10.1016/j.riam.2010.12.001

[32] Garuba T, Abdukkareem KA, Ibrahim IA, Oyebamiji OI, Shoyooye OA, Ajibade TD. Influence of substrates on the nutritional quality of Pleurotus pulmonarius and Pleurotus ostreatus, Ceylon Journal of Science, 46: 67–74, 2017.
doi: 10.4038/cjs.v46i1.7419

[33] Velu C, Veeramani E, Suntharam S, Kalimuthu K. Insilico screening and comparative study on the effectiveness of textile dye decolourization by crude laccase immobilised alginate encapsulated beads from Pleurotus ostreatus, Journal of Bioprocessing & Biotechniques, 01: 109, 2011.
doi: 10.4172/2155-9821.1000109

[34] Pire DG, Wright JE, Alberto E. Cultivation of shiitake using sawdust from widely available local woods in Argentina, Micología Aplicada International, 13: 87–91, 2001.

[35] Eslyn WE, Lombard FF. Fungi associated with decayedwood in stored willowand cottonwood logs, Mycologia, 76: 548–550, 1984.
doi: 10.2307/3793339.

[36] Venturella G, Gargano ML, Compagno R. The genus Pleurotus in Italy, Flora Mediterranea 25 (Special Issue): 143–156, 2015.
doi: 10.7320/flmedit25si.143.

[37] Lechner BE, Wright JE, Albertó E. The genus Pleurotus in Argentina, Mycologia, 96: 845–858, 2004.
doi: 10.2307/3762117

[38] Zervakis G, Philippoussis A, Ioannidou S, Diamantopoulou P. Mycelium growth kinetics and optimal temperature conditions for the cultivation of edible mushroom species on lignocellulosic substrates, Folia Microbiologica, 46: 231–234, 2001.
doi: 10.1007/bf02818539

[39] Girmay Z, Gorems W, Birhanu G, Zewdie S. Growth and yield performance of Pleurotus ostreatus (Jacq. Fr.) Kumm (oyster mushroom) on different substrates, AMB Express, 6: 87, 2016.
doi: 10.1186/s13568-016-0265-1

[40] Li H, Zhang Z, Li M, Li X, Sun Z. Yield, size, nutritional value, and antioxidant activity of oyster mushrooms grown on perilla stalks, Saudi Journal of Biological Sciences, 24: 347–354, 2017.
doi: 10.1016/j.sjbs.2015.10.001

[41] Ahmed M, Abdullah N, Ahmed KU, Bhuyan MHM. Yield and nutritional composition of oyster mushroom strains newly introduced in Bangladesh, Pesquisa Agropecuária Brasileira, 48: 197–202, 2013.
doi: 10.1590/s0100-204x2013000200010

[42] Hoa HT, Wang CL, Wang CH. The effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus), Mycobiology, 43: 423–434, 2015.
doi: 10.5941/myco.2015.43.4.423

[43] Fanadzo M, Zireva DT, Dube E, Mashingaidze AB. Evaluation of various substrates and supplements for biological efficiency of Pleurotus sajor-caju and Pleurotus ostreatus, African Journal of Biotechnology, 9: 2756–2761, 2010.
doi: 10.5897/AJB09.1259

[44] Alam N, Amin R, Khair A, Lee TS. Influence of different supplements on the commercial cultivation of milky white mushroom, Mycobiology, 38: 184–188, 2010.
doi: 10.4489/myco.2010.38.3.184
How to Cite
Rugolo, M., Roggero-Luque, J. M., Rajchenberg, M., & Barroetaveña, C. (2021). Assessment of lignocellulosic residues from Northern Patagonian Andes (Argentina) for cultivation of Pleurotus ostreatus. Universitas Scientiarum, 26(2), 159–177.
Applied Microbiology