Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens
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Sep 27, 2023
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Abstract
Environmental factors affect nearly all land areas on the planet. Global warming is one of the most destructive of these factors because it has adverse effects on crop production systems. Plants are sessile organisms that have evolved complex mechanisms to cope with stress factors. Heat shock proteins (HSPs) are one of those mechanisms. In this study, we analyzed a partial gene sequence that encodes for HSP70 protein in Vasconcellea pubescens. We also measured the relative expression of the gene in plantlets of Vasconcellea pubescens and performed biochemical assays under heat stress. The plantlets were exposed to three temperatures 25° C (control), 45 °C and 55 °C (stress temperatures) for 4 hours. The bioinformatic analysis led to the first description of a partial sequence of the HSP70 gene and its evolutionary history in V. pubescens. We found significant differences for relative expression of theHSP70 gene, percentage of electrolyte leakage, and proline content between plants subjected to heat stress and those in the control group. Our results showed that V. pubescens displays thermotolerance even under extreme temperatures. V. pubescens is a poorly studied species that may contain genes of biotechnological interest (such as HSP70) that could be used for plant genetic modification.
Keywords
HSP70, Vasconcella pubescens, thermal stress, global warming, thermotolerant
References
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doi: 10.1016/j.wace.2015.08.001
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doi: 10.1111/pce.12417
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doi: 10.1016/j.ydbio.2016.07.023
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doi: 10.1371/journal.pone.0000076
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doi: 10.1111/pbi.12659
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doi: 10.1016/B978-0-7216-0186-1.50010-7
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doi: 10.1038/s41580-019-0133-3
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doi: 10.1111/pce.12396
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doi: 10.1016/j.tibs.2017.10.002
[14] Sarkar NK, Kundnani P, Grover A. Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa), Cell Stress and Chap, 18(4), 427–437, 2013.
doi: 10.1007/s12192-012-0395-6
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doi: 10.1007/s00018-004-4464-6
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doi: 10.1038/87588
[17] Peña D, Villena P, Aguirre AJ, Jiménez C. Diversidad genética de accesiones de la familia Caricaceae en el sur de Ecuador. Maskana, 8(1), 85–102, 2017.
doi: 10.18537/mskn.08.01.08
[18] Tineo D, Bustamante DE, Calderon MS, Mendoza JE, Huaman E, Oliva M. An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru. PLoS One. 2020 Dec 10;15(12):e0242469.
doi: 10.1371/journal.pone.0242469
[19] Scheldeman X, Kyndt T, Geo C, Ming R, Rod D, Van Droogenbroeck B, Moore PH. Vaconcellea, In: Kole C (Ed), Wild Crop Relatives; Genomic and Breeding Resources, 2011. Springer: Heidelber, Germany. p. 213–249.
doi: 10.1007/978-3-642-20447-0
[20] Alvarez E, Chan-León A, Girón-Ramírez A, Fuentes G, Estrella-Maldonado H, Santamaría JM. Genome-Wide Analysis of WRKY and NAC Transcription Factors in Carica papaya L. and Their Possible Role in the Loss of Drought Tolerance by Recent Cultivars through the Domestication of Their Wild Ancestors, Plants 2023, 12:2275, 2023.
doi: 10.3390/plants12152775
[21] Gaete-Eastman C, Figueroa CR, Balbontín C, Moya M, Atkinson RG, Herrera R, Moya-León MA. Expression of an ethylene-related expansin gene during softening of mountain papaya fruit (Vasconcellea pubescens), Postharv Biol and Tech, 53(1–2), 58–65, 2009.
doi: 10.1016/j.postharvbio.2009.03.007
[22] Arizala-Quinto ED, Viteri G, Idrovo-Espín, FM. Partial sequences of the gene that codifies for the transcription factor VPHSFB1 in Vasconcellea pubescens: First report. BAG. Journ of basic and app gen, 30(1): 7-9, 2019.
doi: 10.35407/V.30-1-2019
[23] Cevallos-Vilatuña T, Garzón KA, Idrovo-Espín FM. Internal control gene VpEf1α in Vasconcellea pubescens (chamburo). Rev Am Ciencia y Tecn, 8(1), 1-11, 2019.
[24] Arce M. Normal climática y distribución de la precipitación de la hacienda El Prado-IASA, Bol. Téc. 8, Ser. Zool. 4-5: 126–128, 2009.
[25] Goodstein DM, Shu S, Howson R, Neupane R, Hayes RD, Fazo J, Mitros T, Dirks W, Hellsten U, Putnam N, Rokhsar DS. Phytozome: a comparative platform for green plant genomics, Nucleic Acids Res. 40:D1178-86, 2012.
doi: 10.1093/nar/gkr944
[26] Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput, Nucleic Acids Res. 19,32(5):1792-7, 2004.
doi: 10.1093/nar/gkh340
[27] Tamura K, Stecher G, Kumar S. MEGA11: Molecular Evolutionary Genetics Analysis Version 11, Mol. Biol. Evol., 38(7):3022–3027, 2021.
doi: 10.1093/molbev/msab120
[28] Stanke M, Steinkamp R, Stephan Waack S, Morgenstern B. AUGUSTUS: a web server for gene finding in eukaryotes, Nucleic Acids Res, 32:W309-W312, 2004.
doi: 10.1093/nar/gkh379
[29] Crooks GE, Hon G, Chandonia JM, Brenner SE. WebLogo: A sequence logo generator, Gen Res, 14:1188-1190, 2004.
doi: 10.1101/gr.849004
[30] Camejo D, Rodríguez P, Morales M, Dell’Amico J, Torrecillas A, Alarcón J. High temperature effects on photosynthetic activity of two tomato cultivars with different heat susceptibility, Journ of Plant Phys, 162: 281-289, 2005.
doi: 10.1016/j.jplph.2004.07.014
[31] Aebi H. Catalase in vitro, Methods Enzymology, 105:121-126, 1984.
[32] Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil 39, 205–207 (1973).
doi: 10.1007/BF00018060
[33] Fox J. The R Commander: A Basic Statistics Graphical User Interface to R, Journ of Stat Soft, 14(9):1–42, 2005.
doi: 10.18637/jss.v014.i09
[34] Scheldeman X, Willemen L, Coppens D’Eeckenbrugge G, Romeijn-Peeters E, Restrepo M T, Romero Motoche J, Goetgebeur P. Distribution, diversity and environmental adaptation of highland papayas (Vasconcellea spp.) in tropical and subtropical America, Biodiv and Cons, 16(6), 1867–1884, 2007.
doi: 10.1007/s10531-006-9086-x
[35] Purwito A, Wattimena GA, Syukur M. Evaluation of potato clones for their adaptation to medium altitude conditions in the tropics, Euphytica, 213:237, 2017.
doi: 10.1007/s10681-017-2022-1
[36] Rykaczewska K. Impact of heat and drought stresses on size and quality of the potato yield, Plant, Soil and Env, 63(1):40-46, 2017.
doi: 10.17221/691/2016-PSE
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How to Cite
Idrovo, F., & Cevallos-Vilatuña, T. (2023). Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens. Universitas Scientiarum, 28(3), 279–298. https://doi.org/10.11144/Javeriana.SC283.psaa
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Molecular Biology
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