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Abstract
Only 3.5% of the global land area is not affected by some type of environmental factor. The increase in temperature due to global warming has negative implications on global crop production systems. However, plants are complex organisms, able to develop tolerance to different stress factors. Considering the negative impact on crop fields, the general objective of this work was to analyze a partial gene sequence and the relative expression of a gene coding for HSP70 in seedlings of Vasconcellea pubescens subjected to thermal stress. The plantlets were subjected to 25° C, as control temperature, and to 45 ° C and 55 ° C as stress temperatures for 4 hours. From the bioinformatics analysis, a partial sequence of HSP70, and its evolutionary history in V. pubescens was first described. The analysis of variance (ANOVA), showed significance for relative expression of HSP70 gene, electrolyte leak and proline content. Our results showed that V. pubescens behaves as thermotolerant against temperatures, considered as extreme. V. pubescens is an underutilized plant that could harbor genes of interest that commercial plants have lost due to domestication.
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