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Nitric oxide (NO) and reactive oxygen species (ROS) levels have been associated with resistance to apoptosis, regulation of the immune response, resistance to therapy and proliferation of leukemic cells. Plant-derived extracts have the ability to modulate different metabolic mechanisms and decrease the chemoresistance of tumor cells. In this study, we evaluated the effect of plant-derived extracts P2Et (Caesalpinia spinosa), Anamú-SC (Petiveria alliacea) and their combination with chemotherapeutic agents on NO and ROS levels in leukemic cell lines K562 and Reh. The leukemic cell lines K562 (myeloid lineage) and Reh (lymphoid lineage) were used. Cell lines were treated with plant-derived extracts P2Et and Anamú-SC for 12 hours. Additionally, K562 was treated with Idarubicin and Cytarabine and Reh with Doxorubicin, Vincristine and Methotrexate. NO and ROS were determined using the DAF-FM DA and lH2DCFDA probes. Mean fluorescence intensity for each variable was determined by flow cytometry. Anamú-SC and its combination with idarubicin induces an increase of NO levels in K562 cells. P2Et and Anamú-SC have an antioxidant effect on K562 and Reh cells with a significant decrease in ROS compared to basal levels. Anamú-SC and P2Et decrease the number of K562 and Reh cells after 12 hours of treatment without decreasing the viability of the cells. Plant-derived extracts P2Et and Anamú-SC can modulate NO and ROS levels in K562 and Reh cells. It is necessary to study in depth the effect of these extracts in the modulation of NO and ROS in primary human leukemic cells.
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Copyright (c) 2023 Juan José Arévalo-Ferrin, Jimmy Alejandro García-Ortiz, Cindy Mayerli Arévalo-Olaya, Sandra Milena Quijano-Gómez, Susana Fiorentino-Gómez, Viviana Marcela Rodríguez Pardo