Dendritic cell activity is an important step to elicit cellular immunity. These cells are the most potent antigen presenting cells and the main activating factor for naive T cells. There are several phenotypes of dendritic cells originated from different cell precursors. The aim of this study was to assess a model to obtain human myeloid dendritic cells from peripheral blood CD14+ monocytes. Cells were cultured in the presence of  granulocyte-monocyte colonies stimulating factor (GM-CSF) and IL-4 to obtain immature dendritic cells, wich were further cultured with PGE2 plus TNFa to induce their maturation. We obtained immature dendritic cells at  day 5 and mature dendritic cells at day 7, according to microscopic cellular
phenotype and positivity of membrane markers for CD14, CD86, HLA-DR and CD83,