Published Mar 10, 2004



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Adriana Cuéllar Ávila

Catherine Cifuentes Rojas

John Mario González Escobar

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Abstract
Dendritic cell activity is an important step to elicit cellular immunity. These cells are the most potent antigen presenting cells and the main activating factor for naive T cells. There are several phenotypes of dendritic cells originated from different cell precursors. The aim of this study was to assess a model to obtain human myeloid dendritic cells from peripheral blood CD14+ monocytes. Cells were cultured in the presence of  granulocyte-monocyte colonies stimulating factor (GM-CSF) and IL-4 to obtain immature dendritic cells, wich were further cultured with PGE2 plus TNFa to induce their maturation. We obtained immature dendritic cells at  day 5 and mature dendritic cells at day 7, according to microscopic cellular
phenotype and positivity of membrane markers for CD14, CD86, HLA-DR and CD83,
Keywords
References
How to Cite
Cuéllar Ávila, A., Cifuentes Rojas, C., & González Escobar, J. M. (2004). GENERACIÓN in vitro DE CÉLULAS DENDRÍTICAS HUMANAS DE ORIGEN MIELOIDE. Universitas Scientiarum, 9, 57–67. Retrieved from https://revistas.javeriana.edu.co/index.php/scientarium/article/view/5035
Section
Microbiología Clínica / Clinical Microbiology / Microbiologia Clinica

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