Published Oct 11, 2021



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Ana Isabel Giraldo Rivera http://orcid.org/0000-0002-4733-5460

Gloria Edith Guerrero-Álvarez https://orcid.org/0000-0002-0529-5835

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Abstract

Annona montana Macfad is a fruit species of the Annonaceae family. In this study, the phytochemical potential of A. montana seeds was investigated. Ethanol and hexane extracts from seeds were evaluated for cytotoxicity and insecticidal activity, phenolic content, and antioxidant capacity. The latter being related to free radical scavenging activity assay (DPPH) and ferric reducing power (FRAP). Exposing Artemia salina to both seed extract types reveled their high toxicity with a median lethal concentration (LC50) of < 10 μg mL-1. Further A. montana seed insecticidal activity was evaluated against Thrips tabaci L., revealing that the most promising treatments were observed for a concentration of 100 mg L-1 in both extracts. The ethanol extract resulted in a mortality of 67.5 % and the hexane extract in a 53.3 % mortality. The ethanolic extract of A. montana seeds showed the highest total phenolic content: 297.38 mg GAE/100 g of dried extract and 192.66 mg TE/100 g, and 385.46 mg TE/100 g for DPPH and FRAP, respectively. The chemical characterization of both extracts by high performance liquid chromatography (HPLC) revealed the presence of acetogenins. The results obtained idicate that the A. montana extracts are a promising source of compounds with insecticidal activity.

Keywords

Annonaceae, antioxidant, cytotoxicity, DPPH, FRAP, insecticidal activity

References
[1] Gonzales-Esquinca AR, De la cruz-Chacón I, Castro-Moreno M, Orozco-Castillo JA, Riley- Saldaña CA. Alkaloids and Acetogenins in Annonaceae Development: Biological Considerations, Revista Brasileira de Fruticultura, 36(esp1): 1–16, 2014.
doi: 10.1590/S0100-29452014000500001

[2] Lim. TK. Edible Medicinal and Non-Medicinal Plants: Fruits, Springer, 221–226, 2012.

[3] Barbalho S, Goulart R, Farinazzi-Machado FM, De Souza M, Do Santos Bueno PC, Guiguer E, Araújo A, Groppo M. Annona sp: Plants with Multiple Applications as Alternative Medicine - A Review, Current Bioactive Compounds, 8(3): 277–286, 2012.
doi: 10.2174/157340712802762500

[4] Colom OA, Barrachina I, Mingol IA, Gonzalez MC, Sanz PM, Neske A, Bardon A. Toxic effects of annonaceous acetogenins on Oncopeltus fasciatus, Journal of Pest Science, 81(2): 85–9, 2008.
doi: 10.1007/s10340-007-0189-2

[5] Chávez PI, Sánchez LA, González FA, Rodríguez JL, Axelrod F. Cytotoxicity correlations of Puerto Rican plants using a simplified brine shrimp lethality screening procedure, International Journal of Pharmacognosy, 35(4): 222–226, 1997.
doi: 0925-1618/97/3504-0222$12.00

[6] Bailon-Moscoso N, Romero JC, Ramirez MI, Ojeda K, Granda G, Ratoviski EA, Ostrosky- Wegman P. Cytotoxic and genotoxic effects of extracts from Annona montana M. fruit, Food and Agricultural Immunology, 6: 17, 2016.
doi: 10.1080/09540105.2016.1148121

[7] Ribeiro LP, Akhtar Y, Vendramim JD, Isman MB. Comparative bioactivity of selected seed extracts from Brazilian Annona species and an acetogenin-based commercial bioinsecticide against Trichoplusia ni and Myzus persicae, Crop Protection, 62: 100–6, 2014.
doi: 10.1016/j.cropro.2014.04.013

[8] Ribeiro LP, Vendramim JD, Andrade MS, Bicalho KU, Silva MFGF, Vieira PC, Fernandes JB. Tropical Plant Extracts as Source of Graint-Protectant Compounds Againt Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), Neotropical Entomology, 43: 470–482, 2014.
doi: 10.1007/s13744-014-0233-x

[9] Souza CM, Baldin ELL, Ribeiro LP, Silva IF, Morando R, Bicalho KU, Vendramim JD, Fernandes JB. Lethal and growth inhibitory activities of Neotropical Annonaceae-derived extracts, commercial formulation, and an isolated acetogenin against Helicoverpa armigera, Jorunal of Pest Science, 90(2): 701–9, 2017.
doi: 10.1007/s10340-016-0817-9

[10] Da Silva Bandeira HF, Silva AC, Trassato LB, Strucker A, De Jesus Vieira A. Bioactivity of Annona montana Macfad extracts on the black cowpea aphid (Aphis craccivora Koch), Revista Brasileirade Ciências Agrárias, 12(11): 41–6, 2017.
doi: 10.5039/agraria.v12i1a5419

[11] Di Toto L, Colom O, Popich S, Neske A, Bardón A. Antifeedant, and toxic effects of acetogenins from Annona montana on Spodoptera frugiperda, Journal Pest Science, 83: 307–310. 2010.
doi: 10.1007/s10340-010-0299-331

[12] Innocent E, Kisanga AC, Gamba N, Kisinza WN, Hassanali A. Effect of Annona formulations on non-target invertebrates and on physicochemical water parameters at semi-field condition, Journal of Entomology and Zoology Studies, 2(3): 214–219, 2014.

[13] Castillo LE, Jiménez JJ, Delgado MA. Secondary metabolites of the Annonaceae, Solanaceae
and Meliaceae families used as biological control of insects. Tropical and Subtropical Agroecosystems, 12(3): 445–462, 2010.

[14] Krinski D, Massaroli A, Machado M. Potential insecticide de plantas da familia Annonaceae. Revista Brasileira de Fruticultura, 36(spe1): 225–42, 2014.
doi: 10.1590/S0100-29452014000500027

[15] Azazy AM, Abdelall MFM, El-Sappagh IA, Khalil AEH. Biological control of the onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae), in open fields using Egyptian entomopathogenic nematode isolates, Egyptian Journal of Biological Pest Control, 28(1): 17, 2018.
doi: 10.1186/s41938-017-0025-9

[16] Lebedev G, Abo-Moch F, Gafni G, Ben-Yakir D, Ghanim M. High-level of resistance to spinosad, emamectin benzoate and carbosulfan in populations of Thrips tabaci collected in Israel. Pest Management Science, 69(2): 274–277, 2013.
doi: 10.1002/ps.3385.

[17] Zamar MI, Arce de Hamity MG, Andrade A, Amendola de Olsen A, Hamity V. Efecto de productos no convencionales para el control de Thrips Tabaci (Thysanoptera: Thripidae) en el cultivo de Ajo (Allium sativum) en la quebrada de Humahuaca (Jujuy-Argentina). Idesia
(Arica), 25(3): 41–46, 2007.
doi: 10.4067/S0718-34292007000300005

[18] Castro L, Alzate M, Guerrero G. Estudio preliminar de la bioactividad de extractos de semillas de Annona cherimolia de la familia annonaceae. Science and Techology XVI, 1(44): 326–330, 2010.
doi: 10.22517/23447214.1859

[19] Lagarto A, Silva R, Guerra I, Iglesias L. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts, Phytomedicine, 8(5): 395–400, 2001.
doi: 10.1078/0944-7113-00044

[20] McLaughlin JL, Rogers LL, Anderson JE. The Use of Biological Assays to Evaluate Botanicals, Drug Information Journal, 32(2): 513–524, 1998.
doi: 10.1177/009286159803200223

[21] Durán A, Onofre V, Aragón A, Rodríguez C, Tapia AM, Lopez-Olguín JF. Técnica de laboratorio para la cría de Thrips tabaci Lindeman (Thysanoptera: Thripidae), Avances en Agroecologia y Ambiente, I: 327–336, 2007.

[22] Herrera-Vásquez JÁ, Barba-Alvarado AA. Identificación de Thrips palmi (Thysanoptera: Thripidae) en cultivos de Cucurbitáceas en Panamá, Agronomia Mesoamericana, 24(1): 47–55, 2013.
doi: 10.15517/am.v24i1.9640

[23] Giraldo AI, Guerrero G, Arrubla JP, Baena LM, Cuervo DP, Gomez M. The effects of Annonaceae and Amaryllidaceae extracts in controlling the Thrips tabaci Lindeman (Thysanoptera: Thripidae), Revista Brasileira de Ciências Agrárias, 15(2): 1–9, 2020.
doi: 10.5039/agraria.v15i2a6933

[24] Magalhães LM, Santos F, Segundo MA, Reis S, Lima JLFC. Rapid microplate highthroughput methodology for assessment of Folin-Ciocalteu reducing capacity, Talanta, 83(2): 441–447. 2010.
doi: 10.1016/j.talanta.2010.09.042

[25] Ortiz J, Marín-Arroyo MR, Noriega-Domínguez MJ, Navarro M, Arozarena I. Color, phenolics, and antioxidant activity of blackberry (Rubus glaucus Benth.), blueberry (Vaccinium floribundum Kunth.), and apple wines from Ecuador, Journal of Food Science, 78(7): 985–993, 2013.
doi: 10.1111/1750-3841.12148

[26] Calderón-Oliver M, Escalona-Buendía HB, Medina-Campos ON, Pedraza-Chaverri J, Pedroza-Islas R, Ponce-Alquicira E. Optimization of the antioxidant and antimicrobial response of the combined effect of nisin and avocado byproducts, LWT - Food Science and
Technology, 65: 46–52, 2016.
doi: 10.1016/j.lwt.2015.07.048

[27] Giraldo A, Guerrero G. Rollinia mucosa (Jacq.) Baillon (Annonaceae) active metabolites as alternative biocontrol agents against the lace bug Corythucha gossypii (Fabricius): an insect pest, Universitas Scientarium, 23(1): 21–34, 2018.
doi: 10.11144/javeriana.sc23-1.rmjb

[28] Salas J, Morales G, Mendoza O, Álvarez C, Parra A. biología y hábitos de vida de Thrips tabaci Lindeman Thysanoptera: Thripidae en cebolla Allium cepa L, Agronomia Tropical, 43(3): 173–183, 1993.

[29] Vasco C, Ruales J, Kamal-Eldin A. Total phenolic compounds and antioxidant capacities of major fruits from Ecuador, Food Chemistry, 111(4): 816–823, 2008.
doi: 10.1016/j.foodchem.2008.04.054

[30] Colom OÁ, Neske A, Chahboune N, Zafra-Polo MC, Bardón A. Tucupentol, a novel monotetrahydrofuranic acetogenin from Annona montana, as a potent inhibitor of mitochondrial complex I, Chemistry & Biodiversity, 6(3): 335–340, 2009.
doi: 10.1002/cbdv.200800102

[31] Liaw C-C, Chang F-R, Chen S-L, Wu C-C, Lee K-H, Wu Y-C. Novel cytotoxic monotetrahydrofuranic Annonaceous acetogenins from Annona montana, Bioorganic & Medicinal Chemistry, 13(15): 4767–4776, 2005.
doi: 10.1016/j.bmc.2005.05.008

[32] Mullin C a, González-Coloma A, Gutiérrez C, Reina M, Eichenseer H, Hollister B, Chyb S. Antifeedant effects of some novel terpenoids on Chrysomelidae beetles: comparisons with alkaloids on an alkaloid-adapted and nonadapted species, Journal of Chemical Ecology, 23(7): 1851–1866. 1997.
doi: 10.1023/B:JOEC.0000006455.72602.3f

[33] Liaw CC, Chang FR, Wu YC, Wang HK, Nakanishi Y, Bastow KF, Lee KH. Montacin and cis-montacin, two new cytotoxic monotetrahydrofuran annonaceous acetogenins from Annona montana, Journal of Natural Products, 67(11): 1804–1808, 2004.
doi: 10.1021/np030216p

[34] Wang LQ, Zhao WM, Qin GW, Cheng KF, Yang RZ. Four novel annonaceous acetogenins from Annona montana, Natural Product Letters, 14(2): 83–90, 1999.
doi: 10.1080/10575639908041214

[35] Colom OA, Neske A, Popich S, Bardón A. Toxic effects of annonaceous acetogenins from Annona cherimolia (Magnoliales: Annonaceae) on Spodoptera frugiperda (Lepidoptera: Noctuidae), Journal of Pest Science, 80(1): 63–67, 2004.
doi: 10.1007/s10340-006-0149-2

[36] Ansante TF, do Prado Ribeiro L, Bicalho KU, Fernandes JB, das Graças Fernandes da Silva MF, Vieira PC, Vendramim JD. Secondary metabolites from Neotropical Annonaceae: Screening, bioguided fractionation, and toxicity to Spodoptera frugiperda (J.E. Smith)
(Lepidoptera: Noctuidae), Industrial Crops and Products, 74: 969–976, 2015.
doi: 10.1016/j.indcrop.2015.05.058

[37] Il-Kwon P, Hoi-Seon L, Sang-Gil L, Ji-Doo P, Young-Joon A. Antifeeding Activity of Isoquinoline Alkaloids Identified in Coptis japonica Roots Against Hyphantria cunea (Lepidoptera: Arctiidae) and Agelastica coerulea (Coleoptera: Galerucinae), Journal of Economic
Entomology, 93(2): 331–335, 2000.
doi: 10.1603/0022-0493-93.2.331

[38] Mann RS, Kaufman PE. Natural Product Pesticides: Their Development, Delivery and Use Against Insect Vectors, Mini-Reviews in Organic Chemistry, 9: 185–202, 2012.
doi: 10.2174/157019312800604733

[39] Palomino, Lady, García C, Gil J, Rojano B, Durango D. Determinación del contenido de fenoles y evaluación de la actividad antioxidante de propóleos recolectados en el departamento de Antioquia (Colombia), Viate, 16(3): 388–395, 2009.

[40] Fidyasari A, Wulandari S, Sari MI. Secondary metabolite, and antioxidant activity of soursop (Annona montana) fruit extract, International Journal of Technology Science, 1(2): 10–15, 2017.

[41] Barreiros ML, De Jesus RA, Barreiros ALBS, Sandes TS, Ramalho SA, Narain N. Evaluation of the antioxidant activity of eight tropical fruits by DPPH method, Acta Horticulturae, 1198: 185–192, 2018.
doi: 10.17660/ActaHortic.2018.1198.29

[42] Kuskoski M, Asuero A, Troncoso A, Mancini-FFilho J, Fett R. Aplicación de diversos métodos químicos para determinar actividad antioxidante en pulpa de frutos, Food Science and Technology, 25(4): 726–732, 2005.
doi: 10.1590/S0101-20612005000400016

[43] Barreca D, Laganà G, Ficarra S, Tellone E, Leuzzi U, Galtieri A, Bellocco E. Evaluation of the antioxidant and cytoprotective properties of the exotic fruit Annona cherimola Mill. (Annonaceae), Food Research International, 44(7): 2302–2310, 2011.
doi: 10.1016/j.foodres.2011.02.031

[44] Baskar R, RajeswaRi V, Sathish T. In vitro antioxidant studies in leaves of Annona species, Indian Journal of Experimental Biology, 45(5): 480–485, 2007.
doi: 10.1021/acs.jpcb.8b07859

[45] Khaliq A, Khan AA, Afzal M, Tahir HM, Raza AM, Khan AM. Field evaluation of selected botanicals and commercial synthetic insecticides against Thrips tabaci Lindeman (Thysanoptera: Thripidae) populations and predators in onion field plots, Crop Protection,
62: 10–15,2014.
doi: 10.1016/j.cropro.2014.03.019

[46] Satyanarayana P, Singh P. Relative field efficacy of botanicals, bio-pesticides, and synthetic insecticides against thrips (Thrips tabaci Lind) on onion, Journal of Experimental Zoology, 19(1): 191–194, 2016.
How to Cite
Giraldo Rivera, A. I., & Guerrero-Álvarez, G. E. (2021). Biological activities of Annona montana Macfad extracts. Universitas Scientiarum, 26(3), 302–316. https://doi.org/10.11144/Javeriana.SC26-3.baoa
Section
Productos Naturales / Natural Products / Produtos Naturais