Effects of natural compounds and commercial antibiotics on Pseudomonas aeruginosa quorum sensing ingles
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Dec 16, 2022
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Abstract
Pseudomonas aeruginosa is a Gram-negative bacterium designated by the WHO as a critical priority microorganism due to its virulence, controlled by a quorum sensing (QS) system. QS is regulated} through specific subsystems: LasI/LasR, RhlI/RhlR, and PQS/MvfR. Several natural compounds can inhibit these QS mechanisms. In this study, we determined the effect of curcumin, reserpine, and their mixtures with two commercial antibiotics (gentamicin and azithromycin) on P. aeruginosa QS mechanisms: mvfR gene expression and the production of pyocyanin and rhamnolipids. Antibiotic and natural compounds’ minimal inhibitory concentrations (MICs) were determined via microdilution assays. Gentamicin, azithromycin, curcumin, reserpine, and their mixtures exerted variable effects on mvfR gene expression, as assessed via semi-quantitative RT-PCR assays. Curcumin, reserpine, and gentamicin inhibited mvfR gene expression better than azithromycin, and the mixtures curcumin-gentamicin and reserpine-gentamicin outperformed gentamicin alone in inhibiting mvfR gene expression and decreasing pyocyanin and rhamnolipids production, revealing the synergistic effect of these mixture components. The mixtures of curcumin and gentamicin and reserpine and gentamicin may become alternatives to complement or enhance conventional methods currently used to treat P. aeruginosa infections.
Keywords
Curcumin, gene expression, Pseudomonas aeruginosa, Quorum sensing, reserpine, virulence factors
References
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Parai D, Banerjee M, Dey P, Chakraborty A, Islam E, Mukherjee SK. Effect of reserpine on Pseudomonas aeruginosa quorum sensing mediated virulence factors and biofilm formation. Biofouling, 34(3): 320–334, 2018.
doi: 10.1080/08927014.2018.1437910
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Savli H, Karadenizli A, Kolayli F, Gundes S, Ozbek U, Vahaboglu H. Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR. Journal of Medical Microbiology,
52(5): 403–408, 2003.
doi: 10.1099/jmm.0.05132-0
Thorn RMS, Nelson SM, Greenman J. Use of a Bioluminescent Pseudomonas aeruginosa Strain within an In Vitro Microbiological System, as a Model of Wound Infection, To Assess the Antimicrobial Efficacy of Wound Dressings by Monitoring Light Production. Antimicrobial Agents and Chemotherapy, 51(9): 3217–3224, 2007.
doi: 10.1128/AAC.00302-07
Tyagi P, Singh M, Kumari H, Kumari A, Mukhopadhyay K. Bactericidal activity of curcumin I is associated with damaging of bacterial membrane. PLoS ONE, 10(3): 1–151, 2015.
doi:10.1371/journal.pone.0121313
doi: 10.1016/J.PHYMED.2015.01.002
Abreu AC, McBain AJ, Simões M. Plants as sources of new antimicrobials and resistancemodifying agents. Natural Product Reports, 29(9): 1007–1021, 2012.
doi: 10.1039/c2np20035j
Ameer N, Zakariya B, Multan U, Hanif M, Azeem M, Mahmood Bahauddin K, University Z, Fazal M, Sajid R, Bahauddin C, Nasreen M, Bahauddin R, Mubashir M, Bahauddin A, Pandey MK. Synergistic antibacterial effect of highly permeable curcumin and ciprooxacin containing selfemulsifying drug delivery system against urinary tract bacterial infections. Research Square: 1–17, 2022.
doi: 10.21203/rs.3.rs-1551439/v1
Ansel HC, Norred WP, Roth IL. Quantification of pyocyanins was performed according to described in previous study. Journal of Pharmaceutical Sciences, 58(7): 836–839, 1969.
doi: 10.1002/JPS.2600580708
Azam MW, Khan AU. Updates on the pathogenicity status of Pseudomonas aeruginosa. Drug Discovery Today, 24(1): 350–359, 2019.
doi: 10.1016/J.DRUDIS.2018.07.003
Bahari S, Zeighami H, Mirshahabi H, Roudashti S, Haghi F. Inhibition of Pseudomonas aeruginosa quorum sensing by subinhibitory concentrations of curcumin with gentamicin and azithromycin. Journal of Global Antimicrobial Resistance, 10: 21–28, 2017.
doi: 10.1016/J.JGAR.2017.03.006
Banerjee M, Moulick S, Bhattacharya KK, Parai D, Chattopadhyay S, Mukherjee SK. Attenuation of Pseudomonas aeruginosa quorum sensing, virulence and biofilm formation by extracts of Andrographis paniculata. Microbial Pathogenesis, 113(August): 85–93, 2017.
doi: 10.1016/j.micpath.2017.10.023
Bédard E, Prévost M, Déziel E. Pseudomonas aeruginosa in premise plumbing of large buildings. MicrobiologyOpen, 5(6): 937–956, 2016.
doi: 10.1002/mbo3.391
Caesar LK, Cech NB, Kubanek J, Linington R, Luesch H. Synergy and antagonism in natural product extracts: when 1C1 does not equal 2. Natural Product Reports, 36(6): 869–888, 2019.
doi: 10.1039/C9NP00011A
Cockerill F, Wikler M, Hardy D. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement (M100-S23-2013). Clinical and Laboratory Standards Institute, 49(9): 745–754, 2013.
doi: 10.1586/14737167.2015.1087317
Davarzani F, Yousefpour Z, Saidi N, Owlia P. Different effects of sub-minimum inhibitory concentrations of gentamicin on the expression of genes involved in alginate production and biofilm formation of Pseudomonas aeruginosa. Iranian Journal of Microbiology, 13(6): 808–816, 2021.
doi: 10.18502/IJM.V13I6.8085
Déziel E, Gopalan S, Tampakaki AP, Lépine F, Padfield KE, Saucier M, Xiao G, Rahme LG. The contribution of MvfR to Pseudomonas aeruginosa pathogenesis and quorum sensing circuitry regulation: Multiple quorum sensing-regulated genes are modulated without affecting IasRI, rhIRI or the production of N-acyl-L-homoserine lactones. Molecular Microbiology, 55(4): 998–1014, 2005.
doi: 10.1111/J.1365-2958.2004.04448.X
Essar DW, Eberly L, Hadero A, Crawford IP. Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: Interchangeability of the two anthranilate synthase and evolutionary implications. Journal of Bacteriology, 172(2): 884–900, 1990.
doi: 10.1128/jb.172.2.884-900.1990
Feng L, Xiang Q, Ai Q, Wang Z, Zhang Y, Lu Q. Effects of Quorum Sensing Systems on Regulatory T Cells in Catheter-Related Pseudomonas aeruginosa Biofilm Infection Rat Models. Mediators of Inflammation, 2016.
doi: 10.1155/2016/4012912
Gibbons S. Anti-staphylococcal plant natural products. Pubs.Rsc.Org, 21(2): 263–277, 2004.
doi: 10.1039/b212695h
Inouye S, Tsuruoka T, Uchida K, Yamaguchi H. Effect of sealing and tween 80 on the antifungal susceptibility testing of essential oils. Microbiology and Immunology, 45(3): 201–208, 2001.
doi: 10.1111/j.1348-0421.2001.tb02608.x
Kali A, Bhuvaneshwar D, Charles PMV, Seetha KS. Antibacterial synergy of curcumin with antibiotics against biofilm producing clinical bacterial isolates. Journal of Basic and Clinical Pharmacy, 7(3): 93, 2016.
doi: 10.4103/0976-0105.183265
Kariminik A, Baseri-Salehi M, Kheirkhah B. Pseudomonas aeruginosa quorum sensing modulates immune responses: an updated review article. Inmunolohy letters, 190: 1–6, 2017.
doi: 10.1016/j.imlet.2017.07.002
Khameneh B, Iranshahy M, Soheili V, Fazly BBS. Review on plant antimicrobials: a mechanistic viewpoint. Antimicrobial Resistance & Infection Control 2019, 8(1): 1–28, 2019.
doi: 10.1186/S13756-019-0559-6
Koehn FE, Carter GT. The evolving role of natural products in drug discovery. Nature Reviews Drug Discovery, 4(3): 206–220, 2005.
doi: 10.1038/nrd1657
Laborda P, Sanz-García F, Hernando-Amado S, Martínez JL. Pseudomonas aeruginosa: an antibiotic resilient pathogen with environmental origin. Current Opinion in Microbiology, 64: 125–132, 2021.
doi: 10.1016/J.MIB.2021.09.010
Lee J, Zhang L. The hierarchy quorum sensing network in Pseudomonas aeruginosa. Protein & Cell, 6(1): 26–41, 2015.
doi: 10.1007/S13238-014-0100-X
Mielko KA, Jabłoński SJ, Milczewska J, Sands D, Łukaszewicz M, Młynarz P. Metabolomic studies of Pseudomonas aeruginosa. World Journal of Microbiology and Biotechnology, 35(11): 1–11, 2019.
doi: 10.1007/S11274-019-2739-1/FIGURES/2
Naz R, Lough M, Barthelmess E. Curcumin: a novel non-steroidal contraceptive with antimicrobial properties. Front Biosci, 8(1): 113–128, 2016.
doi: 10.2741/E755
Parai D, Banerjee M, Dey P, Chakraborty A, Islam E, Mukherjee SK. Effect of reserpine on Pseudomonas aeruginosa quorum sensing mediated virulence factors and biofilm formation. Biofouling, 34(3): 320–334, 2018.
doi: 10.1080/08927014.2018.1437910
Reeta K, Brijesh R, Anita R, Sonal B. Rauvolfia serpentina L. Benth. ex Kurz.: Phytochemical, Pharmacological and Therapeutic Aspects. International Journal of Pharmaceutical Sciences Review and Research, 23(2): 348–355, 2013.
Savli H, Karadenizli A, Kolayli F, Gundes S, Ozbek U, Vahaboglu H. Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR. Journal of Medical Microbiology,
52(5): 403–408, 2003.
doi: 10.1099/jmm.0.05132-0
Thorn RMS, Nelson SM, Greenman J. Use of a Bioluminescent Pseudomonas aeruginosa Strain within an In Vitro Microbiological System, as a Model of Wound Infection, To Assess the Antimicrobial Efficacy of Wound Dressings by Monitoring Light Production. Antimicrobial Agents and Chemotherapy, 51(9): 3217–3224, 2007.
doi: 10.1128/AAC.00302-07
Tyagi P, Singh M, Kumari H, Kumari A, Mukhopadhyay K. Bactericidal activity of curcumin I is associated with damaging of bacterial membrane. PLoS ONE, 10(3): 1–151, 2015.
doi:10.1371/journal.pone.0121313
How to Cite
Rúgeles, D., Gámez-Castillo, B., Gómez, V., & Hernández Rodríguez, P. (2022). Effects of natural compounds and commercial antibiotics on Pseudomonas aeruginosa quorum sensing: ingles. Universitas Scientiarum, 27(3), 357–372. https://doi.org/10.11144/Javeriana.SC273.eonc
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Clinical Microbiology
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