Keywords
Peroxidase
Hydrogen peroxide
Electrochemical detection
Biosensing
Screen-printed electrodes
How to Cite
Abstract
The Mimosa pudica, commonly known as the "sleepy plant," is renowned for its rapid leaf-folding response to touch, embodying an intriguing blend of dormancy and activity. Inspired by this unique behavior, we have developed a novel electrochemical sensor using screen-printed gold nanoparticle electrodes (SPGNPE) tretaed with Mimosa pudica peroxidase (MPP) for hydrogen peroxide determination (H₂O₂). The MPP exhibit a specific activity of 122.9 U/mg and operate optimally at pH 4.0 and a temperature of 55°C, indicating their robust performance under mildly acidic and moderately high-temperature conditions. The enzyme's inactivation rate constant (kinact) was determined to be -0.018 min-1, suggesting a lnstable enzymatic activity over time. Cyclic voltammetry (CV) experiments using potassium ferrocyanide as a redox probe demonstrated a significant increasing in current signal in the presence of MPP, indicating effective interaction of electrons with the redox compounds and the electrode interface. The linear relationship between the square root of the scan rate and the anodic and cathodic peaks, suggesting a surface controlled, semi-reversible process involving the migration of the electrochemically active species to the electrode interface. A low limit of detection (LOD) of 0.4 µM was achieved, accompanied by a sensitivity of 0.039 µA/µM, demonstrating the electrode's capability for precise and sensitive H₂O₂ quantification. This study highlights the unique application of the "sleepy plant" peroxidase, revealing its potential as a robust and sensitive novel bioelement in electrochemical sensing platforms. The synergy between nanomaterials and biological catalysts opens new avenues for environmentally-friendly and efficient detection systems.
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