ANÁLISIS GENÓMICO DE PARVOVIRUS CANINO POR PCR - RFLP A PARTIR DE AISLAMIENTOS DE CASOS CLÍNICOS SINTOMÁTICOS TOMADOS EN BOGOTÁ, COLOMBIA. ESTUDIO PRELIMINAR
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Abstract
With the objective of detemúning the genotypes of canine parvovirus (CPV) circulating in Bogotá, 56 fecal and blood samples were obtained from younger dogs, up to a year old with hemorrhagic gastroenteritis for its analysis by the polymerase chain reaction - fingerprints (PCR - RFLP) assay. DNA fragments were obtained from (2,2 Kb) a representative vaccine strain of parvovirus type -2 (CPV-2) and from the four fecal samples were amplified by the PCR assay; they were not obtained from the amplification of the blood samples. The amplified products were digested by the restriction enzyme Rsa I, pemútting the identification of genotypes PCV-2a and PCV-2b and the differentiation of CPV-2 from CPV-2a and CPV-2b.
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How to Cite
Castillo, Ángela, Díez, H., Almanza, J., Jerabek, L., & Torres, O. (2013). ANÁLISIS GENÓMICO DE PARVOVIRUS CANINO POR PCR - RFLP A PARTIR DE AISLAMIENTOS DE CASOS CLÍNICOS SINTOMÁTICOS TOMADOS EN BOGOTÁ, COLOMBIA. ESTUDIO PRELIMINAR. Universitas Scientiarum, 6(2), 49–53. Retrieved from https://revistas.javeriana.edu.co/index.php/scientarium/article/view/4838
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Veterinary Microbiology