Anthocyanin Production Evaluation Using Plant Cell Cultures. Growth and Viability Analysis at Different Process Conditions

Carlos Andrés García Velásquez, Carlos Ariel Cardona Álzate

Abstract


The comparison between the structured and unstructured models was performed in order to determine the mathematical approach that best fit the biological synthesis of anthocyanins from plant cell cultures. A structured model was used for the production of secondary metabolites using cell cultures of strawberry (Fragaria ananassa) taking into account the cell viability of pigmented and non-pigmented cells. Moreover, growth and anthocyanin production of cell suspensions of grape (Vitis vinifera) were evaluated by an unstructured model. Both models adequately described the growth of plant cells and substrate uptake. However, anthocyanin production is intrinsically related to cell growth, especially degradation thereof; therefore the structured model was selected to describe the production of this secondary metabolite.


Keywords


anthocyanin; plant cell cultures; kinetic models

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DOI: http://dx.doi.org/10.11144/Javeriana.iyu20-1.apeu

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