Published Dec 7, 2015



PLUMX
Almetrics
 
Dimensions
 

Google Scholar
 
Search GoogleScholar


Carlos Andrés García-Velásquez, BSc

Carlos Ariel Cardona-Álzate, PhD

##plugins.themes.bootstrap3.article.details##

Abstract

The comparison between the structured and unstructured models was performed in order to determine the mathematical approach that best fit the biological synthesis of anthocyanins from plant cell cultures. A structured model was used for the production of secondary metabolites using cell cultures of strawberry (Fragaria ananassa) taking into account the cell viability of pigmented and non-pigmented cells. Moreover, growth and anthocyanin production of cell suspensions of grape (Vitis vinifera) were evaluated by an unstructured model. Both models adequately described the growth of plant cells and substrate uptake. However, anthocyanin production is intrinsically related to cell growth, especially degradation thereof; therefore the structured model was selected to describe the production of this secondary metabolite.

Keywords

anthocyanin, plant cell cultures, kinetic modelsAntocianinas, Cultivos Celulares Vegetales, Modelos cinéticos

References
[1] S. Oancea, M. Stoia, and D. Coman, “Effects of extraction conditions on bioactive anthocyanin content of Vaccinium corymbosum in the perspective of food applications,” Procedia Eng., vol. 42, no. August, pp. 489–495, 2012.
[2] G. Wong, Química de los alimentos: mecanismos y teoría. Madrid: Edición A., 1995.
[3] W. Zhang, M. Seki, S. Furusaki, and A. Middelberg, “Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells,” J. Ferment. Bioeng., vol. 86, no. 1, pp. 72–78, 1998.
[4] D.K. Dougall and K.W. Weyrauch, “Growth and anthocyanin production by carrot suspension cultures grown under chemostat conditions with phosphate as the limiting nutrient,” Biotechnol. Bioeng., vol. 22, no. 2, pp. 337–352, 1980.
[5] T.J. Hirasuna, M.L. Shuler, V.K. Lackney, and R.M. Spanswick, “Enhanced anthocyanin production in grape cell cultures,” Plant Sci., vol. 78, no. 1, pp. 107–120, 1991.
[6] W. Zhang, S. Furusaki, and C. Franco, “A two-stage process of temperature-shift for enhanced anthocyanin production in strawberry cell suspension cultures,” Sci. China, pp. 345–350, 1999.
[7] J.-J. Zhong and T. Yoshida, “High density cultivation of Perilla frutescens cell suspensions for anthocyanin production: Effects of sucrose concentration and inoculum size,” Enzyme Microb. Technol., vol. 17, no. 12, pp. 1073–1079, 1995.
[8] J. Guardiola, J.L. Iborra, M. Canovas, and M. Cánovas, “A model that links growth and secondary metabolite production in plant cell suspension cultures,” Biotechnol. Bioeng., vol. 46, no. 3, pp. 291–297, 1995.
[9] D. Drapeau, H. Blanch, and C. Wike, “Growth kinetics of Dioscorea deltoidea and Catharanthus roseus in batch cultures,” Biotechnol. Bioeng., pp. 291–297, 1994.
[10] J. Monod, “The growth of bacterial cultures,” Annu. Rev. Microbiol., vol. 3, p. 371, 1947.
[11] C. Bailey and H. Nicholson, “A new structured model for plant cell culture,” Biotechnol. Bioeng., pp. 1331–1336, 1989.
[12] W. Zhang and S. Furusaki, “Production of anthocyanins by plant cell cultures,” Biotechnol. Bioprocess Eng., vol. 4, no. 4, pp. 231–252, 1999.
[13] W. W. Su and F. Lei, “Rosmarinic acid production in Perfused Anchusa Officinalis culture: Effect of inoculum size,” Biotechnol. Lett., vol. 15, no. 10, pp. 1035–1038, 1993.
[14] G. Patil and K. S. M. S. Raghavarao, “Integrated membrane process for the concentration of anthocyanin,” J. Food Eng., vol. 78, no. December 2005, pp. 1233–1239, 2007.
[15] J. Moncada, M.M. El-Halwagi, and C.A. Cardona, “Techno-economic analysis for a sugarcane biorefinery: Colombian case,” Bioresour. Technol., vol. 135, pp. 533–543, 2013.
[16] Food and Agriculture Organization of the United States (FAO), “Sugar crops and sweeteners and derived products.” [Online]. Available: www.fao.org. [Accessed: 05-Jul-2015].
How to Cite
García-Velásquez, C. A., & Cardona-Álzate, C. A. (2015). Anthocyanin production evaluation using plant cell cultures. growth and viability analysis at different process conditions. Ingenieria Y Universidad, 20(1), 7–20. https://doi.org/10.11144/Javeriana.iyu20-1.apeu
Section
Bioengineering and chemical engineering

Most read articles by the same author(s)