Published Jul 12, 2022


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John Castillo, PhD

Paula Andrea Guarin-Guio

Ludy Ortiz



Objective: In this article a comparison was made between graphene (SPGE) and graphene oxide screen-printed electrodes (SPGOE) to study the bio-electrocatalytic reduction of hydrogen peroxide (H2O2) by guinea grass peroxidase (GGP). Methods and materials: GGP was immobilized onto SPGE and SPGOE by a drop-casting procedure. Electrochemical techniques were carried out to monitor the electrochemical behavior of GGP and the efficiency of electrocatalytic reduction of H2O2. Results and discussion: GGP adsorbed on both electrodes exhibited a couple of well-defined redox peaks at 120 mV/10.5 mV and 184 mV/59 mV for anodic and cathodic peaks, respectively. Linearity between scan rates root and oxidation and reduction peak currents for both electrodes suggest a surface-controlled process. The GGP-modified electrodes exhibited a good electrocatalytic activity to H2O2 reduction at a redox potential of -0.6 V and -0.5 V for SPEG and SPEGO, respectively. Conclusions: SPGE and SPGOE electrodes modified with GGP showed excellent analytical performance towards different concentrations of hydrogen peroxide. This is a preliminary step to developing a bio-analytical portable system based on GGP for the detection of H2O2 in real environmental samples.


Peroxidase, guinea grass, hydrogen peroxide, screen-printed electrode, graphene, graphene oxidePeroxidasa, pasto guinea, peróxido de hidrógeno, electrodos serigrafiados, grafeno, óxido de grafeno

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How to Cite
Castillo, J., Guarin-Guio, P. A., & Ortiz, L. (2022). Bio-Electrocatalytic Reduction of Hydrogen Peroxide by Peroxidase from Guinea Grass (Panicum Maximum) Immobilized on Graphene and Graphene Oxide Screen-Printed Electrodes. Ingenieria Y Universidad, 26.
Bioengineering and chemical engineering