Palabras clave
pasto guinea
peróxido de hidrógeno
electrodos serigrafiados
grafeno
óxido de grafeno
Cómo citar
Resumen
Objetivo: En este artículo se llevó a cabo una comparación entre electrodos serigrafiados de grafeno (ESG) y óxido de grafeno (ESOG) para el estudio de la bio-reducción electrocatalítica de peróxido de hidrógeno (H2O2) usando la peroxidasa de pasto guinea (PPG). Materiales y métodos: La PPG fue inmovilizada en ESG y ESOG mediante un procedimiento sencillo de adsorción. El estudio del comportamiento electroquímico de la PPG y la eficiencia en la reducción electrocatalítica de H2O2 fue estudiada mediante técnicas electroquímicas. Resultados y discusión: La PPG adsorbida sobre los dos electrodos mostró una pareja de señales redox bien definidas a 120 mV/10.5 mV y 184 mV/59 mV para los picos anódicos y catódicos respectivamente. La linealidad existente entre la raíz cuadrada de la velocidad de barrido y las corrientes de oxidación y reducción sugieren la existencia de un proceso controlado por difusión superficial. Los electrodos modificados con la GGP mostraron una buena actividad electrocatalítica en la reducción del H2O2 con potenciales redox en -0.6 y -0.5 V para ESG y ESOG, respectivamente. Conclusión: Los electrodos ESG y ESOG modificados con la PPR exhibieron un excelente desempeño analítico hacia diferentes concentraciones de peróxido de hidrógeno siendo esto un paso preliminar en el desarrollo de novedosos sistemas bio-analíticos portables usando la PPG para la detección de H2O2 en muestras de interés ambiental o biomédico.
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