Expression of Proliferation Cell Nuclear Antigen (PCNA) in Odontogenic Keratocysts and Dentigerous Cysts
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BACKGROUND: Proliferation Cell Nuclear Antigen (PCNA), marker used in studies as a prognostic tool, is observed in different lesions with a high percentage. That suggests that PCNA could be used to evaluate tissular biological activity and to predict malignancy. OBJECTIVE: To compare the expression of PCNA in dentigerous cysts and keratocysts to predict neoplastic activity. METHODS: An observational analytic study was carried out. The sample consisted of 30 4-μm cuts of biopsies that were stained through hematoxylin-eosin to identify the cystic epithelium. Later, the immunohistochemical technique was carried out to identify PCNA positive cells; with these cells it was pretended to see any cell proliferation. RESULTS: Dentigerous cysts showed positive PCNA stain at the stratum level of the basal cells, and negative for the suprabasal cells. Orthokeratoscysts showed positive stain for PCNA in the suprabasal cells and negative for the stratum of basal cells. This same result was seen in all 10 parakeratocyst cases. The parakeratocysts showed satellite cells and also the highest number of PCNA positive cells, which means that this cyst was the most aggressive. There was no significant statistic difference between the orthokeratocyst and the parakeratocyst, but there was a big difference between these two and the dentigerous cyst (p<0.05). CONCLUSIONS: PCNA expression in suprabasal cells of the orthokeratocyst or parakeratocyst shows the autonomous role of such cells to proliferate. The parakeratocyst proved to be the most aggressive cyst.
antígeno nuclear de proliferación celular, PCNA, quiste dentígero, quiste ortoqueratósico, quiste paraqueratósico, patología oralProliferation Cell Nuclear Antigen, PCNA, dentigerous cyst, orthokeratocyst, parakeratocyst, Oral Pathology
2. Regezi J. Patología Bucal. Primera edición, México DF, México: Interamericana McGraw-Hill, 1989; 328-9
3. Stenman G, Magnusson B. In vitro growth characteristics of human odontogenic keratocyst and dentigerous cysts. J Oral Pathol 1986 Mar; 15(3): 143-5
4. Copete MA, Cleveland DB, Orban RE Jr., Chen SY. Squamous carcinoma arising from a dentigerous cyst: Report of a case. Compend Contin Educ Dent 1996 Feb; 17(2): 202-4
5. Roofe SB, Boyd EM Jr., Houston GD, Edgin WA. Squamous cell carcinoma arising in the epithelia lining of a dentigerous cyst. South Med J 1992 Jun; 92(6): 611-4
6. McDonald AR, Pogrel MA, Carson J, Regezi J. p53 positive squamous cell carcinoma originating from a odontogenic cyst. J Oral Maxillofac Surg 1996 Feb; 54(2): 216-8
7. Stoelinga PJ. Long term follow-up on keratocysts treated according to a defined protocol. Int J Oral Maxillofac Surg 2001 Nov; 30(1): 14-25
8. Abraldes M, Capeans C. Estudio de la expresión de p53 en el melanoma maligno uveal. Arch Soc Esp Oftamol 1998 Jun; 9(32): 344-51
9. Riganti JG, Machuca M. Inmunomarcación de antígeno nuclear de proliferación celular y de apoptosis en íleon de cerdos con enteropatía proliferativa. J Ciencias Veterinarias 2000 Feb; 12(3):42
10. Waga S, Stillman B. Anatomy of DNA replication for revealed by reconstitution of SV40 DNA replication in vitro. Nature 1994 May; 19(6477): 207-12
11. Adelsperger J, Campbell JH, Coates DB, Summerlin DJ, Tomich CE. Early soft pathosis associated with impacted third molars without pericoronal radiolucency. J Oral Surg Oral Med Oral Pathol Radiol Endod 2002 Apr; 89(4): 409-6
12. Ghazizadeh M, Sasaki Y, Araki T, Kinishi H, Aihara K. Prognostic value of proliferative activity of ovarian carcinoma as revealed by PCNA and AgNOR analyses. Am J Clin Pathol 1997 Apr; 107(4):451-8
13. Hamakawa H, Omori T, Sumida T, Tanioka H. Intraosseus epithelioid hemangioendothelioma of the mandible: A case report with an immunohistochemical study. J Oral Pathol Med 1999 May; 28(5): 233-7
14. Fraga M. Prothymosin alpha as proliferation marker in Hodgkin’s disease: Comparison with MIB1 and PCNA. J Soc Esp Citol 1997 May; 2(30): 121-6
15. Tsuji T, Shrestha P, Yamada K, Takagi H, Shinozaki F et al. Proliferating cell nuclear antigen in malignant and pre-malignant lesions of epithelial origin in the oral cavity and the skin: An immunohistochemical study. Virchows Arch A Pathol Anat Histopathol 1992; 420(5): 377-83
16. Bucci E, Mignogna M, Lo Muzio L. Ameloblastoma unicistico ed hiperplasia epiteliale plessiforme. Minerva Stomatol 1988 Jun; 37(6): 541-5
17. Canh LR. The dentigerous cyst is a potential adamantinoma. Dent Cosmos 1993 Oct; 75(16): 889
18. Li TJ, Browne RM, Matthews JB. Expression of proliferating cell nuclear antigen (PCNA) and Ki- 67 in unicystic ameloblastoma. Histopathology 1995 Mar; 26(3): 219-28
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