Published Aug 16, 2013



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Paola Andrea Ayala Ramírez

Liz Ariane Gonzalez Ropero

Reggie García Robles

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Abstract

The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified. Here we studied the effect of storage time (15 to 30 days) and temperature (4°C, -20°C and ambient) and of the presence of Trizol on the concentration of RNA in plasma samples taken from pregnant women before their 20th week. Thirty-three RNA samples were extracted from plasma and analyzed by spectrophotometry. The amount of RNA was statistically lower in samples stored at -20°C whereas storage time and the presence or absence of Trizol did not affect RNA level. Temperature affected the concentration of RNA, rather than time and the addition of Trizol. In conclusion, temperature is a key factor in the extraction of RNA, and the freeze-thaw process most likely affects RNA concentrations  negatively.

Keywords

Ribonucleic acid, plasma, pregnancy complicationsRibonucleic acid, plasma, pregnancy complications

References
How to Cite
Ayala Ramírez, P. A., Gonzalez Ropero, L. A., & García Robles, R. (2013). Temperature of storage and amount of RNA extracted from maternal plasma. Universitas Scientiarum, 18(2), 181–187. https://doi.org/10.11144/Javeriana.SC18-2.taca
Section
Biología molecular / Molecular biology / Biologia molecular