SIMPLE PROCEDURES FOR EXTRACTION OF TOTALAND CHLOROPLAST DNA FROM Passijlora SPECIES
PDF

Keywords

Chloroplast Isolation
DNA Isolation
Passionfruit
Passiflora edulis
vines

How to Cite

SIMPLE PROCEDURES FOR EXTRACTION OF TOTALAND CHLOROPLAST DNA FROM Passijlora SPECIES. (2013). Universitas Scientiarum, 3(1-2), 99-105. https://revistas.javeriana.edu.co/index.php/scientarium/article/view/5059
Almetrics
 
Dimensions
 

Google Scholar
 
Search GoogleScholar

Abstract

We describe simple protocols for the extraction of total DNA and ctDNA from Passiflora species. Total DNA was purified from less than 40mg of different tissue sources (buds, tendrils, stipules, nodes or leaves) in the presence ofPVPP to avoid polyphenols. ctDNA isolation was based on the lysis of chloroplasts purified by sucrose step gradients. Up to 50mg of ctDNA were obtained from less than 20g ofleaf tissue. Both procedures yielded clean DNA suitable for PCR amplification and restriction endonuclease digestion. These techniques can be used for the analysis of DNA from single plants using small amounts of tissu
PDF

Univ. Sci. is registered under a Creative Commons Attribution 4.0 International Public License. Thus, this work may be reproduced, distributed, and publicly shared in digital format, as long as the names of the authors and Pontificia Universidad Javeriana are acknowledged. Others are allowed to quote, adapt, transform, auto-archive, republish, and create based on this material, for any purpose (even commercial ones), provided the authorship is duly acknowledged, a link to the original work is provided, and it is specified if changes have been made. Pontificia Universidad Javeriana does not hold the rights of published works and the authors are solely responsible for the contents of their works; they keep the moral, intellectual, privacy, and publicity rights. Approving the intervention of the work (review, copy-editing, translation, layout) and the following outreach, are granted through an use license and not through an assignment of rights. This means the journal and Pontificia Universidad Javeriana cannot be held responsible for any ethical malpractice by the authors. As a consequence of the protection granted by the use license, the journal is not required to publish recantations or modify information already published, unless the errata stems from the editorial management process. Publishing contents in this journal does not generate royalties for contributors.