Vol. 3 No. 1-2 (1996), Biotechnology
Vol. 3 No. 1-2 (1996)

SIMPLE PROCEDURES FOR EXTRACTION OF TOTALAND CHLOROPLAST DNA FROM Passijlora SPECIES

Biotechnology
Gustavo Góngora F.
Unidad de Biología Vegetal, Departamento de Biología, Pontificia Universidad Javeriana, Bogotá,
Elizabeth Hodson de Jaramillo
Unidad de Biología Vegetal, Departamento de Biología, Pontificia Universidad Javeriana, Bogotá,
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Keywords

Chloroplast Isolation
DNA Isolation
Passionfruit
Passiflora edulis
vines

How to Cite

SIMPLE PROCEDURES FOR EXTRACTION OF TOTALAND CHLOROPLAST DNA FROM Passijlora SPECIES. (2013). Universitas Scientiarum, 3(1-2), 99-105. https://revistas.javeriana.edu.co/index.php/scientarium/article/view/5059
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Abstract

We describe simple protocols for the extraction of total DNA and ctDNA from Passiflora species. Total DNA was purified from less than 40mg of different tissue sources (buds, tendrils, stipules, nodes or leaves) in the presence ofPVPP to avoid polyphenols. ctDNA isolation was based on the lysis of chloroplasts purified by sucrose step gradients. Up to 50mg of ctDNA were obtained from less than 20g ofleaf tissue. Both procedures yielded clean DNA suitable for PCR amplification and restriction endonuclease digestion. These techniques can be used for the analysis of DNA from single plants using small amounts of tissu
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