Evaluation of Possible DNA Damage in Oral Mucosa Cells Using the Micronucleus Test in Pediatric Patients with Removable Appliances
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Background: Orthodontic and orthopedic materials are made of stainless steel, with chemical elements such as nickel and chromium, generally associated with allergenic effects and hypersensitivity, expressed locally. Purpose: To examine, using the micronucleus test in oral mucosa cells, DNA damage in patients undergoing removable metallic orthopedics. Methods: Samples were collected before the appliance adaptation and 30 days later, the buccal cells of each individual were collected by gently brushing the internal part of the cheeks with a cytological brush. The plates were immersed in a Coplin jar with 0.2 % Light Green solution for 1 minute, and then were rinsed with deionized water or Milli Q to remove excess dye. Later, these were placed on a drying plate between 50ºC and 80ºC for 10 to 15 minutes. Results: Compared with the initial samples, after 1 month of orthopedic treatment, a significant increase in the frequency of cells with micronuclei, nuclear budding and karyorrhexis was observed. No changes or evidence of an increase were observed in cells with condensed chromatin. Conclusions: The stages of nuclear alteration that took place during the development of this study were MN frequency, nuclear budding and karyorrhexis, registering a higher frequency in the second sampling and attributed to the presence of removable appliances in the patients. Pyknosis, karyolysis, and karyorrhexis do not constitute stages of nuclear alteration, the latter being the one with the greatest trend observed in the first and second sampling. For this stage, a significant increase in number was found, attributed to the presence of removable appliances in the patients.
cytotoxicity, dental orthopedics, dentistry, genotoxicity, micronucleus test, oral mucosa, oral pathology, orthodontic appliance, saliva, toxicityaparatos ortodónticos, citotoxicidad, genotoxicidad, mucosa oral, odontologia, ortopedia dentofacial, patología oral, prueba de micronúcleos, saliva, toxicidadaparelhos ortodônticos, citotoxicidade, genotoxicidade, mucosa bucal, odontologia, ortopedia dentofacial, patologia oral, saliva, teste de micronúcleo, toxicidade
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