Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia English
Published
Dec 22, 2020
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Deisy Lorena Guerrero Ceballos
Edith Mariela Burbano Rosero
https://orcid.org/0000-0002-4021-2660
Eduardo Ibargüen-Mondragon
https://orcid.org/0000-0001-6308-1344
https://orcid.org/0000-0002-4021-2660
Eduardo Ibargüen-Mondragon
https://orcid.org/0000-0001-6308-1344
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Abstract
Bacterial resistance to antibiotics is one of the largest medical concerns worldwide.One of the bacteria of critical priority is E. coli, since it presents different resistance mechanisms and some of its strains have evolved resistance to beta-lactam antibiotics. We characterized 32 antibiotic resistant bacterial isolates from confirmed cases of urinary tract infections from an array of patients in Nariño, southern Colombia. Macro and microscopic descriptions of the 32 clinical isolates were conducted. Resistance profiles, biochemical, and molecular characterization (via 16S rRNA gene sequencing, ERIC-PCR, and resistance genes) were performed. All the isolates were identified as E. coli and had resistance to beta-lactams, aminoglycosides, and fluoroquinolones. This resistance was related to plasmids carryingthebla TEM, blaSHV1, and blaCTXM1genes.There were significant differences between the resistance proportions of the samples (p value:0.0000), mainly to penicillin, cefotoxin, and imipenem. Using ERIC-PCR, four clonal states were evidenced that corroborate a degree of genetic differentiation with in the isolate set.Antibiotic resistance observed in the isolates is associated with resistance genes present in the bacterial chromosome and plasmids.
Keywords
Englishantibiotics, Escherichiacoli, urinary infections, plasmids and resistance
References
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characterization of Escherichia coli strains isolated from healthy adults in Ho Chi Minh City, Vietnam, Journal of Veterinary Medical Science, 79: 479-485, 2017.
doi: 10.1292/jvms.16-0639
[23] Guzman M, Salazar E, Cordero V, Castro A, Villanueva A, Rodulfo, H. Multidrug resistance and risk factors associated
to community-acquired urinary tract infections caused by Escherichia coli in Venezuela, Biomédica, 39: 96-106, 2019.
doi: 10.7705/biomedica.v39i2.4030
[24] Abril D, Bustos Moya I, Marquez-Ortiz R, Josa Montero D, Corredor Rozo Z, Torres Molina I, Escobar-Perez J. First Report and Comparative Genomics Analysis of a blaOXA-244-Harboring Escherichia coli Isolate Recovered in the American Continent, Antibiotics, 8: 222, 2019.
doi: 10.3390/antibiotics8040222
[25] Martínez D, Araque Y, Rodulfo H, Caña L, García J and Gonzáles D. Relación clonal y detección del gen blaKPC en cepas de Klebsiella pneumoniae resistentes a carbapenémicos, en un hospital de Venezuela, Revista Chilena Infectologia, 33: 524-530, 2016.
doi: 10.4067/S0716-10182016000500006
[2] Singh AK, Das S, Singh S, Gajamer VR, Pradhan N, Lepcha YD and Tiwari HK Prevalence of antibiotic resistance in commensal Escherichia coli among the children in rural hill communities of Northeast India, PloS one, 13: e0199179, 2018.
doi: 10.1371/journal.pone.0199179
[3] Organización Mundial de la Salud (OMS). Informe Anual de la Red de Monitoreo: Vigilancia de la Resistencia a los Antibióticos. Asociación Brasilera de editores cientificos 2015.
[4] Aguinaga A, Gil-Setas A, Ramos AM, Alvaro A, García-Irure JJ, Navascués A, and Ezpeleta Baquedano C. Infecciones del tracto urinario estudio de sensibilidad antimicrobiana en Navarra, In Anales del sistema sanitario de Navarra, 41: 17-26, 2018.
doi: 10.23938/assn.0125
[5] Alviz-Amador A, Gamero-Tafur K, Caraballo-Marimon R and Gamero-Tafur J. Prevalencia de infección del tracto urinario, uropatógenos y perfil de susceptibilidad en un hospital de Cartagena, Colombia 2016, Revista Facultad de Medicina, 66: 313-317, 2018.
doi: 10.15446/revfacmed.v66n3.62601
[6] Hospital Departamental de Nariño. Boletin epidemiológico periodo enero-septiembre 2015. Pasto- Nariño, Colombia, 2018.
[7] CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 28th ed. CLSI supplement M100.Wayne, PA: Clinical and Laboratory Standards Institute 2018.
[8] Burbano- Rosero M, Caetano de Almeida B, Otero-Ramírez I. Manual de Biología Molecular- Procedimientos Básicos, Manual de Biología Molecular- Procedimientos Básicos, Pasto, Colombia, 2017:
13-50, 2017.
[9] Arce-Gil Z, Llontop-Nuñez J, Flores- Clavo R, Fernández Valverde D. Detección del gen CTX-M en cepas de Escherichia coli
productoras de β-lactamasas de espectro extendido procedentes del Hospital Regional de Lambayeque, Revista del Cuerpo Médico del HNAAA, 6: 13-16, 2013.
[9] Weisburg WG, Barns SM, Pelletier DA, Lane DJ.16S ribosomal DNA amplification for phylogenetic study, Journal of bacteriology, 173: 697-703, 1991.
doi: 10.1128/jb.173.2.697-703.1991
[10] Nübel U, Engelen B, Felske A, Snaidr J, Wieshuber A, Amann RI. Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis, Journal of bacteriology, 178: 5636-5643, 1996.
doi: 10.1128/jb.178.19.5636-5643.1996
[11] Adiguzel A, Ozkan H, Baris, O. Inan K, Gulluce M, Sahin F. Identification and characterization of thermophilic bacteria
isolated from hot springs in Turkey, Journal of microbiological methods, 79: 321-328, 2009.
doi: 10.1016/j.mimet.2009.09.026
[12] Browning DF, Busby SJ. Local and global regulation of transcription initiation in bacteria. Nature Reviews Microbiology,
14(10), 638, 2016.
doi: 10.1038/nrmicro.2016.103
[13] Bergey DH, Holt JG. Bergey’s manual of determinative bacteriology (Novena ed.). Maryland, EUA: Lippicontt Williams and Wilkins. 1994.
[14] Yábar MN, Curi-Pesantes B, Torres CA, Calderón-Anyosa R, Riveros M, Ochoa TJ. Multirresistencia y factores asociados a la presencia de betalactamasas de espectro extendido en cepas de Escherichia coli provenientes de urocultivos, Revista Peruana de Medicina Experimental y Salud Pública, 34: 660-665, 2017.
doi: 10.17843/rpmesp.2017.344.2922
[15] Luna-Pineda VM, Ochoa SA, Cruz-Córdova A, Cázares-Domínguez V, Reyes-Grajeda JP, Flores-Oropeza MA. Features of
urinary Escherichia coli isolated from children with complicated and uncomplicated urinary tract infections in Mexico, PloS one, 13: e0204934, 2018.
doi: 10.1371/journal.pone.0204934
[16] Nocua-Báez LC, Cortés JA, Leal AL, Arias GF, Ovalle-Guerrero} MV, Saavedra-Rojas SY. Perfil de sensibilidad antimicrobiana de microorganismos causantes de infecciones urinarias adquiridas en la comunidad en pacientes con diabetes mellitus en Colombia, Biomédica, 37: 453-460, 2017.
doi: 10.7705/biomedica.v37i3.3348
[17] Mahalingam N, Manivannan B, Khamari B, Siddaramappa S, Adak S, Bulagonda EP. 2018. Detection of Antibiotic Resistance Determinants and their Transmissibility among Clinicallyisolated Carbapenem-resistant Escherichia coli from South India, Medical Principles and Practice, act 2018: 428-435, 2018.
doi: 10.1159/000489885
[18] Bryce A, Hay AD, Lane IF, Thornton HV, Wootton M, Costelloe C. Global prevalence of antibiotic resistance in paediatric urinary tract infections caused by Escherichia coli and association with routine use of antibiotics in primary care: systematic review and meta-analysis, bmj, 2016: 352-i939, 2016.
doi: 10.1136/bmj.i939
[19] Rada AM, Hernández-Gómez C, Restrepo E, Villegas M. Distribución y caracterización molecular de beta-lactamasas en
bacterias Gram negativas en Colombia (2001-2016), Biomédica, 39: 199-220, 2019.
doi: 10.7705/biomedica.v39i3.4351
[20] Argente M, Miró E, Martí C, Vilamala A, Alonso-Tarrés C, Ballester F. 2019. Molecular characterization of OXA-48
carbapenemase-producing Klebsiella pneumoniae strains after a carbapenem resistance increase in Catalonia, Enfermedades infecciosas y Microbiologia clínica, 37: 82-88, 2019.
doi: 10.1016/j.eimc.2018.02.003
[21] Jamborova I, Dolejska M, Zurek L, Townsend AK, Clark AB, Ellis JC. Plasmid-mediated resistance to cephalosporins and
quinolones in Escherichia coli from American crows in the USA, Environmental Microbiology, 19: 2025-2036, 2017.
doi: 10.1111/1462-2920.13722
[22] Hoang PH, Awasthi SP, Do Nguyen P, Nguyen NL, Nguyen DT, Le NH. Antimicrobial resistance profiles and molecular
characterization of Escherichia coli strains isolated from healthy adults in Ho Chi Minh City, Vietnam, Journal of Veterinary Medical Science, 79: 479-485, 2017.
doi: 10.1292/jvms.16-0639
[23] Guzman M, Salazar E, Cordero V, Castro A, Villanueva A, Rodulfo, H. Multidrug resistance and risk factors associated
to community-acquired urinary tract infections caused by Escherichia coli in Venezuela, Biomédica, 39: 96-106, 2019.
doi: 10.7705/biomedica.v39i2.4030
[24] Abril D, Bustos Moya I, Marquez-Ortiz R, Josa Montero D, Corredor Rozo Z, Torres Molina I, Escobar-Perez J. First Report and Comparative Genomics Analysis of a blaOXA-244-Harboring Escherichia coli Isolate Recovered in the American Continent, Antibiotics, 8: 222, 2019.
doi: 10.3390/antibiotics8040222
[25] Martínez D, Araque Y, Rodulfo H, Caña L, García J and Gonzáles D. Relación clonal y detección del gen blaKPC en cepas de Klebsiella pneumoniae resistentes a carbapenémicos, en un hospital de Venezuela, Revista Chilena Infectologia, 33: 524-530, 2016.
doi: 10.4067/S0716-10182016000500006
How to Cite
Guerrero Ceballos, D. L., Burbano Rosero, E. M., & Ibargüen-Mondragon, E. (2020). Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia: English. Universitas Scientiarum, 25(3), 463–488. https://doi.org/10.11144/Javeriana.SC25-3.coar
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Section
Microbiología / Microbiology / Microbiologia
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