Modificaciones en la plasticidad hipocampal a corto plazo por efecto de un péptido diseñado y sintetizado con base en la estructura de una conotoxina
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sep 4, 2012
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Resumen
Introducción: El diseño de instrumentos o moléculas inspiradas en la naturaleza ha permitido avanzar en diferentes áreas de la ciencia y tecnología.
Objetivo: Evaluar cambios plásticos a corto plazo desencadenados por la administración intraperitoneal de un péptido agonista del receptor de glutamato NMDA.
Materiales y métodos: Se utilizaron ratas macho divididas en grupo péptido (a quienes se administró peritonealmente el péptido) y grupo control (a quienes se administró peritonealmente el vehículo). Por técnicas de estereotaxia se localizaron electrodos de estimulación y registro en las áreas hipocampales CA3 y CA1 respectivamente, con el ánimo de obtener los potenciales de campo y analizar cambios en su pendiente y amplitud por la aplicación de pares de estímulos, antes y después de la administración del péptido.
Resultados: Aunque con la administración del péptido no hay cambios en variables sistémicas como la temperatura o la frecuencia cardiaca, sí se pueden modificar los potenciales de campo, específicamente cuando el intervalo entre pares de estímulos es más corto (40 a 80 ms). Conclusión: El péptido evaluado en el presente trabajo tiene efectos en la facilitación por pulsos pareados tanto en la amplitud como en la pendiente de potenciales de campo en el hipocampo de ratas, pero en los intervalos entre estímulos más cortos.
Keywords
conotoxinas, toxinología, hipocampo, Conotoxins, toxinology, hippocampus,
References
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33. Buonanno A. Presynaptic NMDA receptors also make the switch. Nat Neurosci. 2011;14:274-6.
34. McGuinness L, Taylor C, Taylor RD, Yau C, Langenhan T, Hart ML et al. Presynaptic NMDARs in the hippocampus facilitate transmitter release at theta frequency. Neuron. 2010;68:1109-27.
35. Decker H, Jurgensen S, Adrover MF, Brito-Moreira J, Bomfim TR, Klein WL et al. N-methyl-D-aspartate receptors are required for synaptic targeting of Alzheimer’s toxic amyloid-beta peptide oligomers. J Neurochem. 2010;115:1520-9.
36. Kurup P, Zhang Y, Xu J, Venkitaramani DV, Haroutunian V, Greengard P et al. Abeta-mediated NMDA receptor endocytosis
in Alzheimer’s disease involves ubiquitination of the tyrosine phosphatase STEP61. J Neurosci. 2010;30:5948-57.
37. Cranwell-Bruce LA. Drugs for Alzheimer’s disease. Medsurg Nurs. 2010;19:51-3.
38. Koutsilieri E, Riederer P. Excitotoxicity and new antiglutamatergic strategies in Parkinson’s disease and Alzheimer’s disease. Parkinsonism Relat Disord. 2007;13 Suppl 3:S329-S331.
2. Petrenko AB, Yamakura T, Baba H, Shimoji K. The role of N-methyl-D-aspartate (NMDA) receptors in pain: a review. Anesth Analg. 2003;97:1108-16.
3. South SM, Kohno T, Kaspar BK, Hegarty D, Vissel B, Drake CT et al. A conditional deletion of the NR1 subunit of the NMDA receptor in adult spinal cord dorsal horn reduces NMDA currents and injury-induced pain. J Neurosci. 2003;23:5031-40.
4. Inturrisi CE. The role of N-methyl-Daspartate (NMDA) receptors in pain and morphine tolerance. Minerva Anestesiol. 2005;71:401-3.
5. Hingne PM, Sluka KA. Blockade of NMDA receptors prevents analgesic tolerance to repeated transcutaneous electrical nerve stimulation (TENS) in rats. J Pain. 2008;9:217-25.
6. Nazarian A, Gu G, Gracias NG, Wilkinson K, Hua XY, Vasko MR et al.
Spinal N-methyl-D-aspartate receptors and nociception-evoked release of primary afferent substance P. Neuroscience. 2008;152:119-27.
7. Tandon OP, Mehta AK, Halder S, Khanna N, Sharma KK. Peripheral interaction of opioid and NMDA receptors in inflammatory pain in rats. Indian J Physiol Pharmacol. 2010;54:21-31.
8. Alexander JK, DeVries AC, Kigerl KA, Dahlman JM, Popovich PG. Stress exacerbates neuropathic pain via glucocorticoid and NMDA receptor activation. Brain Behav Immun. 2009;23:851-60.
9. Jung YH, Suh YH. Differential functions of NR2A and NR2B in short-term and long-term memory in rats. Neuroreport. 2010;21:808-11.
10. Wang D, Cui Z, Zeng Q, Kuang H, Wang LP, Tsien JZ et al. Genetic enhancement of memory and long-term potentiation but
not CA1 long-term depression in NR2B transgenic rats. PLoS One. 2009;4:e7486.
11. von EJ, Doganci B, Jensen V, Hvalby O, Gongrich C, Taylor A et al. Contribution of hippocampal and extra-hippocampal NR2B-containing NMDA receptors to performance on spatial learning tasks. Neuron. 2008;60:846-60.
12. Hu M, Sun YJ, Zhou QG, Chen L, Hu Y, Luo CX et al. Negative regulation of neurogenesis and spatial memory by NR2B-containing NMDA receptors. J Neurochem. 2008;106:1900-13.
13. Jiao J, Nakajima A, Janssen WG, Bindokas VP, Xiong X, Morrison JH et al. Expression of NR2B in cerebellar granule cells specifically facilitates effect of motor training on motor learning. PLoS One. 2008;3:e1684.
14. Cao X, Cui Z, Feng R, Tang YP, Qin Z, Mei B et al. Maintenance of superior learning and memory function in NR2B transgenic mice during ageing. Eur J Neurosci. 2007;25:1815-22.
15. Zhao MG, Toyoda H, Lee YS, Wu LJ, Ko SW, Zhang XH et al. Roles of NMDA NR2B subtype receptor in prefrontal long-term potentiation and contextual fear memory. Neuron. 2005;47:859-72.
16. Loftis JM, Janowsky A. The N-methyl-Daspartate receptor subunit NR2B: localization, functional properties, regulation, and clinical implications. Pharmacol Ther. 2003;97:55-85.
17. Oyuela R, Lareo L, Muñoz L, Morales L, Echeverry S, Uribe A et al. Efecto en el aprendizaje y la memoria espacial de un péptido sintético en ratas: estudio preliminar. Psicología desde el Caribe. 2004;13:1-14.
18. Tzingounis AV, Nicoll RA. Presynaptic NMDA receptors get into the act. Nat Neurosci. 2004;7:419-20.
19. Mukhamedyarov MA, Zefirov AL, Palotas A. Paired-pulse facilitation of transmitter release at different levels of extracellular calcium concentration. Neurochem Res. 2006;31:1055-58.
20. Craig S, Commins S. Interaction between paired-pulse facilitation and long-term potentiation in the projection from hippocampal area CA1 to the entorhinal cortex. Neurosci Res. 2005;53:140-6.
21. Riveros A. Registros in vivo de la facilitación por pulsos pareados y la potenciación a largo plazo en la región CA1 de la rata: descripción y prueba de una técnica para la evaluación de la plasticidad sináptica. Revista MED. 2005;13:75-85.
22. Akopian G, Walsh JP. Corticostriatal paired-pulse potentiation produced by voltage-dependent activation of NMDA receptors and L-type Ca(2+) channels. J Neurophysiol. 2002;87:157-65.
23. Mukhamedyarov MA, Grishin SN, Zefirov AL, Palotas A. The mechanisms of multi-component paired-pulse facilitation of neurotransmitter release at the frog neuromuscular junction. Pflugers Arch. 2009;458:563-70.
24. Kim J, Alger BE. Random response fluctuations lead to spurious paired-pulse facilitation. J Neurosci. 2001;21:9608-18.
25. Leung LS, Peloquin P, Canning KJ. Paired- pulse depression of excitatory postsynaptic current sinks in hippocampal CA1 in vivo. Hippocampus. 2008;18:1008-20.
26. Scullin CS, Wilson MC, Partridge LD. Developmental changes in presynaptic Ca(2 +) clearance kinetics and synaptic plasticity in mouse Schaffer collateral terminals. Eur J Neurosci. 2010;31:817-26.
27. Rusakov DA. Ca2+-dependent mechanisms of presynaptic control at central synapses. Neuroscientist. 2006;12:317-26.
28. Catterall WA, Few AP. Calcium channel regulation and presynaptic plasticity. Neuron. 2008;59:882-901.
29. Luccini E, Musante V, Neri E, Raiteri M, Pittaluga A. N-methyl-D-aspartate autoreceptors respond to low and high agonist concentrations by facilitating, respectively, exocytosis and carrier-mediated release of glutamate in rat hippocampus. J Neurosci Res. 2007;85:3657-65.
30. Bardoni R, Torsney C, Tong CK, Prandini M, MacDermott AB. Presynaptic NMDA receptors modulate glutamate release from primary sensory neurons in rat spinal cord dorsal horn. J Neurosci. 2004;24:2774-81.
31. Duguid IC, Smart TG. Retrograde activation of presynaptic NMDA receptors enhances GABA release at cerebellar interneuron-Purkinje cell synapses. Nat Neurosci. 2004;7:525-33.
32. Tzingounis AV, Nicoll RA. Presynaptic NMDA receptors get into the act. Nat Neurosci. 2004;7:419-20.
33. Buonanno A. Presynaptic NMDA receptors also make the switch. Nat Neurosci. 2011;14:274-6.
34. McGuinness L, Taylor C, Taylor RD, Yau C, Langenhan T, Hart ML et al. Presynaptic NMDARs in the hippocampus facilitate transmitter release at theta frequency. Neuron. 2010;68:1109-27.
35. Decker H, Jurgensen S, Adrover MF, Brito-Moreira J, Bomfim TR, Klein WL et al. N-methyl-D-aspartate receptors are required for synaptic targeting of Alzheimer’s toxic amyloid-beta peptide oligomers. J Neurochem. 2010;115:1520-9.
36. Kurup P, Zhang Y, Xu J, Venkitaramani DV, Haroutunian V, Greengard P et al. Abeta-mediated NMDA receptor endocytosis
in Alzheimer’s disease involves ubiquitination of the tyrosine phosphatase STEP61. J Neurosci. 2010;30:5948-57.
37. Cranwell-Bruce LA. Drugs for Alzheimer’s disease. Medsurg Nurs. 2010;19:51-3.
38. Koutsilieri E, Riederer P. Excitotoxicity and new antiglutamatergic strategies in Parkinson’s disease and Alzheimer’s disease. Parkinsonism Relat Disord. 2007;13 Suppl 3:S329-S331.
Cómo citar
Riveros Rivera, A., & Guerrero, A. (2012). Modificaciones en la plasticidad hipocampal a corto plazo por efecto de un péptido diseñado y sintetizado con base en la estructura de una conotoxina. Universitas Medica, 54(1), 10–25. https://doi.org/10.11144/Javeriana.umed54-1.mphc
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