The placenta and fetal membranes have recently been proposed as an important stem cells source for regenerative medicine. Stem cells derived from amniotic membrane offer considerable advantages because of the ease of collection, their low immunogenicity and minimal ethical and legal barriers are associated with their use. Amnion-derived stem cells have also antitumoral properties. Hepatic failure is one of the major causes of morbidity and mortality and despite the development in therapies, hepatocarcinoma rates are high worldwide. The aim of this work was to study some aspects of cell death induced by the amniotic membrane conditioned medium (AM-CM) in hepatocarcinoma cells. We have analyzed the expression of proapoptotic proteins (Caspase-3, PARP-1) by qRT-PCR and Western blot, in HepG2 and HuH-7 cells treated with AM-CM. We have also analyzed p53 expression by immunofluorescence. We found a significant increment in Caspase-3 expression and in cleaved Caspase-3 and PARP-1, after 24 and 72 h of treatment with AM-CM in hepatocarcinoma cells. We have also observed that AM-CM significant increase p53 nuclear expression. Finally, we determined that AM-CM induced DNA fragmentation after 72 h of treatment in HepG2 cells. Our results begin positioning amnion-derived stem cells as emerging candidates in anticancer therapy.