Publicado dic 16, 2019



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Mercedes OLAYA-C http://orcid.org/0000-0002-7147-425X

Myriam Beatriz Puerto https://orcid.org/0000-0002-8231-6003

Edy Marcela Corredor https://orcid.org/0000-0002-8331-3111

Carlos Humberto Pérez Moreno https://orcid.org/0000-0003-3457-2499

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Resumen

Objetivo: Introducir la prueba de desregulación del ciclo celular p16 y Ki-67 como complemento en el estudio de la evolución de las lesiones reportadas con atipia escamosa (ASC-US – LSIL – HSIL) en extendidos citológicos, dada su capacidad como marcador de progresión a cáncer por parte de lesiones precancerosas; se compara con pruebas de oro. Métodos: Estudio observacional analítico de corte transversal sobre una población a la que se le realizó citología en base líquida (CBL) y en los casos positivos, se complementó con inmunocitoquímica, cepillado endocervical, colposcopia y biopsia. Resultados: de las 51 citologías estudiadas, 35 (68,62%) fueron clasificadas como negativas y 16 (31,38%) con atipia: ASCU-US (n=8), LSIL (n=7) y HSIL (n=1). De estos 16 casos, la prueba de inmunocitoquímica fue positiva para 3 de los 7 LSIL y para el caso reportado como HSIL. Los 8 casos reportados como ASC-US fueron negativos para biomarcadores. Conclusión: el presente estudio piloto realizado a 51 pacientes, detectó cuatro de ellas con un ciclo celular desregulado, mediante inmunorreactidad a la doble tinción de P16/Ki-67; tres citologías habían mostrado LSIL y una HSIL. Los biomarcadores son un complemento útil en el tamizaje para el control de cáncer de cuello uterino, específicos para lesiones en progresión a malignidad, que se deben promover en nuestro país.

Keywords

Immunocytochemistry, Squamous intraepithelial lesions of the cervix, p16INK, papillomaInmunohistoquímica, Lesiones Intraepiteliales Escamosas de Cuello Uterino, Genes p16INK4, papilomaImunocitoquímica, Lesões Intraepiteliais Escamosas do Colo do Útero, Genes p16INK4, papilloma

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Cómo citar
OLAYA-C, M., Puerto, M. B., Corredor, E. M., & Pérez Moreno, C. H. (2019). Inmunotinción dual p16INK4a/ki-67 en citología vaginal en medio líquido. Prueba piloto de complemento diagnóstico. Universitas Medica, 61(1). https://doi.org/10.11144/Javeriana.umed61-1.dual
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