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Mercedes OLAYA-C http://orcid.org/0000-0002-7147-425X

Myriam Beatriz Puerto https://orcid.org/0000-0002-8231-6003

Edy Marcela Corredor https://orcid.org/0000-0002-8331-3111

Carlos Humberto Pérez Moreno https://orcid.org/0000-0003-3457-2499

Resumen

Objetivo: Introducir la prueba de desregulación del ciclo celular p16 y Ki-67 como complemento en el estudio de la evolución de las lesiones reportadas con atipia escamosa (ASC-US – LSIL – HSIL) en extendidos citológicos, dada su capacidad como marcador de progresión a cáncer por parte de lesiones precancerosas; se compara con pruebas de oro. Métodos: Estudio observacional analítico de corte transversal sobre una población a la que se le realizó citología en base líquida (CBL) y en los casos positivos, se complementó con inmunocitoquímica, cepillado endocervical, colposcopia y biopsia. Resultados: de las 51 citologías estudiadas, 35 (68,62%) fueron clasificadas como negativas y 16 (31,38%) con atipia: ASCU-US (n=8), LSIL (n=7) y HSIL (n=1). De estos 16 casos, la prueba de inmunocitoquímica fue positiva para 3 de los 7 LSIL y para el caso reportado como HSIL. Los 8 casos reportados como ASC-US fueron negativos para biomarcadores. Conclusión: el presente estudio piloto realizado a 51 pacientes, detectó cuatro de ellas con un ciclo celular desregulado, mediante inmunorreactidad a la doble tinción de P16/Ki-67; tres citologías habían mostrado LSIL y una HSIL. Los biomarcadores son un complemento útil en el tamizaje para el control de cáncer de cuello uterino, específicos para lesiones en progresión a malignidad, que se deben promover en nuestro país.

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Keywords

Inmunohistoquímica, Lesiones Intraepiteliales Escamosas de Cuello Uterino, Genes p16INK4, papiloma

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Cómo citar
OLAYA-C, M., Puerto, M., Corredor, E., & Pérez Moreno, C. (2019). Inmunotinción dual p16INK4a/ki-67 en citología vaginal en medio líquido. Prueba piloto de complemento diagnóstico. Universitas Médica, 61(1). https://doi.org/10.11144/Javeriana.umed61-1.dual
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