Detection of Helicobacter pylori in Adults with Dyspepsia in Ibagué, Colombia: Comparative Evaluation of Methods
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feb 16, 2024
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Angie Melissa Herrera Medina
https://orcid.org/0000-0002-1443-1579
Paula Natalia Bedoya Trujillo
https://orcid.org/0009-0005-1877-5319
Fabian Leonardo Castro Valencia
https://orcid.org/0000-0003-3338-2853
Alix Andrea Guevara Tique
https://orcid.org/0000-0003-0856-5455
John Jairo Suarez Olaya
https://orcid.org/0000-0002-8125-2351
Natalia Valderrama Arciniegas
https://orcid.org/0009-0000-6350-1361
Giovanna Parra
https://orcid.org/0009-0002-2385-620X
Mabel Elena Bohórquez
https://orcid.org/0000-0002-7679-5570
María Magdalena Echeverry de Polanco
https://orcid.org/0000-0003-4919-0821
https://orcid.org/0000-0002-1443-1579
Paula Natalia Bedoya Trujillo
https://orcid.org/0009-0005-1877-5319
Fabian Leonardo Castro Valencia
https://orcid.org/0000-0003-3338-2853
Alix Andrea Guevara Tique
https://orcid.org/0000-0003-0856-5455
John Jairo Suarez Olaya
https://orcid.org/0000-0002-8125-2351
Natalia Valderrama Arciniegas
https://orcid.org/0009-0000-6350-1361
Giovanna Parra
https://orcid.org/0009-0002-2385-620X
Mabel Elena Bohórquez
https://orcid.org/0000-0002-7679-5570
María Magdalena Echeverry de Polanco
https://orcid.org/0000-0003-4919-0821
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Resumen
Helicobacter pylori infection has been associated with various gastrointestinal disorders, including chronic gastritis, peptic ulcers, dyspepsia, and gastric cancer. This study aimed to compare the effectiveness of four invasive diagnostic methods for detecting H. pylori in 297 patients with premalignant gastric lesions and gastric cancer. In this cross-sectional study, the methods evaluated included Giemsa stain, in vitro culture, rapid urease test, and polymerase chain reaction. Patients were recruited between 2016 and 2019 in Ibagué, and a case definition criteria was used for diagnosis. Analysis of operational and epidemiological characteristics was performed to assess result agreement. Results showed H. pylori infection rates of 43% (RUT), 63% (GST), 24% (IVC), and 42% (PCR). The Kappa index demonstrated higher values for PCR (0.7704) and RUT (0.7030) compared to other methods. The RUT test displayed the highest Kappa index (0.59) when compared to PCR. Non-parametric tests indicated that PCR (0.779) and RUT (0.708) had the strongest correlation, reducing prediction error by 51.1% and 40.4%, respectively. In conclusion, the CDC criteria exhibited enhanced reliability in diagnosing H. pylori infection. Notably, PCR and RUT showed significant correlation in diagnostic accuracy for H. pylori detection.
Keywords
Helicobacter pylori, Diagnostic Accuracy, Molecular Epidemiology, Stomach Neoplasms, Precancerous conditionshelicobacter pylori, precisión diagnóstica, Epidemiología molecular, Neoplasias de estómago, Condiciones precancerosas
References
1. Li Y, Choi H, Leung K, Jiang F, Graham DY, Leung WK. Global prevalence of Helicobacter pylori infection between 1980 and 2022: a systematic review and meta-analysis. Lancet Gastroenterol Hepatol. 2023;8(6):553–64. https://doi.org/10.1016/S2468-1253(23)00070-5
2. Bravo L, Cortés A, Carrascal E, Jaramillo R, García L, Bravo P, et al. Helicobacter pylori: patología y prevalencia en biopsias gástricas en Colombia. Colombia Médica. 2003;34(3):124–31.
3. Salazar B, Gómez S, Vélez D, Ramírez V, Pérez T, Martínez A. Frecuencia de la infección por Helicobacter pylori en pacientes que requirieron endoscopia digestiva en siete unidades de tres subregiones de Antioquia. Rev Colomb Gastroenterol. 2023;38(3):290–303. https://doi.org/10.22516/25007440.983
4. Salvatori S, Marafini I, Laudisi F, Monteleone G, Stolfi C. Helicobacter pylori and gastric cancer: pathogenetic mechanisms. Int J Mol Sci. 2023;24(3):2895.
5. FitzGerald R, Smith S. An overview of Helicobacter pylori infection. Methods Mol Biol. 2021;2283:1–14. https://doi.org/10.1007/978-1-0716-1302-3_1
6. Hasna B, Houari H, Koula D, Marina S, Emilia U, Assia B. In vitro and in vivo study of combined effect of some algerian medicinal plants and probiotics against Helicobacter pylori. Microorganisms. 2023;11(5):1242. https://doi.org/10.3390/microorganisms11051242
7. Kowada A, Asaka M. Economic and health impacts of Helicobacter pylori eradication strategy for the treatment of peptic ulcer disease: a cost‐effectiveness analysis. Helicobacter. 2022;27(3):e12886.
8. Harris P, Calderón-Guerrero O, Vera-Chamorro J, Lucero Y, Vásquez M, Kazuo-Ogata S, et al. Adaptación a la realidad de Latinoamérica de la Guía Clínica NASPGHAN/ESPGHAN 2016 sobre diagnóstico, prevención y tratamiento de infección por Helicobacter pylori en pediatría. Rev Chil Pediatr. 2020;91(5):809–27.
9. Rojas-Rengifo D, Mendoza B, Jaramillo C, Rodríguez-Urrego P, Vera-Chamorro J, Álvarez J, et al. Helicobacter pylori culture as a key tool for diagnosis in Colombia. J Infect Dev Ctries. 2019;13(8):720–6.
10. Cardos A, Maghiar A, Zaha D, Pop O, Fritea L, Miere F, et al. Evolution of diagnostic methods for Helicobacter pylori infections: from traditional tests to high technology, advanced sensitivity and discrimination tools. Diagnostics. 2022;12(2):508. https://doi.org/10.3390/diagnostics12020508
11. Ansari S, Yamaoka Y. Helicobacter pylori infection, its laboratory diagnosis, and antimicrobial resistance: a perspective of clinical relevance. Clin Microbiol Rev. 2022;35:e00258–21. https://doi.org/10.1128/cmr.00258-21
12. Hussein R, Al-Ouqaili M, Majeed Y. Detection of Helicobacter pylori infection by invasive and non-invasive techniques in patients with gastrointestinal diseases from Iraq: a validation study. PLoS ONE. 2021;16(8):e0256393. https://doi.org/10.1371/journal.pone.0256393
13. Calvo A, Núñez C, González A, Chahuán J, Latorre G, et al. Validación de Sensibacter pylori test® como prueba de ureasa para el diagnóstico de infección por Helicobacter pylori en Chile. Gastroenterol Latinoam. 2016;27(3):157–61.
14. Robinson K, Atherton JC. The spectrum of Helicobacter-mediated diseases. Annu Rev Pathol. 2021;24(16):123–44.
15. López A, Delgado M, Jaramillo C, Amézquita A, Parra G, Echeverry M. Caracterización del gen de la citotoxina vacuolizante de Helicobacter pylori a partir de biopsias gástricas de pacientes residentes en Tolima, Colombia. Rev Arg Microbi. 2009;41(1):4–10.
16. Landis J, Koch G. The measurement of observer agreement for categorical data. Biometrics. 1977;33:159–74.
17. Bordin D, Voynovan I, Andreev D, Maev I. Current Helicobacter pylori diagnostics. Diagnostics. 2021;11(8):1458. https://doi.org/10.3390/diagnostics11081458
18. Godbole G, Mégraud F, Bessède E. Diagnosis of Helicobacter pylori infection. Helicobacter. 2020;25:e12735.
19. Yang H, Bing H. Diagnosis of Helicobacter pylori infection and recent advances. Diagnostics. 2021;11(8):1305. https://doi.org/10.3390/diagnostics11081305
20. Zuluaga-Arbeláez N, Sierra E, Guevara L, Pérez-Viana S. Estrategias terapéuticas para Helicobacter pylori en Colombia. CES Medicina. 2021;35(3):244–56.
21. Vazirzadeh J, Moghim S, Falahi J, Narimani T, Rafiei R, Karbasizadeh V. Comparison of four invasive methods for diagnosis of Helicobacter pylori infection: fluorescence in situ hybridization, histology, culture, rapid urease test. Mol Gen Microbiol Virol. 2020;35(2):123–8. https://doi.org/10.3103/s0891416820020111
22. Urgessa N, Geethakumari P, Kampa P, Parchuri R, Bhandari R, Alnasser A, et al. A comparison between histology and rapid urease test in the diagnosis of Helicobacter pylori in gastric biopsies: a systematic review. Cureus. 2023;15(5):e39360. https://doi.org/10.7759/cureus.39360
23. Kismat S, Tanni N, Akhtar R, Roy C, Rahman M, Molla M, et al. Diagnosis and comparison of three invasive detection methods for Helicobacter pylori infection. Microbiol Insights. 2022;15:11786361221133947. https://doi.org/10.1177/11786361221133947
24. Dechant F, Dechant R, Kandulski A, Selgrad M, Weber F, Reischl U, et al. Accuracy of different rapid urease tests in comparison with histopathology in patients with endoscopic signs of gastritis. Digestion. 2020;101(2):184–90.
25. Cagnoni M, Pagnini C, Crovaro M, Aucello A, Urgesi R, Pallotta L, et al. Evaluation of accuracy and feasibility of a new-generation ultra-rapid urease test for detection of Helicobacter pylori infection. Gastrointestinal Disorders. 2022;4(3):205–13. https://doi.org/10.3390/gidisord4030019
26. Hibaoui L, Massik A, Lebbar Z, Yahyaoui G, Mahmoud M, Bougnouch L, et al. The high sensitivity and specificity of rapid urease test in diagnosis of Helicobacter pylori infection in Moroccan children. Iran J Microbiol. 2022;14(5):669–76. https://doi.org/10.18502/ijm.v14i5.10960
27. Spiteri J, Zahra G, Schembri J, Pisani A, Borg E, Spiteri N, et al. Identification of antibiotic resistance patterns in Helicobacter pylori strains isolated from gastric biopsies using real-time PCR and genotypic analysis. Ann Gastroenterol. 2021;34(4):501–9. https://doi.org/10.20524/aog.2021.0624
28. Frías-Ordóñez J, Otero-Regino W. Aspectos prácticos en métodos diagnósticos para la infección por Helicobacter pylori: una revisión narrativa. Rev Gastroenterol Peru. 2017;37(3):246–53.
29. Otero W, Trespalacios A, Otero L, Vallejo M, Amaya M, Pardo R, et al. Guía de práctica clínica para el diagnóstico y tratamiento de la infección por Helicobacter pylori en adultos. Rev Colomb Gastroenterol. 2015;30(1):17–33.
30. Coelho L, Marinho J, Genta R, Ribeiro L, Passos M, Zaterka S, et al. IVth Brazilian consensus conference on Helicobacter pylori infection. Arq Gastroenterol. 2018;55(2):97–21. https://doi.org/10.1590/S0004-2803.201800000-20
31. Carlosama-Rosero Y, Acosta-Astaiza C, Sierra-Torres C, Bolaños-Bravo H. Genotipos de Helicobacter pylori asociados con cáncer gástrico y displasia en pacientes de Colombia. Rev Gastroenterol Méx. 2021;87(2):181–7.
2. Bravo L, Cortés A, Carrascal E, Jaramillo R, García L, Bravo P, et al. Helicobacter pylori: patología y prevalencia en biopsias gástricas en Colombia. Colombia Médica. 2003;34(3):124–31.
3. Salazar B, Gómez S, Vélez D, Ramírez V, Pérez T, Martínez A. Frecuencia de la infección por Helicobacter pylori en pacientes que requirieron endoscopia digestiva en siete unidades de tres subregiones de Antioquia. Rev Colomb Gastroenterol. 2023;38(3):290–303. https://doi.org/10.22516/25007440.983
4. Salvatori S, Marafini I, Laudisi F, Monteleone G, Stolfi C. Helicobacter pylori and gastric cancer: pathogenetic mechanisms. Int J Mol Sci. 2023;24(3):2895.
5. FitzGerald R, Smith S. An overview of Helicobacter pylori infection. Methods Mol Biol. 2021;2283:1–14. https://doi.org/10.1007/978-1-0716-1302-3_1
6. Hasna B, Houari H, Koula D, Marina S, Emilia U, Assia B. In vitro and in vivo study of combined effect of some algerian medicinal plants and probiotics against Helicobacter pylori. Microorganisms. 2023;11(5):1242. https://doi.org/10.3390/microorganisms11051242
7. Kowada A, Asaka M. Economic and health impacts of Helicobacter pylori eradication strategy for the treatment of peptic ulcer disease: a cost‐effectiveness analysis. Helicobacter. 2022;27(3):e12886.
8. Harris P, Calderón-Guerrero O, Vera-Chamorro J, Lucero Y, Vásquez M, Kazuo-Ogata S, et al. Adaptación a la realidad de Latinoamérica de la Guía Clínica NASPGHAN/ESPGHAN 2016 sobre diagnóstico, prevención y tratamiento de infección por Helicobacter pylori en pediatría. Rev Chil Pediatr. 2020;91(5):809–27.
9. Rojas-Rengifo D, Mendoza B, Jaramillo C, Rodríguez-Urrego P, Vera-Chamorro J, Álvarez J, et al. Helicobacter pylori culture as a key tool for diagnosis in Colombia. J Infect Dev Ctries. 2019;13(8):720–6.
10. Cardos A, Maghiar A, Zaha D, Pop O, Fritea L, Miere F, et al. Evolution of diagnostic methods for Helicobacter pylori infections: from traditional tests to high technology, advanced sensitivity and discrimination tools. Diagnostics. 2022;12(2):508. https://doi.org/10.3390/diagnostics12020508
11. Ansari S, Yamaoka Y. Helicobacter pylori infection, its laboratory diagnosis, and antimicrobial resistance: a perspective of clinical relevance. Clin Microbiol Rev. 2022;35:e00258–21. https://doi.org/10.1128/cmr.00258-21
12. Hussein R, Al-Ouqaili M, Majeed Y. Detection of Helicobacter pylori infection by invasive and non-invasive techniques in patients with gastrointestinal diseases from Iraq: a validation study. PLoS ONE. 2021;16(8):e0256393. https://doi.org/10.1371/journal.pone.0256393
13. Calvo A, Núñez C, González A, Chahuán J, Latorre G, et al. Validación de Sensibacter pylori test® como prueba de ureasa para el diagnóstico de infección por Helicobacter pylori en Chile. Gastroenterol Latinoam. 2016;27(3):157–61.
14. Robinson K, Atherton JC. The spectrum of Helicobacter-mediated diseases. Annu Rev Pathol. 2021;24(16):123–44.
15. López A, Delgado M, Jaramillo C, Amézquita A, Parra G, Echeverry M. Caracterización del gen de la citotoxina vacuolizante de Helicobacter pylori a partir de biopsias gástricas de pacientes residentes en Tolima, Colombia. Rev Arg Microbi. 2009;41(1):4–10.
16. Landis J, Koch G. The measurement of observer agreement for categorical data. Biometrics. 1977;33:159–74.
17. Bordin D, Voynovan I, Andreev D, Maev I. Current Helicobacter pylori diagnostics. Diagnostics. 2021;11(8):1458. https://doi.org/10.3390/diagnostics11081458
18. Godbole G, Mégraud F, Bessède E. Diagnosis of Helicobacter pylori infection. Helicobacter. 2020;25:e12735.
19. Yang H, Bing H. Diagnosis of Helicobacter pylori infection and recent advances. Diagnostics. 2021;11(8):1305. https://doi.org/10.3390/diagnostics11081305
20. Zuluaga-Arbeláez N, Sierra E, Guevara L, Pérez-Viana S. Estrategias terapéuticas para Helicobacter pylori en Colombia. CES Medicina. 2021;35(3):244–56.
21. Vazirzadeh J, Moghim S, Falahi J, Narimani T, Rafiei R, Karbasizadeh V. Comparison of four invasive methods for diagnosis of Helicobacter pylori infection: fluorescence in situ hybridization, histology, culture, rapid urease test. Mol Gen Microbiol Virol. 2020;35(2):123–8. https://doi.org/10.3103/s0891416820020111
22. Urgessa N, Geethakumari P, Kampa P, Parchuri R, Bhandari R, Alnasser A, et al. A comparison between histology and rapid urease test in the diagnosis of Helicobacter pylori in gastric biopsies: a systematic review. Cureus. 2023;15(5):e39360. https://doi.org/10.7759/cureus.39360
23. Kismat S, Tanni N, Akhtar R, Roy C, Rahman M, Molla M, et al. Diagnosis and comparison of three invasive detection methods for Helicobacter pylori infection. Microbiol Insights. 2022;15:11786361221133947. https://doi.org/10.1177/11786361221133947
24. Dechant F, Dechant R, Kandulski A, Selgrad M, Weber F, Reischl U, et al. Accuracy of different rapid urease tests in comparison with histopathology in patients with endoscopic signs of gastritis. Digestion. 2020;101(2):184–90.
25. Cagnoni M, Pagnini C, Crovaro M, Aucello A, Urgesi R, Pallotta L, et al. Evaluation of accuracy and feasibility of a new-generation ultra-rapid urease test for detection of Helicobacter pylori infection. Gastrointestinal Disorders. 2022;4(3):205–13. https://doi.org/10.3390/gidisord4030019
26. Hibaoui L, Massik A, Lebbar Z, Yahyaoui G, Mahmoud M, Bougnouch L, et al. The high sensitivity and specificity of rapid urease test in diagnosis of Helicobacter pylori infection in Moroccan children. Iran J Microbiol. 2022;14(5):669–76. https://doi.org/10.18502/ijm.v14i5.10960
27. Spiteri J, Zahra G, Schembri J, Pisani A, Borg E, Spiteri N, et al. Identification of antibiotic resistance patterns in Helicobacter pylori strains isolated from gastric biopsies using real-time PCR and genotypic analysis. Ann Gastroenterol. 2021;34(4):501–9. https://doi.org/10.20524/aog.2021.0624
28. Frías-Ordóñez J, Otero-Regino W. Aspectos prácticos en métodos diagnósticos para la infección por Helicobacter pylori: una revisión narrativa. Rev Gastroenterol Peru. 2017;37(3):246–53.
29. Otero W, Trespalacios A, Otero L, Vallejo M, Amaya M, Pardo R, et al. Guía de práctica clínica para el diagnóstico y tratamiento de la infección por Helicobacter pylori en adultos. Rev Colomb Gastroenterol. 2015;30(1):17–33.
30. Coelho L, Marinho J, Genta R, Ribeiro L, Passos M, Zaterka S, et al. IVth Brazilian consensus conference on Helicobacter pylori infection. Arq Gastroenterol. 2018;55(2):97–21. https://doi.org/10.1590/S0004-2803.201800000-20
31. Carlosama-Rosero Y, Acosta-Astaiza C, Sierra-Torres C, Bolaños-Bravo H. Genotipos de Helicobacter pylori asociados con cáncer gástrico y displasia en pacientes de Colombia. Rev Gastroenterol Méx. 2021;87(2):181–7.
Cómo citar
Herrera Medina, A. M., Bedoya Trujillo, P. N., Castro Valencia, F. L., Guevara Tique, A. A., Suarez Olaya, J. J., Valderrama Arciniegas, N., Parra, G., Bohórquez, M. E., & Echeverry de Polanco, M. M. (2024). Detection of Helicobacter pylori in Adults with Dyspepsia in Ibagué, Colombia: Comparative Evaluation of Methods. Universitas Medica, 65. https://doi.org/10.11144/Javeriana.umed65.dhpa
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