La sertralina incluida en la β-ciclodextrina no induce un perfil tolerogénico en células dendríticas derivadas de monocitos

Sindy Muñoz, Luz Stella Rodríguez

Resumen


Objetivo: Evaluar el efecto inmunomodulador de la sertralina (SRT) y la sertralina
incluida en la β-ciclodextrina (LF) en células dendríticas humanas (CD) generadas in
vitro a partir de monocitos sobre marcadores de fenotipo y la producción de citocinas.
Materiales y métodos: Se aislaron monocitos CD14+ de células mononucleares de
sangre periférica y se diferenciaron a CD; posteriormente, se pretrataron con SRT o
LF durante una hora. Finalmente, las CD se maduraron con lipopolisacárido (LPS)
durante 24 horas y se analizó el fenotipo de las CD y las concentraciones de citocinas
en los sobrenadantes de cultivo. Resultados: No se observaron cambios al comparar
el fenotipo de las CD maduradas con LPS en ausencia o presencia de la SRT. Así
mismo, no hubo variación en cuanto a la producción de citocinas. Conclusión: La
SRT incluida o no en la β-ciclodextrina no afecta el fenotipo y la secreción de las CD
tratadas con LPS.


Palabras clave


células dendríticas; sertralina; β-ciclodextrina

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Referencias


Nouws HPA, Delerue-Matos C, Barros

AA, Rodrigues JA. Electroanalytical

study of the antidepressant sertraline. J

Pharmaceut Biomed. 2005;39:290-93.

Passos JJ, De Sousa FB, Lula IS, Barreto

EA, Lopes JF, De Almeida WB, Sinisterra

RD. Multi-equilibrium system based

on sertraline and β-cyclodextrin supramolecular

complex in aqueous solution.

Int J Pharm. 2011;421(1):24-33. doi:

1016/j.ijpharm.2011.09.026

Davis ME, Brewster ME. Cyclodextrin-

based pharmaceutics: past, present

and future. Nat Rev Drug Discov.

;3:1023-35.

Furtado M, Katzman MA. Examining the

role of neuroinflammation in major depression.

Psychiat Res. 2015;229:27-36.

Lesch KP. Gene-environment interaction

and the genetics of depression. J Psychiat

Neurosci. 2004;29:174.

Felger JC, Lotrich FE. Inflammatory cytokines

in depression: neurobiological

mechanisms and therapeutic implications.

Neuroscience. 2013;246:199-229.

Dowlati Y, Herrmann N, Swardfager

W, Liu H, Sham L, Reim EK, Lanctôt

KL. A meta-analysis of cytokines

in major depression. Biol Psychiatry.

;67(5):446-57. doi: 10.1016/j.biopsych.

09.033

Branco-de-Almeida LS, Franco GC,

Castro ML, Dos Santos JG, Anbinder

AL, Cortelli SC, et al. Fluoxetine inhibits

inflammatory response and bone loss in

a rat model of ligature-induced periodontitis.

J Periodontol. 2012;83(5):664-71.

doi: 10.1902/jop.2011

Tynan RJ, Weidenhofer J, Hinwood M,

Cairns MJ, Day TA, Walker FR. A comparative

examination of the anti-inflammatory

effects of SSRI and SNRI antidepressants

on LPS stimulated microglia. Brain Behav

Immun. 2012;26:469-79. doi: 10.1016/j.

bbi.2011.12.011

Daniele S, Da Pozzo E, Zappelli E, Martini

C. Trazodone treatment protects neuronal-

like cells from inflammatory insult

by inhibiting NF-KB, p38 and JNK. Cell

Signal. 2015;27:1609-29.

Franco R, Pacheco R, Lluis C, Ahern GP,

O´Connell PJ. The emergence of neurotransmitters

as immune modulators.

Trends Immunol. 2007;28:400-7.

Mössner R, Lesch K-P. Role of serotonin

in the immune system and in neuroimmune

interactions. Brain Behav Immun.

;12:249-71.

O’Connell PJ, Wang X, Leon-Ponte M,

Griffiths C, Pingle SC, Ahern GP. A novel

form of immune signaling revealed by

transmission of the inflammatory mediator

serotonin between dendritic cells and

T cells. Blood. 2006;107(3):1010-7.

Idzko M, Panther E, Stratz C, Müller T,

Bayer H, Zissel G, et al. The serotoninergic

receptors of human dendritic cells:

identification and coupling to cytokine

release. J Immunol. 2004;172(10):6011-

Muller T, Dürk T, Blumenthal B, Grimm

M, Cicko S, Panther E, et al. 5-hydroxytryptamine

modulates migration, cytokine

and chemokine release and T-cell

priming capacity of dendritic cells in vitro

and in vivo. PLoS One. 2009;4(7):e6453.

doi: 10.1371/journal.pone.0006453

Katoh N, Soga F, Nara T, Tamagawa-Mineoka

R, Nin M, Kotani H, et al. Effect of

serotonin on the differentiation of human

monocytes into dendritic cells. Clin Exp

Immunol. 2006;146(2):354-61.

Ahern GP. 5-HT and the immune system.

Curr Opin Pharmacol. 2011;11:29-33.

Branco-de-Almeida LS, Kajiya M, Cardoso

CR, Silva MJ, Ohta K, Rosalen PL,

et al. Selective serotonin reuptake inhibitors

attenuate the antigen presentation

from dendritic cells to effector T lymphocytes.

FEMS Immunol Med Microbiol.

;62(3):283-94. doi: 10.1111/j.1574-

X.2011.00816.x

Nikolic T, Roep BO. Regulatory multitasking

of tolerogenic dendritic cellslessons

taken from vitamin d3-treated

tolerogenic dendritic cells. Front Immunol.

;4:113. doi: 10.3389/

fimmu.2013.00113

Steinman RM. Dendritic cells: understanding

immunogenicity. Eur J Immunol.

;37:S53-60.

Onishi M, Ozasa K, Kobiyama K,

Ohata K, Kitano M, Taniguchi K et al.

Hydroxypropyl-β-cyclodextrin spikes

local inflammation that induces th2 cell

and t follicular helper cell responses to

the coadministered antigen. J Immunol.

;194(6):2673-82. doi: 10.4049/jimmunol.

Lee JS, Jung ID, Lee CM, Noh KT, Park

JW, Son KH, et al. Venlafaxine inhibits

the development and differentiation of

dendritic cells through the regulation of

P-glycoprotein. Int Immunopharmacol.

;11(9):1348-57. doi: 10.1016/j.intimp.

04.019




DOI: http://dx.doi.org/10.11144/Javeriana.umed57-4.sert

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