Cultivo de células troncales de médula ósea de ratas para uso en regeneración de tejidos
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dic 13, 2022
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Antecedentes. Las células troncales se consideran un agente terapéutico prometedor en regeneración de tejidos. El uso de éstas requiere un proceso previo y riguroso de obtención y para su aplicación es esencial el uso de modelos animales. Objetivo: Obtener poblaciones de células troncales de médula ósea de ratas con la conservación en cultivo de las características de troncalidad. Métodos: Este fue un estudio experimental en el que se usaron ratas macho y hembras eutanasiadas del linaje Lewis. Se disecaron los huesos de las extremidades posteriores y, a partir de la médula ósea de estos, se obtuvieron los cultivos primarios a los cuales se les hizo la depleción de las poblaciones CD45+. Las poblaciones libres de CD45 se subcultivaron hasta el pasaje cinco y se evaluaron sus características morfológicas, inmunofenotípicas, de proliferación y la capacidad de diferenciación a tres linajes. Resultados: La evaluación morfológica de los cultivos mostró un predominio de células ahusadas y fibroblastoides que crecieron adheridos y en UFC-F. El inmunofenotipo se caracterizó por la expresión positiva de CD90, CD29 y CD146. Los cultivos inducidos a los linajes osteogénico, condrogénico y adipogénico mostraron un cambio en la morfología y positividad a las tinciones de Rojo de Alizarina, Azul Alcian y Aceite Rojo O, respectivamente. El aumento en la actividad de fosfatasa alcalina corroboró la diferenciación osteogénica en los subcultivos inducidos a este linaje. Conclusión: Se obtuvieron poblaciones de células troncales de médula ósea de ratas que conservaban las características de troncalidad y por lo tanto la posibilidad de usarlas en estudios preclínicos de regeneración de tejidos.
Keywords
biotecnología, células troncales de médula ósea, cultivo celular, diferenciación celular, ingeniería de tejidos, ratas Lewisbiotechnology, bone marrow stem cells, cell culture, cell differentiation, Lewis rats, tissue engineeringbiotecnologia, células-tronco da medula óssea, cultura de células, diferenciação celular, engenharia de tecidos, ratos Lewis
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2. Tatullo M, Codispoti B, Paduano F, Nuzzolese M, Makeeva I. Strategic Tools in Regenerative and Translational Dentistry. Int J Mol Sci. 2019 Apr 16; 20(8): 1879. https://dx.doi.org/10.3390/ijms20081879
3. McGovern JA, Griffin M, Hutmacher DW. Animal models for bone tissue engineering and modelling disease. Dis Model Mech. 2018 Apr 23; 11(4): dmm033084. https://dx.doi.org/10.1242/dmm.033084
4. Kumar R, Sharma A, Pattnaik AK, Varadwaj PK. Stem cells: An overview with respect to cardiovascular and renal disease. J Nat Sci Biol Med. 2010 Jul; 1(1): 43-52. https://dx.doi.org/10.4103/0976-9668.71674
5. Goradel NH, Hour FG, Negahdari B, Malekshahi ZV, Hashemzehi M, Masoudifar A, Mirzaei H. Stem Cell Therapy: A New Therapeutic Option for Cardiovascular Diseases. J Cell Biochem. 2018 Jan; 119(1): 95-104. https://dx.doi.org/10.1002/jcb.26169
6. Faça VM. Human mesenchymal stromal cell proteomics: contribution for identification of new markers and targets for medicine intervention. Expert Rev Proteomics. 2012 Apr; 9(2): 217-230. https://dx.doi.org/10.1586/epr.12.9
7. Yelick PC, Sharpe PT. Tooth Bioengineering and Regenerative Dentistry. J Dent Res. 2019 Oct; 98(11): 1173-1182. https://dx.doi.org /10.1177/0022034519861903
8. De Luca M, Aiuti A, Cossu G, Parmar M, Pellegrini G, Robey PG. Advances in stem cell research and therapeutic development. Nat Cell Biol. 2019 Jul; 21(7): 801-811. https://dx.doi.org/10.1038/s41556-019-0344-z
9. Liu JQ, Li QW, Tan Z. New Insights on Properties and Spatial Distributions of Skeletal Stem Cells. Stem Cells Int. 2019 Jun 3; 2019: 9026729. https://dx.doi.org/10.1155/2019/9026729
10. Bianco P, Robey PG, Saggio I, Riminucci M. "Mesenchymal" stem cells in human bone marrow (skeletal stem cells): a critical discussion of their nature, identity, and significance in incurable skeletal disease. Hum Gene Ther. 2010 Sep; 21(9): 1057-1066. https://dx.doi.org /10.1089/hum.2010.136
11. Caplan AI. Mesenchymal stem cells. J Orthop Res. 1991 Sep; 9(5): 641-650. https://dx.doi.org/10.1002/jor.1100090504
12. Samsonraj RM, Raghunath M, Nurcombe V, Hui JH, van Wijnen AJ, Cool SM. Concise Review: Multifaceted Characterization of Human Mesenchymal Stem Cells for Use in Regenerative Medicine. Stem Cells Transl Med. 2017 Dec; 6(12): 2173-2185. https://dx.doi.org /10.1002/sctm.17-0129
13. Sipp D, Robey PG, Turner L. Clear up this stem-cell mess. Nature. 2018 Sep; 561(7724): 455-457. https://dx.doi.org/10.1038/d41586-018-06756-9
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Cómo citar
Pirela Labrador, Ángel E., Tangarife Tobón, L. F., Roa Molina, N. S., Durán Correa, C., & Jaramillo Gómez, L. M. (2022). Cultivo de células troncales de médula ósea de ratas para uso en regeneración de tejidos. Universitas Odontologica, 41. https://doi.org/10.11144/Javeriana.uo41.rbms
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