Publicado dic 13, 2022



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Ángel Eduardo Pirela Labrador

Luis Felipe Tangarife Tobón

Nelly Stella Roa Molina

Camilo Durán Correa

Lorenza María Jaramillo Gómez

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Resumen

Antecedentes. Las células troncales se consideran un agente terapéutico prometedor en regeneración de tejidos. El uso de éstas requiere un proceso previo y riguroso de obtención y para su aplicación es esencial el uso de modelos animales. Objetivo: Obtener poblaciones de células troncales de médula ósea de ratas con la conservación en cultivo de las características de troncalidad. Métodos: Este fue un estudio experimental en el que se usaron ratas macho y hembras eutanasiadas del linaje Lewis. Se disecaron los huesos de las extremidades posteriores y, a partir de la médula ósea de estos, se obtuvieron los cultivos primarios a los cuales se les hizo la depleción de las poblaciones CD45+. Las poblaciones libres de CD45 se subcultivaron hasta el pasaje cinco y se evaluaron sus características morfológicas, inmunofenotípicas, de proliferación y la capacidad de diferenciación a tres linajes. Resultados: La evaluación morfológica de los cultivos mostró un predominio de células ahusadas y fibroblastoides que crecieron adheridos y en UFC-F. El inmunofenotipo se caracterizó por la expresión positiva de CD90, CD29 y CD146. Los cultivos inducidos a los linajes osteogénico, condrogénico y adipogénico mostraron un cambio en la morfología y positividad a las tinciones de Rojo de Alizarina, Azul Alcian y Aceite Rojo O, respectivamente. El aumento en la actividad de fosfatasa alcalina corroboró la diferenciación osteogénica en los subcultivos inducidos a este linaje. Conclusión: Se obtuvieron poblaciones de células troncales de médula ósea de ratas que conservaban las características de troncalidad y por lo tanto la posibilidad de usarlas en estudios preclínicos de regeneración de tejidos.

Keywords

biotecnología, células troncales de médula ósea, cultivo celular, diferenciación celular, ingeniería de tejidos, ratas Lewisbiotechnology, bone marrow stem cells, cell culture, cell differentiation, Lewis rats, tissue engineeringbiotecnologia, células-tronco da medula óssea, cultura de células, diferenciação celular, engenharia de tecidos, ratos Lewis

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Cómo citar
Pirela Labrador, Ángel E., Tangarife Tobón, L. F., Roa Molina, N. S., Durán Correa, C., & Jaramillo Gómez, L. M. (2022). Cultivo de células troncales de médula ósea de ratas para uso en regeneración de tejidos. Universitas Odontologica, 41. https://doi.org/10.11144/Javeriana.uo41.rbms
Sección
Ciencias Básicas, Biotecnología y Bioinformática