Ligia Johanna Vásquez Domínguez

Guillermo Arreola Martínez

Jaime Larriva Loyola

Adriana Rodríguez Ciódaro

Edgar Humberto Güiza Cristancho


Background: Hybrid layer formation should be proportional to cement properties to successfully retain fiberglass posts into root canals. Purpose: To measure of hybrid layer thickness in coronal and apical thirds after using one-step and two-step self-etching cements. Methods: This experimental study used 30 human premolars with root-canal treatments, which were assigned into three groups. Fiberglass posts were cemented with three types of self-etching cements following manufacturers’ instructions. Roots were cut in the coronal and apical thirds and hybrid layer thickness was quantified through scanning electron microscopy. Data ​​were analyzed with Kruskal-Wallis and Mann-Whitney tests (p < 0.05). Results: Average thickness of the hybrid layer was 0.74 μm with Multilink N™ (group 1), 0.24 μm with RelyX Ultimate™ (group 2), and 0.03 μm with RelyX U200™ (group 3) (p = 0.0092). Average thickness in coronal and apical thirds were: 1.29 μm and 0.19 μm for group 1; 0.33 μm and 0.14 μm for group 2; and 0.26 μm and 0.23 μm for group 3, differences that were statistically significant (p = 0.043). Conclusions: The thickness of the hybrid layer formed after suing two-step cements was greater than that of one-step cement, especially in the tooth coronal third.



fiber-glass post, hybrid layer, interface, one-step self-etching cement, self-etching cement, two-step self-etching cement, biomaterials, dental materials, dentistry, oral rehabilitation, prosthodontics

1. Preethi G, Kala M. Clinical evaluation of carbon fiber reinforced carbon endodontic post, glass fiber reinforced post with cast post and core: A one-year comparative clinical study. J Conserv Dent. 2008 Oct; 11(4): 162-7. doi: 10.4103/0972-0707.48841
2. Nakabayashi N, Nakamura M, Yasuda N. Hybrid layer as a dentin-bonding mechanism. J Esthet Dent. 1991 Jul-Aug; 3(4): 133-8.
3. Morgano SM, Milot P. Clinical success of cast metal posts and cores. J Prosthet Dent. 1993 Jul; 70(1): 11-6.
4. Massa F, Dias C, Blos CE, Resistance to fracture of mandibular premolars restored using post-and-core systems. Quintessence Int. 2010 Jan; 41(1): 49-57.
5. Duarte S, Sartori N, Sadan A, Phark JH. Adhesive resin cements for bonding esthetic restorations: a review. Quintessence Dent Technol. 2011 Jan; 34: 40-66.
6. Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res. 1982 May; 16(3): 265-73.
7. Ferrari M, Vichi A, Grandini S. Efficacy of different adhesive techniques on bonding to root canal walls: an SEM investigation. Dent Mater. 2001 Sep; 17(5): 422-9.
8. Sahmali S, Demirel F, Saygili G. Comparison of in vitro tensile bond strengths of luting cements to metallic and tooth-colored posts. Int J Periodontics Restorative Dent. 2004 Nov; 24(3): 256-63.
9. Sorensen JA, Engelman MJ. Effect of post adaptation on fracture resistance of endodontically treated teeth. J Prosthet Dent. 1990 Oct; 64(4): 419-24.
10. Kugel G, Ferrari M. The science of bonding: from first to sixth generation. J Am Dent Assoc. 2000 Jun; 131 Suppl: 20S-25S.
11. Pashley DH, Tay FR. Aggressiveness of contemporary self-etching adhesives. Part II: etching effects on unground enamel. Dent Mater. 2001 Sep; 17(5): 430-44.
12. Bitter K, Gläser C, Neumann K, Blunck U, Frankenberger R. Analysis of resin-dentin interface morphology and bond strength evaluation of core materials for one stage post-endodontic restorations. PLoS One. 2014 Feb; 9(2): e86294. doi: 10.1371/journal.pone.0086294
13. Sridhara KS, Mankar S, Jayshankar CM, Vinaya K. Scanning electron microscopic study of teeth restored with fiber posts and composite resin: An in vitro study. J Pharm Bioallied Sci. 2014 Jul; 6(Suppl 1): S74-S79.
14. Giachetti L, Bertini F, Scaminaci Russo D. Investigation into the nature of dentin resin tags: a scanning electron microscopic morphological analysis of demineralized bonded dentin. J Prosthet Dent. 2004 Sep; 92(3): 233-8.
15. Radovic I, Mazzitelli C, Chieffi N, Ferrari M. Evaluation of the adhesion of fiber posts cemented using different adhesive approaches. Eur J Oral Sci. 2008 Dec; 116(6): 557-63. doi: 10.1111/j.1600-0722.2008.00577.x
16. Akgungor G, Akkayan B. Influence of dentin bonding agents and polymerization modes on the bond strength between translucent fiber posts and three dentin regions within a post space. J Prosthet Dent. 2006 May; 95(5): 368-78.
17. Esclassan Noirrit E, Grégoire G, Cournot M. Morphological study of fiber-reinforced post-bonding system-root dentin interface by evaluation of two bonding systems. J Dent. 2008 Mar; 36(3): 204-13. doi: 10.1016/j.jdent.2007.12.003
18. Koshiro K, Sidhu SK, Inoue S, Ikeda T, Sano H. New concept of resin-dentin interfacial adhesion: the nanointeraction zone. J Biomed Mater Res B Appl Biomater. 2006 May; 77(2): 401-8.
19. Mannocci F, Innocenti M, Ferrari M, Watson TF. Confocal and scanning electron microscopic study of teeth restored with fiber posts, metal posts, and composite resins. J Endod. 1999 Dec; 25(12): 789-94.
20. Ozdemir E, Lin WS, Erkut S, Tuncer AH. Interfacial evaluation of endodontically treated teeth restored with selected adhesive materials and glass fiber posts: an SEM analysis. J Dent Sci. 2013 Dec; 8(4): 365-72. https://doi.org/10.1016/j.jds.2012.03.021.
21. Ferrari M, Vichi A, Grandini S, Goracci C. Efficacy of a self-curing adhesive–resin cement system on luting glass-fiber posts into root canals: an SEM investigation. Int J Prosthodont. 2001 Nov-Dec; 14(6): 543-9.
22. da Silveira Teixeira C, Santos Felippe MC, Silva-Sousa YT, de Sousa-Neto MD. Interfacial evaluation of experimentally weakened roots restored with adhesive materials and fibre posts: an SEM analysis. J Dent. 2008 Sep; 36(9): 672-82. doi: 10.1016/j.jdent.2008.04.020.
23. Giannini M, Makishi P, Ayres AP, Vermelho PM, Fronza BM, Nikaido T, Tagami J. Self-etch adhesive systems: a literature review. Braz Dent J. 2015 Jan-Feb; 26(1): 3-10. doi: 10.1590/0103-6440201302442.
24. Lui JL. Depth of composite polymerization within simulated root canals using light-transmitting posts. Oper Dent. 1994; 19: 165-8.
25. Bouillaguet S, Troesch S, Wataha JC, Microtensile bond strength between adhesive cements and root canal dentin. Dent Mater. 2003 May;19(3): 199-205.
26. Vilas Boas Motta CA, Filadelfo Silva M, Wanderley Cruz JF, Carneiro Ribeirod F, Torres León BL. The influence of 1% sodium hypoclorite in the bond strength between the radicular dentin and the fiber post using three cementing agents. Rev Odonto Cienc. 2015; 30(4): 173-78. doi: 10.15448/1980-6523.2015.3.16736
27. Tay FR, Pashley DH, Aggressiveness of contemporary self-etching systems. I: Depth of penetration beyond dentin smear layers. Dent Mater. 2001 Jul; 17(4): 296-308.
28. Koibuchi H, Yasuda N, Nakabayashi N. Bonding to dentin with a self-etching primer: the effect of smear layers. Dent Mater. 2001 Mar; 17(2): 122-6.
How to Cite
Vásquez Domínguez, L., Arreola Martínez, G., Larriva Loyola, J., Rodríguez Ciódaro, A., & Güiza Cristancho, E. (2018). Hybrid Layer Measurement after Using One-Step and Two-Step Self-Etching Cements. Universitas Odontologica, 37(78). https://doi.org/10.11144/Javeriana.uo37-78.mchr
Dental Materials and Lab
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