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Nelly Stella Roa Molina

Soledad Isabel Gómez Ramírez

Adriana Rodríguez Ciódaro

Resumen

Antecedentes: en la búsqueda de un antígeno vacunal óptimo contra la caries dental en humanos se ha utilizado el péptido PAc(365-377) de la proteína PAc de S. mutans, el cual presenta epítopes T y B para ratones. Poco se conoce acerca de la respuesta en humanos naturalmente sensibilizados. Objetivo: analizar la respuesta inmune humoral y celular frente a PAc(365-377) en relación con el estado de la caries dental. Métodos: se tomaron 30 muestras distribuidas en tres grupos: caries activa, historia de caries y libres de caries. A cada paciente se le cuantificaron las concentraciones de inmunoglobulina A y G (IgA e IgG) salivales y séricas frente al péptido por ELISA y las células productoras de las citocinas IFN-g e IL-2 (perfil TH1), IL-4, IL-5, IL-10 e IL-13 (perfil TH2) por la técnica de estimulación antígeno-específica por citometría de flujo. Resultados: se encontró una respuesta de memoria celular y de anticuerpos frente al péptido PAc(365-377) en humanos naturalmente sensibilizados. Se detectaron tres perfiles de citocinas: TH0, TH1 y TH2, con predominio del perfil TH0 por LTCD4+ y T1 por LTCD8+ en sujetos libres de caries. El 54 % de los individuos no respondió a las citocinas analizadas por LTCD4+ y el 70 % por LTCD8+. No se observaron diferencias estadísticamente significativas en la producción de anticuerpos salivales y séricos entre los grupos. Conclusiones: la respuesta inmune humoral y celular específica frente al péptido PAc(365-377) de S. mutans no muestra diferencia entre pacientes con caries e individuos resistentes a la enfermedad, en humanos naturalmente sensibilizados. Background: PAc(365-377) peptide derived of S. mutans PAc protein has been used in the search for a vaccine against dental caries in humans. This peptide has T and B epitopes in animal model (mice) but this is unknown in naturally sensitized humans. Purpose: analyze the humoral and cellular immune response to the PAc(365-377) peptide regarding dental caries status. Methods: 30 subjects were classified into three groups: active cavities, history of cavities, and caries free subjects. Blood and saliva samples were obtained from each subject. Measures included total amount of salivary and serum IgA and IgG against the PAc(365-377) peptide through ELISA and detection of functional populations of memory T cells that reacted to the PAc(365-377) peptide through frequency of IFN-g and IL-2 (TH1 profile), IL-4, IL-5, IL-10 and IL-13 (TH2 profile) with antigen-specific immune function assays through flow cytometry. Results: a memory cellular and humoral immune response against the PAc(365-377) peptide in naturally sensitized humans was observed. TH0, TH1, and TH2 profiles were detected with TH0 profile prevalence for LTCD4+ and TH1 profile prevalence for LTCD8+ in the caries free group. 54 % of subjects did not react to LTCD4+ analyzed cytokines and 70 % for LTCD8+. There were no statistically significant differences between the groups studied. Conclusions: Cellular and humoral immune response to the PAc(365-377) synthetic peptide from S. mutans did not show association with health or disease in naturally sensitized humans.

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Keywords
References
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Cómo citar
Roa Molina, N., Gómez Ramírez, S., & Rodríguez Ciódaro, A. (2015). Respuesta de células T, citocinas y anticuerpos frente al péptido (365-377) de la proteína de adhesión celular de Streptococcus mutans / Cell, Cytokine, and Antibody Response to Streptococcus mutans Cell Surface Protein Antigen Peptide (365-377). Universitas Odontologica, 33(71), 41-52. https://doi.org/10.11144/Javeriana.uo33-71.rcca
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