Técnica de diafanización para describir el desarrollo embrionario del sistema óseo. Revisión de la literatura

David Sandoval, Jelua Tellez, Guillermo Rivera, Sandra Moreno, Freddy Moreno

Resumen


Introducción: La técnica anatómica de diafanización consiste en transparentar los tejidos
blandos de organismos vertebrados (aclaramiento), para teñir los tejidos mineralizados
y poder visualizar los componentes óseos y cartilaginosos (tinción). Objetivo: Revisar
los reportes disponibles en la literatura especializada que han descrito el desarrollo
embrionario del sistema óseo de mamíferos a través de técnicas de diafanización y
tinción simple o tinción doble. Materiales y métodos: Se revisó la literatura sobre
el tema en PubMed, Google Académico y SciELO, basándose en la metodología
PRISMA, a través de la asociación del término double staining con los descriptores en
salud del Medical Subject Headings (MeSH) alizarin red, alcian blue, bone y cartilage,
y la combinación con los operadores boleanos + y &. Resultados: En esta revisión de
la literatura se incluyeron 22 artículos que describieron la técnica de diafanización
y tinción simple o tinción doble empleada en la observación, registro y análisis del 

desarrollo embrionario del sistema óseo de mamíferos.
Conclusión: La diafanización y tinción
simple o tinción doble es una técnica anatómica
de estudio —versátil y de bajo costo— del desarrollo
embrionario del sistema óseo, la cual
puede emplearse en estudios toxicológicos, para
descartar la posibilidad de anomalías de desarrollo
durante la formación del cartílago (condrogénesis)
y del hueso (osteogénesis), asociadas a la
exposición de un posible agente teratógeno.


Palabras clave


anatomía comparada; desarrollo embrionario y fetal; desarrollo óseo; hueso; cartílago

Texto completo:

PDF

Referencias


Tamayo LJ, Suárez PA, Cano AI, Cuartas

BA, Yepes SA, Mejía CA, Lenis

YY. Didactic model of the chicken

embryo development using modified

Dawson’s diaphanization and staining

technique. Rev Colomb Cienc Pecua.

;25(4):620-4.

Schultze O. Uber herstellung and conservirung

durchsichtigen embryonen zum

stadium der skeletbildung. Anat Anz.

;13:3-5.

Mall FP. On ossification centers in human

embryos less than 100 days old. Amer

Jour Anat. 1906;5:433-58.

Dawson AB. A note on the staining of the

skeleton of cleared specimens with alizarin

red S. Stain Tech. 1926;1:123-4.

Lipman HJ. Staining the skeleton of cleared

embryos with alizarin red S. Stain

Tech. 1935;10:61-3.

Cumley RW, Crow JF, Griflfen AB. Clearing

specimens for the demonstration of

bone. Stain Tech. 1939;14:7-11.

Gamble JT. A combination bleachingclearing

agent and its use in the processing

of “Spalteholtz” preparations. Stain

Tech. 1945;20:127-8.

True RM. Staining of embryonic and

small mammalian skeletal systems. Stain

Tech. 1947;22:107-8.

Staples RE, Schnell VL. Refinements in

rapid clearing technique in the KOH-alizarin

red S method for fetal bone. Stain

Technol. 1964;39:61-3.

Jensh RP, Brent RL. Rapid schedules

for KOH-clearing and alizarin red S

staining of fetal rat bone. Stain Technol.

;41:179-85.

Kawamura S, Hirohashi A, Kato T, Yasuda

M. Bone-staining technique for fetal rat specimens without skinning and

removing adipose tissue. Cong Anom.

;30:93-5.

Simons EV, Van Horn JR. A new procedure

for whole-mount alcian blue

staining of the cartilaginous skeleton of

chicken embryos, adapted to the clearing

procedure in potassium hydroxide. Acta

Morphol Neerl Scand. 1971;8:281-92.

Inouye M. Differential staining of cartilage

and bone in fetal mouse skeleton

by alcian blue and alizarin red. Congenit

Anom (Kyoto). 1976;16:171-3.

Whitaker J, Dix KM. Double staining technique

for rat fetus skeletons in teratological

studies. Lab Anim. 1979;13:309-10.

Kimmel CA, Trammel C. A rapid procedure

for routine double staining of cartilage

and bone in fetal and adult animals.

Stain Technol. 1981;56:271-3.

McLeod MJ. Differential staining of cartilage

and bone in whole mount fetuses

by Alcian blue and alizarin red S. Teratology.

;22:299-301.

Boardman JP, Mitala JJ, Carrano RA,

Iuliucci JD. Cartilagestaining technique

for the examination of unskinned fetal rat

specimens previously processed with alizarin

red S. Teratology. 1984;30:383-4.

Webb GN, Byrd RA. Simultaneous differential

staining of cartilage and bone

in rodent fetuses: an Alcian blue and

alizarin red S procedure without glacial

acetic acid. Biotechnol Histochem.

;69:181-5.

Williams TW. Alizarin red S and toluidine

blue for differentiating adult and

embryonic bone and cartilage. Slain Technology.

;16:23-5.

Burdi AR. Toluidine blue-alizarin red S

staining of cartilage and bone in wholemount

skeletons in vitro. Stain Technology.

;40:45-8.

Green MC. A rapid method for clearing

and staining specimens for the demonstration

of bone. Ohio Journal of Science.

;52(1):31-3.

Marr MC, Myers CB, George JD, Price

CJ. Comparison of single and double

staining for evaluation of skeletal development:

the effects of ethylene glycol

(EG) in CD rats. Teratology. 1988;37:476.

Erdodon D, Kadiodlu D, Peker T. Visualization

of the fetal skeletal system

by double staining with alizarin red

and alcian blue. Gazi Medical Journal.

;6:55-8.

Redfern BG, Wise LD, Spence S. An alternative

alcian blue dye variant for the

evaluation of fetal cartilage. Birth Defects

Res (Part B). 2007;80:171-6.

Setayesh MM, Esfandiari E, Rabiei AA,

Hanaei MS, Rashidi B. Comparing two

methods of plastination and glycerin

preservation to study skeletal system after

Alizarin red-Alcian blue double staining.

Adv Biomed Res. 2013;2(2):19-23.

Young AD, Phipps DE, Astroff AB.

Large-scale double-staining of rat fetal

skeletons using alizarin red S and alcian

blue. Teratology. 2000;61:273-6.

Menegola E, Broccia ML, Giavini E.

Atlas of rat fetal skeleton double stained

for bone and cartilage. Teratology.

;64:125-33.

Salaramoli J, Sadeghi F, Gilanpour H Azarnia

M, Aliesfehani T. Modified double

skeletal staining protocols with Alizarinred and Alcian blue in laboratory animals.

AMHSR. 2015;13:76-81.

Cortez-Delgado N, Pérez-Torres J, Hoyos

JM. Staining procedure of cartilage and

skeleton in adult bats and rodents. Int J

Morphol. 2009;27(4):1163-7.

Liberati A, Altman DG, Tetzlaff J, Mulrow

C, Gøtzsche PC, Ioannidis JP. The

PRISMA statement for reporting systematic

reviews and meta-analyses of studies

that evaluate health care interventions:

explanation and elaboration. PLoS Med.

;6(7):e1000100.

Randle AD. Automation of skeletal staining

in the evaluation of teratogenicity.

Laboratory Animals. 1969;3:197-205.

Tipton PW, Burt ME. A method for mechanized

staining of rat and mouse fetuses

for teratological examination. Laboratory

Animals. 1977;11(4):265-7.

Trueman D, Jackson SW, Trueman B. An

automated technique for double staining

rat and rabbit fetal skeletal specimens to

differentiate bone and cartilage. Biotech

Histochem. 1999;74(2):98-104.

Nagy A, Gertsenstein M, Vintersten K,

Behringer R. Alizarin red staining of

post-natal bone in mouse. Cold Spring

Harb Protoc. 2009;3.

Ovchinnikov D. Alcian blue/alizarin red

staining of cartilage and bone in mouse.

Cold Spring Harbor Protoc. 2009;3.

doi:10.1101/pdb.prot5170

Rasweilier JJ, Cretekos CJ, Behringer

RR. Alcian blue/alizarin red staining of

cartilage and bone of short-tailed fruit

bat (Carollia perspicillata). Cold Spring

Harb Protoc; 2009;3. doi: 10.1101/pdb.

prot5165

Wise LD, Winkelmann CT. Evaluation

of hydroxyurea-induced fetal skeletal

changes in Dutch belted rabbits by micro-

computed tomography and alizarin

red staining. Birth Defects Res (Part B).

;86:220-6.

Wise LD, Winkelmann CT. Micro-computed

tomography and alizarin red evaluations

of boric acid-induced fetal skeletal

changes in Sprague-Dawley rats. Birth

Defects Res (Part B). 2009;86(3):214-9.

Schneider S. Skeletal examination by

double staining for ossified bone and

cartilaginous tissue. Methods Mol Biol.

;947:215-21.

Riqueur D, Lyons KM. Whole-mount

skeletal staining. Methods Mol Biol.

;1130:113-21.

Revnaud L, Jocteur-Monrozier A. Skeletal

examination by alizarin staining.

Methods Mol Biol. 2013;947:201-13.

Trueman D, Stewart J. An automated

technique for double staining mouse fetal

neonatal skeletal specimens to differentiate

bone and cartilage. Biotech Histochem.

;89(4):315-9.

Ross MH, Pawlina W. Histología: Texto

y atlas color con biología celular y molecular.

a ed. Buenos Aires: Editorial

Médica Panamericana; 2007.

Geneser F. Histología. 3a ed. Madrid:

Editorial Médica Panamericana; 2000.

Gartner LP, Hiatt JL. Texto atlas de histología.

a ed. México: McGraw-Hill;

Cui D. Histología con correlaciones funcionales

y clínicas. Barcelona: Wolters

Kluwer; 2011.

Lowe JS, Anderson PG. Stevens y Lowe

histología humana. 4a ed. México: Elsevier-

Mosby; 2015.

Meloan SN, Puchtler H, Valentine LS. Alkaline

and acid alizarin red S stains for alkali-

soluble and alkali-insoluble calcium

deposits. Arch Pathol. 1972;93(3):19-27.

Dingerkus G, Uhler LD. Enzyme clearing

of alcian blue stained whole small

vertebrates for demonstration of cartilage.

Stain Technol. 1977;52(4):229-32.

Yamazaki Y, Yuguchi M, Kubota S, Isokawa.

Whole-mount bone and cartilage

staining of chick embryos with minimal

decalcification. Biotech Histochem.

;86(5):351-8.




DOI: http://dx.doi.org/10.11144/Javeriana.umed57-4.tddd

Métricas de artículo

Cargando métricas ...

Metrics powered by PLOS ALM




Copyright (c) 2017 David Sandoval, Jelua Tellez, Guillermo Rivera, Sandra Moreno, Freddy Moreno

Licencia de Creative Commons
Esta obra está registrada bajo una licencia de Creative Commons Reconocimiento 4.0 Internacional.
Creado a partir de http://revistas.javeriana.edu.co/.