Análise Estrutural da Fibrina Rica em Plaquetas e suas Aplicações na Odontologia Regenerativa
https://orcid.org/0000-0002-8921-1327
Juan Pablo Hinojosa Sarria
https://orcid.org/0000-0001-7410-9007
Ana Isabel Restrepo Dorado
https://orcid.org/0000-0001-7900-9638
Ana Luisa Muñoz Ramírez
https://orcid.org/0000-0002-7566-2979
Néstor Fabián Velarde Trochez
https://orcid.org/0000-0002-5358-7912
Francy Ester Bastidas Daza
https://orcid.org/0000-0002-0725-683X
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Antecedentes: A fibrina rica em plaquetas (PRF) é um concentrado de plaquetas que é utilizado com maior frequência em medicina e odontologia. Os resultados clínicos são variáveis porque existem diferentes protocolos, equipamentos de centrifugação e técnicas de colocação. A ignorância dos elementos estruturais pode afetar o resultado clínico. Objetivo: Descrever as características estruturais da Fibrina Rica em Plaquetas nas diferentes zonas da membrana. Métodos: Estudo experimental in vitro com 15 amostras de sangue de cinco voluntários adultos saudáveis atendidos na clínica odontológica da Universidade Antonio Nariño, Popayán. O hemograma basal foi feito, o sangue foi coletado e centrifugado (10 minutos x 3000 rpm). As amostras foram analisadas histologicamente e com microscopia eletrônica de varredura (MEV). A estrutura da fibrina, plaquetas e leucócitos foi descrita. Resultados: A contagem média de plaquetas no sangue total foi de 251 ± 31,74 x103 x mm3 e na PRF foi de 832 ± 123,43 x103 x mm3. Macroscopicamente, três áreas do PRF foram identificadas: uma superior com poucas plaquetas (FPP), uma camada leucoplaquetária (BC) e a zona da rede corpuscular (RBC). Na análise da microscopia óptica, observa-se que na área da CB há uma maior concentração de plaquetas. A análise SEM prova que a estrutura da rede de fibrina e o conteúdo celular são diferenciais em cada zona. Conclusão: A partir do conhecimento estrutural do PRF, aplicações que podem melhorar o desempenho do biomaterial e, portanto, os resultados clínicos podem ser propostos.
dentistry, tissue regeneration, regenerative dentistry, platelet concentrates, platelet-rich fibrin, scanning electron microscopyregeneración tisular, odontología, odontología regenerativa, concentrados plaquetarios, fibrina rica en plaquetas, microscopía electrónica de barridoodontologia, regeneração tecidual, odontologia regenerativa, concentrados de plaquetas, fibrina rica em plaquetas, microscopia eletrônica de varredura
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