The Effect of Capsaicine in Human Pulp Fibroblasts, in the Production of PGE2 and Proinflammatory Cytokines...

María Alexandra Bedoya Mejía, Luz Stella Rodríguez Camacho, Lorenza María Jaramillo Gómez, Gloria Cristina Moreno Abello


ABSTRACT. Background: due of capsaicin effect on the control of various inflammatory mediators, it has been proposed to modulate inflammatory processes caused by physical assaults to pulp tissue. Purpose: to evaluate the effect of capsaicin diluted in 0.05 % ethanol and its vehicle on PGEproduction, IL-8, IL-6, IL-1β, and IL-12p70 in Human Fibroblasts Pulp (FPH). Methods: concentrations of PGE2, IL-8, IL-6, IL-1β, and IL-12p70 were analyzed by ELISA or flow cytometry in supernatants of FPH at 6 and 8 hours, after being stimulated with capsaicin 5*10-5 M and 2,5*10-5 M and ethanol 0.05% vehicle of capsaicin and compared with unstimulated cells. Results: ethanol 0.05% as a diluent capsaicin, is responsible for the effect on the decrease in the expression of PGE2, IL-8 and IL-6 at the times and concentrations evaluated; and IL-12p-70 was the only cytokine that increased significantly in the presence of capsaicin 2,5*10-5 M at 8h, it is statistically greater than ethanol 0.05%. Capsaicin and ethanol had not significant effect on IL-1β. Conclusion: the effect of capsaicin on FPH, is associated with ethanol 0.05% used as diluent and varies depending on time and the mediator analyzed.


capsaicina; endodoncia; enfermedades pulpares; etanol; fibroblastos; interleuquinas; prostaglandina E; pulpa dental

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