Antígeno proteico de superficie celular (PAc) de Streptococcus mutans
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Resumen
Streptococcus mutans (S. mutans) es el principal microorganismo asociado a caries dental en humanos. Presenta en su superficie una proteína, PAc, que es responsable de la adhesión inicial de éste a la película adquirida; su peso molecular ha sido reportado en la mayoría de los estudios de 190 KDa; está constituida por 1561 aminoácidos, codificados por un gen de 4695 pb, denominado pac. Esta proteína ha sido considerada como posible antígeno vacunal contra la caries porque en su interior se encuentran dos regiones repetitivas de gran interés; una es la región repetitiva A; rica en alanina, está localizada en el extremo terminal amino, en el cual se ha identificado un segmento importante cuya secuencia comprende los aminoácidos 365 a 377, identificado como epítope celular T y B, además de ser epítope de adhesión. La otra región repetitiva es conocida como P, por ser rica en prolina; se encuentra localizada hacia el centro de la molécula y dentro de ésta se han identificado varios epítopes celulares T, B y de adhesión, destacándose principalmente el segmento comprendido entre los aminoácidos 1095-1114, el cual contiene los tres epítopes de interés.
Keywords
Streptococcus mutans, vacuna, caries dental, PAc, epítope B, epítope de adhesión, péptidos, antígenos de superficie, microbiología oral, cariologíaStreptococcus mutans, vaccine, dental caries, PAc, B epitope, T epitope, adhesion epitope, peptide, surface protein antigen, oral microbiology, cariology
References
1. Russell MW, Lehner T. Characterization of antigen extracted from cells and culture fluids of Streptococcus mutans serotype c. Arch Oral Biol 1978; 23(1): 7-15
2. Russell RB. Wall-associated protein antigens of Streptococcus mutans. J Gen Microbiol 1979; 114: 109-15
3. Hughes M, Machardy SM, Sheppard AJ, Woods NC. Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue. Infect Immun 1980; 27: 576-88
4. Forester H, Hunter N, Knox KW. Characteristics of high molecular weight extracelular protein of Streptococcus mutans. J Gen Microbiol 1983 Sep; 129(Pt 9): 2779-88
5. Ackermans F, Klein JP, Ogier J, Bazin H, Cormont F, Frank RM. Purification and characterization of a saliva interacting cellwall protein from Streptococcus mutans serotype f by using monoclonal-antibody immunoaffinity chromatography. Biochem J 1985 May 15; 228(1): 211-7
6. Okahashi N, Sasakawa C, Yoshikawa M, Hanada S, Koga T. Molecular characterization of a protein antigen gene from serotype c Streptococcus mutans implicated in dental caries. Mol Microbiol 1989; 3: 673-8
7. Demuth DR, Golub EE, Malamud D. Streptococcalhost interactions: structural and functional analysis of a Streptococcus sanguis receptor for a human salivary glycoprotein. J Biol Chem 1990 May 5; 265(13): 7120-26
8. Russell MW. Immunization against dental caries. Curr Opin Dent 1992 Sep; 2: 72-80
9. Russell MW, Bergmeier LA, Zanders ED, Lehner T. Protein antigens of Streptococcus mutans: Purification and properties of a double antigen and its protease-resistant component. Infect Immun 1980 May; 28(2): 486-93
10. Senpuku H, Miyauchi T, Hanada N, Nisizawa T. An antigenic peptide inducing cross-reacting antibodies inhibiting the interaction of Streptococcus mutans PAc with human salivar components. Infect Immun 1995 Dec; 63(12): 4695-703
11. Murakami Y, Nakano Y, Yamashita Y, Koga T. Identification of a frameshift mutation resulting in premature termination an loss of cell wall anchoring of the PAc antigen of Streptococcus mutans GS-5. Infect Immun 1997 Feb; 65(2): 794-7
12. Homonylo-McGavin MK, Lee SF. Role of the C terminus in antigen P1 surface localization in Streptococcus mutans and two related cocci. J Bacteriol 1996; 173(3): 801-7
13. LaPolla RJ, Haron JA, Kelly CG, Taylor WR, Bohart CH, Hendriks M et al. Sequence and structural analysis of surface protein antigen I/II (SpaA) of Streptococcus sobrinus. Infect Immun 1991; 59(8): 2677-85
14. Koga T, Okahashi I, Kanamoto T, Asakawa H, Iwaka I. Surface hydrophobicity, adherence, and aggregation of cell surface protein antigenmutants of Streptococcus mutans serotype c. Infect Immun 1990; 58(2): 289-96
15. Ma JKC, Kelly CG, Munro G, Whiley RA, Lehner T. Conservation of the gene encoding Streptococcal antigen I/II in oral Streptococci. Infect Immun 1991; 59(8): 2686-94
16. Koga T, Okahashi N, Takahashi I. Molecular characterization of a surface protein antigen from Streptococcus mutans and application of the antigen to a caries vaccine. Predictable advances in oral health research. Japan: Tokyo Dental College, 1991; 38-54
17. Murakami Y, Yamashita Y, Nakano Y, Ito H-O et al. Role of charged tail in localization of a surface Protein antigen of Streptococcus mutans. Infect Immun 1997; 65(4): 1531-5
18. Bowen WH, Schilling K, Giertsen E, Pearson S, Lee F, Bleiweis A et al. Role of a surface– associated protein in adherence and dental caries. Infect Immun 1991; 59(12): 4606-9
19. Russell MW, Mansson-Rahemtulla B. Interaction between surface protein antigens of Streptococcus mutans and human salivary components. Oral Microbiol Immunol 1989; 4: 106-11
20. Hajishengallis G, Koga T, Russell MW. Affinity and specificity of the interactions between Streptococcus mutans antigen I/II and salivary components. J Dent Res 1994 Sep; 73(9): 1493- 502
21. Crowley PJ, Brady LJ, Piacentini DA, Bleiweis AS. Identification of a salivary agglutininbinding domain within cell surface adhesin P1 of Streptococcus mutans. Infect Immun 1993; 61(4): 1547-52
22. Nakai M, Okahashi N, Ohta-H, Koga T. Saliva binding region of Streptococcus mutans surface protein antigen. Infect Immun 1993; 61(10): 4344-49
23. Love MR, McMillan MD, Jenkinnson HF. Invasion of dentinal tubules by oral streptococci is associated with collagen recognition mediated by the antigen I/II family of polypeptides. Infect Immun 1997; 65(12): 5157-64
24. Senpuku H, Izima T, Yamaguchi Y, Nagata S, Ueno Y, Saito M et al. Immunogenicity of peptides coupled with multiple T cell epitopes of a surface protein antigen of Streptococcus mutans. Immunology 1996 Jun; 88(2): 275-83
25. Wu HY, Russell MW. Immunological crossreactivity between Streptococcus mutans and human heart tissue examined by crossimmunization experiments. Infect Immun 1990 Nov; 58(11): 3545-52
26. Lehner T, Walker P, Bergmeier LA, Haron JA. Immunogenicity of synthetic peptides derived from the sequences of a Streptococcus mutans cell surface antigen in nonhuman primates. J Immunol 1989 Oct 15; 143(8): 2699-705
27. Takahashi I, Okahashi N, Matsushita K, Tokuda M, Kanamoto T, Munekata E et al. Immunogenicity and protective effect against oral colonization by Streptococcus mutans of synthetic peptides of a streptococcal surface protein antigen. J Immunol 1991 Jan 1; 146(1): 332-6
28. Takahashi I, Matsushita K, Nisizawa T, Okahashi N, Russell MW et al. Genetic control of immune responses in mice to synthetic peptides of a Streptococcus mutans surface protein antigen. Infect Immun 1992 Feb; 60(2): 623-29
29. Okahashi N, Takahashi I, Nakai M, Senpuku H, Nisizawa T, Koga T. Identification of antigenic epitopes in an alanine-rich repeating region of a surface protein antigen of Streptococcus mutans. Infect Immun Apr 1993; 61(4): 1301-6
30. Matsushita K, Nisizawa T, Nagaoka S, Kawagoe M, Koga T. Identification of antigenic epitopes in a surface protein antigen of Streptococcus mutans in humans. Infect Immun1994 Sep; 62(9): 4034-42
31. Senpuku H, Yanagi K, Nisizawa T. Identification of Streptococcus mutans PAc peptide motif binding with human MHC class II molecules (DRB1*0802, *1101, *1401, *1405). Immunology 1998 Nov; 95(3): 322-30
32. Kato H, Takeuchi H, Oishi Y, Senpuku H, Shimura N, Hanada N et al. The Immunogenicity of various peptide antigens inducing cross-reacting antibodies to cell surface protein antigen of Streptococcus mutans. Oral Microbiol Immunol 1999 Aug; 14(4): 213-9
33. Oishi Y, Onozuka A, Kato H, Shimura N, Imai S, Nisizawa T. The effect of amino acid spacers on the antigenicity of dimeric peptide-inducing crossreacting antibodies to a cell surface protein of Streptococcus mutans. Oral Microbiol Immunol 2001 Feb; 16(1): 40-4
34. Munro GH, Evans P, Todryk S, Buckett P, Kelly CG, Lehner T. A protein fragment of Streptococcal cell surface antigen I/II which prevents adhesion or Streptococcus mutans. Infect Immun 1993 Nov; 61(11): 4590-8
35. Kelly CG, Todryk S, Kendal HL, Munro GH, Lehner T. T-cell, adhesion, and B-cell epitopes of the cell surface Streptococcus mutans protein antigen I/II. Infect Immun 1995 Sep; 63(9): 3649-58
36. Todryk SM, Kelly CG, Munro GH, Lehner T. Induction of immune responses to functional determinants of a cell surface streptococcal antigen. Immunology 1996 Jan; 87(1): 55-63
37. Kelly CG, Younson JS, Hikmat BY, Todryk SM,Czisch M, Haris P et al. Synthetic peptide adhesion epitope as a novel antimicrobial agent. Nat Biotech 1999 Jan; 17(1): 42-7
38. Brady LJ, Cvitkovitch DG, Geric CM, Addison MN. Joyce C, Crowley PJ et al. Deletion of the central proline-rich repeat domain results in altered antigenicity and lack of surface expression of the Streptococcus mutans P1 adhesin molecule. Infect Immun 1998 Sep: 66(9): 4274-82
2. Russell RB. Wall-associated protein antigens of Streptococcus mutans. J Gen Microbiol 1979; 114: 109-15
3. Hughes M, Machardy SM, Sheppard AJ, Woods NC. Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue. Infect Immun 1980; 27: 576-88
4. Forester H, Hunter N, Knox KW. Characteristics of high molecular weight extracelular protein of Streptococcus mutans. J Gen Microbiol 1983 Sep; 129(Pt 9): 2779-88
5. Ackermans F, Klein JP, Ogier J, Bazin H, Cormont F, Frank RM. Purification and characterization of a saliva interacting cellwall protein from Streptococcus mutans serotype f by using monoclonal-antibody immunoaffinity chromatography. Biochem J 1985 May 15; 228(1): 211-7
6. Okahashi N, Sasakawa C, Yoshikawa M, Hanada S, Koga T. Molecular characterization of a protein antigen gene from serotype c Streptococcus mutans implicated in dental caries. Mol Microbiol 1989; 3: 673-8
7. Demuth DR, Golub EE, Malamud D. Streptococcalhost interactions: structural and functional analysis of a Streptococcus sanguis receptor for a human salivary glycoprotein. J Biol Chem 1990 May 5; 265(13): 7120-26
8. Russell MW. Immunization against dental caries. Curr Opin Dent 1992 Sep; 2: 72-80
9. Russell MW, Bergmeier LA, Zanders ED, Lehner T. Protein antigens of Streptococcus mutans: Purification and properties of a double antigen and its protease-resistant component. Infect Immun 1980 May; 28(2): 486-93
10. Senpuku H, Miyauchi T, Hanada N, Nisizawa T. An antigenic peptide inducing cross-reacting antibodies inhibiting the interaction of Streptococcus mutans PAc with human salivar components. Infect Immun 1995 Dec; 63(12): 4695-703
11. Murakami Y, Nakano Y, Yamashita Y, Koga T. Identification of a frameshift mutation resulting in premature termination an loss of cell wall anchoring of the PAc antigen of Streptococcus mutans GS-5. Infect Immun 1997 Feb; 65(2): 794-7
12. Homonylo-McGavin MK, Lee SF. Role of the C terminus in antigen P1 surface localization in Streptococcus mutans and two related cocci. J Bacteriol 1996; 173(3): 801-7
13. LaPolla RJ, Haron JA, Kelly CG, Taylor WR, Bohart CH, Hendriks M et al. Sequence and structural analysis of surface protein antigen I/II (SpaA) of Streptococcus sobrinus. Infect Immun 1991; 59(8): 2677-85
14. Koga T, Okahashi I, Kanamoto T, Asakawa H, Iwaka I. Surface hydrophobicity, adherence, and aggregation of cell surface protein antigenmutants of Streptococcus mutans serotype c. Infect Immun 1990; 58(2): 289-96
15. Ma JKC, Kelly CG, Munro G, Whiley RA, Lehner T. Conservation of the gene encoding Streptococcal antigen I/II in oral Streptococci. Infect Immun 1991; 59(8): 2686-94
16. Koga T, Okahashi N, Takahashi I. Molecular characterization of a surface protein antigen from Streptococcus mutans and application of the antigen to a caries vaccine. Predictable advances in oral health research. Japan: Tokyo Dental College, 1991; 38-54
17. Murakami Y, Yamashita Y, Nakano Y, Ito H-O et al. Role of charged tail in localization of a surface Protein antigen of Streptococcus mutans. Infect Immun 1997; 65(4): 1531-5
18. Bowen WH, Schilling K, Giertsen E, Pearson S, Lee F, Bleiweis A et al. Role of a surface– associated protein in adherence and dental caries. Infect Immun 1991; 59(12): 4606-9
19. Russell MW, Mansson-Rahemtulla B. Interaction between surface protein antigens of Streptococcus mutans and human salivary components. Oral Microbiol Immunol 1989; 4: 106-11
20. Hajishengallis G, Koga T, Russell MW. Affinity and specificity of the interactions between Streptococcus mutans antigen I/II and salivary components. J Dent Res 1994 Sep; 73(9): 1493- 502
21. Crowley PJ, Brady LJ, Piacentini DA, Bleiweis AS. Identification of a salivary agglutininbinding domain within cell surface adhesin P1 of Streptococcus mutans. Infect Immun 1993; 61(4): 1547-52
22. Nakai M, Okahashi N, Ohta-H, Koga T. Saliva binding region of Streptococcus mutans surface protein antigen. Infect Immun 1993; 61(10): 4344-49
23. Love MR, McMillan MD, Jenkinnson HF. Invasion of dentinal tubules by oral streptococci is associated with collagen recognition mediated by the antigen I/II family of polypeptides. Infect Immun 1997; 65(12): 5157-64
24. Senpuku H, Izima T, Yamaguchi Y, Nagata S, Ueno Y, Saito M et al. Immunogenicity of peptides coupled with multiple T cell epitopes of a surface protein antigen of Streptococcus mutans. Immunology 1996 Jun; 88(2): 275-83
25. Wu HY, Russell MW. Immunological crossreactivity between Streptococcus mutans and human heart tissue examined by crossimmunization experiments. Infect Immun 1990 Nov; 58(11): 3545-52
26. Lehner T, Walker P, Bergmeier LA, Haron JA. Immunogenicity of synthetic peptides derived from the sequences of a Streptococcus mutans cell surface antigen in nonhuman primates. J Immunol 1989 Oct 15; 143(8): 2699-705
27. Takahashi I, Okahashi N, Matsushita K, Tokuda M, Kanamoto T, Munekata E et al. Immunogenicity and protective effect against oral colonization by Streptococcus mutans of synthetic peptides of a streptococcal surface protein antigen. J Immunol 1991 Jan 1; 146(1): 332-6
28. Takahashi I, Matsushita K, Nisizawa T, Okahashi N, Russell MW et al. Genetic control of immune responses in mice to synthetic peptides of a Streptococcus mutans surface protein antigen. Infect Immun 1992 Feb; 60(2): 623-29
29. Okahashi N, Takahashi I, Nakai M, Senpuku H, Nisizawa T, Koga T. Identification of antigenic epitopes in an alanine-rich repeating region of a surface protein antigen of Streptococcus mutans. Infect Immun Apr 1993; 61(4): 1301-6
30. Matsushita K, Nisizawa T, Nagaoka S, Kawagoe M, Koga T. Identification of antigenic epitopes in a surface protein antigen of Streptococcus mutans in humans. Infect Immun1994 Sep; 62(9): 4034-42
31. Senpuku H, Yanagi K, Nisizawa T. Identification of Streptococcus mutans PAc peptide motif binding with human MHC class II molecules (DRB1*0802, *1101, *1401, *1405). Immunology 1998 Nov; 95(3): 322-30
32. Kato H, Takeuchi H, Oishi Y, Senpuku H, Shimura N, Hanada N et al. The Immunogenicity of various peptide antigens inducing cross-reacting antibodies to cell surface protein antigen of Streptococcus mutans. Oral Microbiol Immunol 1999 Aug; 14(4): 213-9
33. Oishi Y, Onozuka A, Kato H, Shimura N, Imai S, Nisizawa T. The effect of amino acid spacers on the antigenicity of dimeric peptide-inducing crossreacting antibodies to a cell surface protein of Streptococcus mutans. Oral Microbiol Immunol 2001 Feb; 16(1): 40-4
34. Munro GH, Evans P, Todryk S, Buckett P, Kelly CG, Lehner T. A protein fragment of Streptococcal cell surface antigen I/II which prevents adhesion or Streptococcus mutans. Infect Immun 1993 Nov; 61(11): 4590-8
35. Kelly CG, Todryk S, Kendal HL, Munro GH, Lehner T. T-cell, adhesion, and B-cell epitopes of the cell surface Streptococcus mutans protein antigen I/II. Infect Immun 1995 Sep; 63(9): 3649-58
36. Todryk SM, Kelly CG, Munro GH, Lehner T. Induction of immune responses to functional determinants of a cell surface streptococcal antigen. Immunology 1996 Jan; 87(1): 55-63
37. Kelly CG, Younson JS, Hikmat BY, Todryk SM,Czisch M, Haris P et al. Synthetic peptide adhesion epitope as a novel antimicrobial agent. Nat Biotech 1999 Jan; 17(1): 42-7
38. Brady LJ, Cvitkovitch DG, Geric CM, Addison MN. Joyce C, Crowley PJ et al. Deletion of the central proline-rich repeat domain results in altered antigenicity and lack of surface expression of the Streptococcus mutans P1 adhesin molecule. Infect Immun 1998 Sep: 66(9): 4274-82
Cómo citar
Jaramillo Gómez, L. M., & Gómez Ramírez, S. I. (2021). Antígeno proteico de superficie celular (PAc) de Streptococcus mutans. Universitas Odontologica, 24(54-55), 125–131. Recuperado a partir de https://revistas.javeriana.edu.co/index.php/revUnivOdontologica/article/view/13579
Sección
Ciencias Básicas, Biotecnología y Bioinformática
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