María Alexandra Bedoya Mejía

Luz Stella Rodríguez Camacho

Lorenza María Jaramillo Gómez

Gloria Cristina Moreno Abello


ABSTRACT. Background: due of capsaicin effect on the control of various inflammatory mediators, it has been proposed to modulate inflammatory processes caused by physical assaults to pulp tissue. Purpose: to evaluate the effect of capsaicin diluted in 0.05 % ethanol and its vehicle on PGE2 production, IL-8, IL-6, IL-1β, and IL-12p70 in Human Fibroblasts Pulp (FPH). Methods: concentrations of PGE2, IL-8, IL-6, IL-1β, and IL-12p70 were analyzed by ELISA or flow cytometry in supernatants of FPH at 6 and 8 hours, after being stimulated with capsaicin 5*10-5 M and 2,5*10-5 M and ethanol 0.05% vehicle of capsaicin and compared with unstimulated cells. Results: ethanol 0.05% as a diluent capsaicin, is responsible for the effect on the decrease in the expression of PGE2, IL-8 and IL-6 at the times and concentrations evaluated; and IL-12p-70 was the only cytokine that increased significantly in the presence of capsaicin 2,5*10-5 M at 8h, it is statistically greater than ethanol 0.05%. Capsaicin and ethanol had not significant effect on IL-1β. Conclusion: the effect of capsaicin on FPH, is associated with ethanol 0.05% used as diluent and varies depending on time and the mediator analyzed.


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How to Cite
Bedoya Mejía, M., Rodríguez Camacho, L., Jaramillo Gómez, L., & Moreno Abello, G. (2016). The Effect of Capsaicine in Human Pulp Fibroblasts, in the Production of PGE2 and Proinflammatory Cytokines. Universitas Odontologica, 35(74), 75-83. https://doi.org/10.11144/Javeriana.uo35-74.ecfp
Basic Sciences, Biotechnology and Bioinformatics