Evaluation of Platelet-Rich Plasma Effect at different times and concentrations on periodontal ligament fibroblast and osteoblast viability

Adriana Paola Acosta Gómez, Sandra Janeth Gutiérrez Prieto, María Alexandra Bedoya Mejía, Dabeiba Adriana García Robayo, Ximena Edilia Moreno Serrano

Abstract


ABSTRACT. Background: Growth factors used in health treatments can be obtained from a first-generation source called platelet-rich plasma. The variety of protocols to prepare PRP produces variable results regarding PRP activation time and its effects on cell proliferation and viability. Purpose: To evaluate proliferation and cell viability of periodontal ligament fibroblasts and osteoblasts stimulated with PRP in several concentrations and times after PRP activation. Methods: An in vitro study was carried out using periodontal ligament fibroblast and osteoblast cell cultures. PRP from venous blood of a healthy adult was prepared through centrifugation and activated with 10 % CaCl2. The effect on cell proliferation after application of 1 %, 3 %, and 5% PRP and platelet-poor plasma was evaluated at 0, 12, 24, 48, and 72 hours after activation through MTS. The control group consisted of culture that did not receive any treatment. Data were analyzed using Chi square, Fisher, and McNemar tests. Results: The cell viability assay showed statistically significant differences between the experimental and the control groups. Cell viability increased in cells treated with 5 % PRP 24 hours after activation (p=0.05). Conclusions: Fibroblast and osteoblast cell lines tended to be more viable 24 hours after activation with 5% PRP.

Keywords


molecular biology; periodontics; cell cultures; cell proliferation; cell viability; in vitro; osteoblast; platelet-rich plasma; periodontal ligament fibroblast

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DOI: http://dx.doi.org/10.11144/Javeriana.uo36-76.eepr

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Copyright (c) 2017 Adriana Paola Acosta Gómez, Sandra Janeth Gutiérrez Prieto, María Alexandra Bedoya Mejía, Dabeiba Adriana García Robayo, Ximena Edilia Moreno Serrano

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