In Vitro Evaluation of Enterococcus faecalis Microleakage Using Five Obturation Techniques

Silvia Elena Aragón Matamoros, Tomás Alberto Guindos Moya, Yanina Meza González, Diana Morales Herrera, María Alejandra Perera Díaz, Adriana Rodríguez Ciódaro, Hugo Diez Ortega, Catalina Méndez De la Espriella

Abstract


ABSTRACT. Background: The root canal filling technique named Hybrid-Mixed Condensation, combines the advantages of cold lateral and warm vertical condensation. The ability of avoiding microbial microleakage has not been proven. Purpose: To evaluate the differences of microbial microleakage using Enterococcus faecalis, in canals obturated with five different techniques: lateral, warm vertical, WaveOne® single cone, Guttacore®, and Hybrid-mixed condensation. Methods: 50 single-rooted human premolars extracted for orthodontic reasons were biomechanical prepared with primary file of WaveOne® system. Teeth were divided into 5 groups using different obturation techniques: single cone with WaveOne® Primary, lateral condensation using 2 % gutta-percha cones, Guttacore® 30, warm vertical condensation using down packing in a WaveOne® Primary cone and backfill with alpha gutapercha of Beefill®, and the hybrid mixed condensation modifying the lateral condensation with heat and a backfill using Beefill®. Enterococcus faecalis was inoculated in the coronal third and apices were immersed in brain heart infusion broth with phenol red incubated at 37 °C for 12 weeks. Microfiltration was determined with color change and turbidity of the medium. Specimens were observed by scanning electron microscopy. Results: Only 11 teeth (22 %) were positive for leakage. 46 % with single cone, 30 % with Guttacore®, 20 % with lateral condensation, 10 % with warm vertical condensation and no microleakage was found for Hybrid-Mixed Technique over the period of 12 weeks of study. Conclusion: Hybrid Mixed Technique showed to be the most efficient technique to get three-dimensional seal and prevent microbial contamination of canals in the endodontic therapy.


Keywords


endodoncia; Enterococcus faecalis; gutapercha; microbiología oral; microfiltración; obturación de conductos; técnica condensación híbrida mixta

Full Text:

PDF (Español)

References


Tomson RM, Polycarpou N, Tomson PL. Contemporary obturation of the root canal system. Br Dent J. 2014 Mar; 216(6): 315-22.

Muñoz ID. Microfiltración apical en dos técnicas de obturación: Condensación Lateral y el Sistema Obtura II. Rev Nal Odo UCC. 2009 Ene-Jun; 5(8): 21-9.

Aptekar A, Ginnan K. Comparative analysis of microleakage and seal for 2 obturation materials: Resilon/epiphany and gutta-percha. J Can Dent. 2006 Apr; 72(3): 245.

Guzmán B, Koury JM, García E, Méndez C, Antúnez M. Interfase TopSeal-dentina en relación con dos técnicas de obturación: condensación lateral y técnica termoplastificada/termorreblandecida. Estudio de microscopía electrónica de barrido. Univ Odontol. 2010 Ene-Jun; 29(62): 39-44.

Gilhooly RM, Hayes SJ, Bryant ST, Dummer PM. Comparison of cold lateral condensation and a warm multiphase gutta-percha technique for obturating curved root canals. Int Endod J. 2000 Sep; 33(5): 415-20.

Cardoso A, Kenji C, Castro L. Single-cone obturation technique: a literatura Review. RSBO. 2012 Jun; 9(4): 442-47.

Gençoğlu N, Garip Y, Baş M, Samani S. Comparison of different gutta-percha root filling techniques: Thermafil, Quick-Fill, System B, and lateral condensation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002 Mar; 93(3): 333-36.

Tennert C, Jungbäck IL, Wrbas KT. Comparison between two thermoplastic root canal obturation techniques regarding extrusion of root canal filling—a retrospective in vivo study. Clin Oral Invest. 2013 Mar; 17(2): 449-54.

Gutmann J, Saunders W, Nguyen L. An assessment of the plastic Thermafil obturation Technique Part I Radiographic evaluation of adaptation and placement. Int Endod J. 1993 May; 26(3): 173-8.

Schneider S. A comparison of canal preparations in straight and curved root canals. Oral Surg. 1971 Aug; 2(32): 271-5.

Sjogren U, Hagglund B, Sundqvist G, Wing K. Factors affecting the long-term results of endodontic treatment. J Endod. 1990 Oct; 16(10): 498-504

Lin LM, Skribner JE, Gaengler P. Factors associated with endodontic treatment failures. J Endod. 1992 Dec; 18(12): 625-27.

Nabeshima CK, Martins GH, De Pasquali MF, Furukava RC, Cai S, De Lima ME. Comparison of three obturation techniques with regard to bacterial leakage. Braz J Oral Sci. 2013 Jul-Sep; 12(3): 212-15.

Lea CS, Apicella MJ, Mines P, Yancich PP, Parker MH. Comparison of the obturation density of cold lateral compaction versus warm vertical compaction using the continuous wave of condensation technique. J Endod. 2005 Jan; 31(1): 37-9.

Robberecht L, Colard T, Claisse-Crinquette A. Qualitative evaluation of two endodontic obturation techniques: tapered single-cone method versus warm vertical condensation and injection System. An in vitro study. J Oral Sci. 2012 Mar; 54(1): 99-104.

Pontarollo G, Hamerschmitt R, Coelho B, Leonardi DP, Fagundes FS. Bacterial infiltration comparison of two root canal filling techniques. RSBO. 2014 Apr-Jun; 11(2): 166-71.

Wu M, van der Sluis LW, Wesselink PR. A preliminary study of the percentage of gutta-percha-filled area in the apical canal filled with vertically compacted warm gutta-percha. Int Endod J. 2002 Jun; 35(6): 527-35.

Marciano MA, Ordinola-Zapata R, Cunha TV, Duarte MA, Cavenago BC, Garcia RB, Bramante CM, Bernardineli N, et al. Analysis of four gutta-percha techniques used to fill mesial root canals of mandibular molars. Int Endod J. 2011 Apr; 44(4): 321-29.

Schäfer E, Nelius B, Bürklein S. A comparative evaluation of gutta-percha filled areas in curved root canals obturated with different techniques. Clin Oral Invest. 2012 Feb; 16(1): 225-30.

Reddy ES, Sainath D, Nerenderreddy M, Pasari S, Vallikanthan S, Sindhurareddy G. Cleaning efficiency of anatomic endodontic technology, ProFile System and manual instrumentation in oval-shaped root canals: An in vitro study. J Contemp Dent Pract. 2013 Jul-Aug; 14(4): 629-34.

Hegde V, Arora S. Sealing ability of a novel hydrophilic vs conventional hydrophobic obturation systems: A bacterial leakage study. J Conserv Dent. 2015 Jan-Feb; 18(1): 62-5.

Moeller L, Wenzel A, Wegge-Larsen, AM, Ding M, Kirkevang LL. Quality of root fillings performed with two root filling techniques. An in vitro study using micro-CT. Acta Odontol Scand. 2013 May-jul; 71(3-4): 689-96.

Cohen BD, Combe EC, Lilley JD. Effect of thermal placement techniques on some physical properties of gutta-percha. Int Endod J. 1992 Nov; 25(6): 292-96.

Tanomaru-Filho M, Silveira GF, Tanomaru JM, Bier CA. Evaluation of the thermoplasticity of different gutta-percha cones and Resilon®. Aust Endod J. 2007 Apr; 33(1): 23-6.

Diemer F, Sinan A, Calas P. Penetration depth of warm vertical Gutta-Percha pluggers: impact of apical preparation. J Endod. 2006; 32(2): 123-26.

Keleş A, Alcin H, Kamalak A, Versiani MA. Micro-CT evaluation of root filling quality in oval-shaped canals. Int Endod J. 2014 Dec; 47(12): 1177-84.

De-Deus G, Murad C, Paciornik S, Reis CM, Coutin-Filho T. The effect of the canal-filled area on the bacterial leakage of oval-shaped canals. Int Endod J. 2008 Mar; 41(3): 183-90.




DOI: http://dx.doi.org/10.11144/Javeriana.uo35-74.emef

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM




Copyright (c) 2016 Silvia Elena Aragón Matamoros, Tomás Alberto Guindos Moya, Yanina Meza González, Diana Morales Herrera, María Alejandra Perera Díaz, Adriana Rodríguez Ciódaro, Hugo Diez Ortega, Catalina Méndez De la Espriella

Creative Commons
This work is registered under Creative Commons Attribution 4.0 International.
Created from http://revistas.javeriana.edu.co/.