Brain tissue preservation and vascular staining techniques: A literature review
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Jan 18, 2021
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Maria Isabel Ocampo Navia
https://orcid.org/0000-0003-1148-7523
Juan Carlos Gomez-Vega
https://orcid.org/0000-0002-4385-5063
https://orcid.org/0000-0003-1148-7523
Juan Carlos Gomez-Vega
https://orcid.org/0000-0002-4385-5063
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Abstract
A precise understanding of cerebral anatomy is fundamental in the practice and training of neurosurgeons. The anatomic study of cadaveric specimens has become one of the most useful methods of education in this field, since it facilitates the study of anatomic variations in different populations and refines critical surgical skills such as technique, depth, and visual spatiality through dissections for a better understanding of the human anatomy and the central nervous system. Thus, brain tissue preservation and coloration techniques have emerged as alternatives to standard morphological education, training and learning for neurosurgery residents. This article pretends to describe this techniques and the different cerebral vascular coloration methods. We seek to facilitate the creation of microsurgery laboratories and propose new alternatives that can be applied in the education of students and neurosurgery residents.
Keywords
brain, cadaver, dissection, neuroanatomy, plastinationcerebro, cadáver, neuroanatomía, disección, plastinación
References
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2. Sanan A, Aziz K, Janjua R, Loveren H, Keller J. Colored Silicone Injection for Use in Neurosurgical Dissections : Anatomic Technical Note. 1999;45(5).
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9. Breve U, Plastinaci D. A Brief Review on the History , Methods and Applications of Plastination. 2010;28(4):1075–9.
10. Elnady FA. The Elnady Technique : An Innovative , New Method for Tissue Preservation. 2016;33(3):237–42.
11. Ravi SB, Bhat VM. Plastination : A novel , innovative teaching adjunct in oral pathology. 2018;15(2):133–7.
12. Bickley HC, Conner RS, Walker AN, Jackson RL. Harmon C. Bickley, Robert S.Conner Anna N. Walker and R. Lamar Jackson. :30–9.
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16. Díaz M, Suárez C, Rodríguez S, Valbuena A, Cruz C, Barahona S, et al. Artículo de reflexión: comparación de técnicas de conservación morfológica y su posible aplicación para la enseñanza de la anatomía. 2014;6(3).
17. Triviño Casado, A.; Ramírez Sebastián, J.M.; García Sánchez J. Método combinado de diafanización y relleno vascular para el estudio de la vascularización del globo ocular. Arch Soc Esp Oftalmol. 1980;
18. Scala G. Microvasculature of the Cerebral Cortex : A Vascular Corrosion Cast and Immunocytochemical Study. 2014;263(September 2013):257–63.
19. Limpastan K, Vaniyapong T, Watcharasaksilp W, Norasetthada T. Silicone injected cadaveric head for neurosurgical dissection : Prepared from defrosted cadaver. 2013;8(2):10–2.
20. Inoue T, Kobayashi S, Sugita K. Dye Injection Method for the Demonstration of Territories Supplied by Individual Perforating Arteries of the Posterior Communicating Artery in the Dog. :684–7.
21. Riepertinger A. E 20 color-injection. 1989;8–12.
22. Kaya AH, Sam B, Celik F. A quick-solidifying , coloured silicone mixture for injecting into brains for autopsy : technical report. 2006;i:322–6.
23. Urgun K, Toktas ZO, Akakin A, Yilmaz B, Şahin S, Kiliç T. A Very Quickly Prepared , Colored Silicone Material for Injecting into Cerebral Vasculature for Anatomical Dissection : A Novel and Suitable Material for both Fresh and Non-Fresh Cadavers. 2016;1–6.
24. Shkarubo MA, Shkarubo AN, Dobrovolsky GF, Polev GA, Chernov I V, Andreev DN, et al. Making Anatomical Preparations of the Human Brain Using Colored Silicone for vascular Perfusion Staining (technical description). World Neurosurg [Internet]. 2018; Available from: https://doi.org/10.1016/j.wneu.2018.01.102
25. Blaney SP, Johnson B. Technique for reconstituting fixed cadaveric tissue. Anat Rec. 1989 Aug;224(4):550–1.
26. Krishnamurthy S, Powers SK. The use of fabric softener in neurosurgical prosections. Neurosurgery. 1995 Feb;36(2):420–4.
27. Macdonald GJ, MacGregor DB. Procedures for embalming cadavers for the dissecting laboratory. Proc Soc Exp Biol Med. 1997 Sep;215(4):363–5.
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29. Martins C. Rhoton Papers. 2016;1–14.
30. Latarjet M, Juttin P. [Use of injections of plastic substances in anatomopathological studies of the lung]. Poumon. 1952 May;8(5):459–63.
31. Latarjet M. Plastics in anatomical technique. Sem Med. 1952 Oct;28(74):10.
2. Sanan A, Aziz K, Janjua R, Loveren H, Keller J. Colored Silicone Injection for Use in Neurosurgical Dissections : Anatomic Technical Note. 1999;45(5).
3. Benet A, Rincon-Torroella J, Lawton MT, Gonzalez Sánchez J. Novel embalming solution for neurosurgical simulation in cadavers. 2014;120(May):1229–37.
4. Alvernia J, Pradilla G, Mertens P, Tamargo R. Surgical Anatomy and Technique Latex Injection of Cadaver Heads : Technical Note. 2010;67(December):362–7.
5. Mehra S, Houdhary RC, Uli AT. Dry Preservation of Cadaveric Hearts : An Innovative Trial. 2003;36:34–6.
6. Pineda EP, Alfonso J, Guerra B, Luque RM. Revisión: La plastinación como técnica de preservación de material biológico para docencia e investigación en anatomía. 2017;9(1):55–62.
7. Slater D. Health Hazards of formaldehyde. Vol. 1, Lancet (London, England). England; 1981. p. 1099.
8. Bhandari K, Acharya S, Srivastava AK, Kumari R, Nimmagada K. Plastination: a new model of teaching anatomy. 2016;4(3):3–6.
9. Breve U, Plastinaci D. A Brief Review on the History , Methods and Applications of Plastination. 2010;28(4):1075–9.
10. Elnady FA. The Elnady Technique : An Innovative , New Method for Tissue Preservation. 2016;33(3):237–42.
11. Ravi SB, Bhat VM. Plastination : A novel , innovative teaching adjunct in oral pathology. 2018;15(2):133–7.
12. Bickley HC, Conner RS, Walker AN, Jackson RL. Harmon C. Bickley, Robert S.Conner Anna N. Walker and R. Lamar Jackson. :30–9.
13. Venegas C, Dalmau E, Trujillo C, Díaz C. La técnica de plastinación por corrosión : realidad posible. 2013;
14. Cook P, Dawson B. Plastination methods. 1995;
15. Rodriguez F, Algarilla D. Diafanización : Técnica Modificada por Solución Rojo.
16. Díaz M, Suárez C, Rodríguez S, Valbuena A, Cruz C, Barahona S, et al. Artículo de reflexión: comparación de técnicas de conservación morfológica y su posible aplicación para la enseñanza de la anatomía. 2014;6(3).
17. Triviño Casado, A.; Ramírez Sebastián, J.M.; García Sánchez J. Método combinado de diafanización y relleno vascular para el estudio de la vascularización del globo ocular. Arch Soc Esp Oftalmol. 1980;
18. Scala G. Microvasculature of the Cerebral Cortex : A Vascular Corrosion Cast and Immunocytochemical Study. 2014;263(September 2013):257–63.
19. Limpastan K, Vaniyapong T, Watcharasaksilp W, Norasetthada T. Silicone injected cadaveric head for neurosurgical dissection : Prepared from defrosted cadaver. 2013;8(2):10–2.
20. Inoue T, Kobayashi S, Sugita K. Dye Injection Method for the Demonstration of Territories Supplied by Individual Perforating Arteries of the Posterior Communicating Artery in the Dog. :684–7.
21. Riepertinger A. E 20 color-injection. 1989;8–12.
22. Kaya AH, Sam B, Celik F. A quick-solidifying , coloured silicone mixture for injecting into brains for autopsy : technical report. 2006;i:322–6.
23. Urgun K, Toktas ZO, Akakin A, Yilmaz B, Şahin S, Kiliç T. A Very Quickly Prepared , Colored Silicone Material for Injecting into Cerebral Vasculature for Anatomical Dissection : A Novel and Suitable Material for both Fresh and Non-Fresh Cadavers. 2016;1–6.
24. Shkarubo MA, Shkarubo AN, Dobrovolsky GF, Polev GA, Chernov I V, Andreev DN, et al. Making Anatomical Preparations of the Human Brain Using Colored Silicone for vascular Perfusion Staining (technical description). World Neurosurg [Internet]. 2018; Available from: https://doi.org/10.1016/j.wneu.2018.01.102
25. Blaney SP, Johnson B. Technique for reconstituting fixed cadaveric tissue. Anat Rec. 1989 Aug;224(4):550–1.
26. Krishnamurthy S, Powers SK. The use of fabric softener in neurosurgical prosections. Neurosurgery. 1995 Feb;36(2):420–4.
27. Macdonald GJ, MacGregor DB. Procedures for embalming cadavers for the dissecting laboratory. Proc Soc Exp Biol Med. 1997 Sep;215(4):363–5.
28. Goodrich JT. A millennium review of skull base surgery. Childs Nerv Syst. 2000 Nov;16(10–11):669–85.
29. Martins C. Rhoton Papers. 2016;1–14.
30. Latarjet M, Juttin P. [Use of injections of plastic substances in anatomopathological studies of the lung]. Poumon. 1952 May;8(5):459–63.
31. Latarjet M. Plastics in anatomical technique. Sem Med. 1952 Oct;28(74):10.
How to Cite
Ocampo Navia, M. I., & Gomez-Vega, J. C. (2021). Brain tissue preservation and vascular staining techniques: A literature review. Universitas Medica, 62(1). https://doi.org/10.11144/Javeriana.umed62-1.teac
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