The Effect of Capsaicine in Human Pulp Fibroblasts, in the Production of PGE2 and Proinflammatory Cytokines...
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Jun 28, 2016
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Abstract
ABSTRACT. Background: due of capsaicin effect on the control of various inflammatory mediators, it has been proposed to modulate inflammatory processes caused by physical assaults to pulp tissue. Purpose: to evaluate the effect of capsaicin diluted in 0.05 % ethanol and its vehicle on PGE2 production, IL-8, IL-6, IL-1β, and IL-12p70 in Human Fibroblasts Pulp (FPH). Methods: concentrations of PGE2, IL-8, IL-6, IL-1β, and IL-12p70 were analyzed by ELISA or flow cytometry in supernatants of FPH at 6 and 8 hours, after being stimulated with capsaicin 5*10-5 M and 2,5*10-5 M and ethanol 0.05% vehicle of capsaicin and compared with unstimulated cells. Results: ethanol 0.05% as a diluent capsaicin, is responsible for the effect on the decrease in the expression of PGE2, IL-8 and IL-6 at the times and concentrations evaluated; and IL-12p-70 was the only cytokine that increased significantly in the presence of capsaicin 2,5*10-5 M at 8h, it is statistically greater than ethanol 0.05%. Capsaicin and ethanol had not significant effect on IL-1β. Conclusion: the effect of capsaicin on FPH, is associated with ethanol 0.05% used as diluent and varies depending on time and the mediator analyzed.
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References
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15. Bhutani M, Pathak AK, Nair AS, Kunnumakkara AB, Guha S, Sethi G, Aggarwal BB. Capsaicin is a novel blocker of constitutive and interleukin-6-inducible STAT3 activation. Clin Cancer Res. 2007 May 15; 13(10): 3024-32.
16. Huang J, Qiu L, Ding L, Wang S, Wang J, Zhu Q, Song F, Hu J. Ginsenoside Rb1 and paeoniflorin inhibit transient receptor potential vanilloid-1-activated IL-8 and PGE2 production in a human keratinocyte cell line HaCaT. Int Immunopharmacol. 2010 Oct; 10(10): 1279-83. DOI: 10.1016/j.intimp.2010.07.010
17. Holzer P. Capsaicin: cellular targets, mechanisms of action, and selectivity for thin sensory neurons. Pharmacol Rev. 1991 Jun; 43(2): 143-201.
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21. Lee IO, Lee KH, Pyo JH, Kim JH, Choi YJ, Lee YC. Anti-inflammatory effect of capsaicin in Helicobacter pylori-infected gastric epithelial cells. Helicobacter. 2007 Oct; 12(5): 510-7. DOI: 10.1111/j.1523-5378.2007.00521.x
22. Reilly CA, Taylor JL, Lanza DL, Carr BA, Crouch DJ, Yost GS. Capsaicinoids cause inflammation and epithelial cell death through activation of vanilloid receptors. Toxicol Sci. 2003 May; 73(1): 170-81. DOI: 10.1093/toxsci/kfg044
23. Van der Pouw Kraan TC, Boeije LC, Smeenk RJ, Wijdenes J, Aarden LA. Prostaglandin-E2 is a potent inhibitor of human interleukin 12 production. J Exp Med. 1995 Feb 1; 181(2): 775-9
24. Mitsuhashi M, Liu J, Cao S, Shi X, Ma X.Regulation of interleukin-12 gene expression and its anti-tumor activities by prostaglandin E2 derived from mammary carcinomas. J Leukoc Biol. 2004 Aug; 76(2): 322-32. DOI: 10.1189/jlb.120364125.
25. Reuter S, Charlet J, Juncker T, Teiten MH, Dicato M, Diederich M. Effect of curcumin on nuclear factor kappaB signaling pathways in human chronic myelogenous K562 leukemia cells. Ann N Y Acad. 2009 Aug; 1171: 436-47. DOI: 10.1111/j.1749-6632.2009.04731.x
26. Singh S, Natarajan K, Aggarwal BB. Capsaicin (8-methyl-N-vanillyl-6-nonenamide) is a potent inhibitor of nuclear transcription factor-kappa B activation by diverse agents. J Immunol. 1996 Nov; 157(10): 4412-20.
27. Kang JH, Kim CS, Han IS, Kawada T, Yu R. Capsaicin, a spicy component of hot peppers, modulates adipokine gene expression and protein release from obese-mouse adipose tissues and isolated adipocytes, and suppresses the inflammatory responses of adipose tissue macrophages. FEBS Lett. 2007 Sep18; 581(23): 4389-96. DOI: 10.1016/j.febslet.2007.07.082
28. Seki N, Shirasaki H, Kikuchi M, Himi T. Capsaicin induces the production of IL-6 in human upper respiratory epithelial cells. Life Sci. 2007 Apr; 80(17): 1592-7. DOI: 10.1016/j.lfs.2007.01.037
2. Cohen JS, Reader A, Fertel R, Beck M, Meyers WJ. A radioimmunoassay determination of the concentrations of prostaglandins E2 and F2alpha in painful and asymptomatic human dental pulps. J Endod.1985 Aug; 11(8): 330-5.
3. Barkhordar RA, Hayashi C, Hussain MZ. Detection of interleukin-6 in human dental pulp and periapical lesions. Endod Dent Traumatol. 1999 Feb; 15(1): 26-7.
4. Buck SH, Burks TF. The neuropharmacology of capsaicin: review of some recent observations. Pharmacol Rev. 1986 Sep; 38(3): 179-226.
5. Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D. The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature. 1997 Oct 23; 389(6653): 816-24.
6. Denda M, Fuziwara S, Inoue K, Denda S, Akamatsu H, Tomitaka A, et al. Immunoreactivity of VR1 on epidermal keratinocyte of human skin. Biochem Biophys Res Com. 2001 Aug 3;285(5): 1250-2. DOI: 10.1006/bbrc.2001.5299
7. Dvorakova M, Kummer W. Transient expression of vanilloid receptor subtype 1 in rat cardiomyocytes during development. Histochem Cell Biol 2001 Sep; 116(3): 223-5. DOI: 10.1007/s004180100308
8. Partsch G, Matucci-Cerinic M, Marabini S, Jantsch S, Pignone A, Cagnoni M. Collagenase synthesis of rheumatoid arthritis synoviocytes: dose-dependent stimulation by substance P and capsaicin. Scand J Rheumatol. 1991; 20(2): 98-103.
9. Matucci-Cerinic M, Marabini S, Jantsch S, Cagnoni M, Partsch G. Effects of capsaicin on the metabolism of rheumatoid arthritis synoviocytes in vitro. Ann Rheum Dis. 1990 Aug; 49(8): 598-602.
10. Southall MD, Li T, Gharibova LS, Pei Y, Nicol GD, Travers JB. Activation of epidermal vanilloid receptor-1 induces release of proinflammatory mediators in human keratinocytes. J Pharmacol Exp Ther. 2003 Jan; 304(1): 217-22. DOI: 10.1124/jpet.102.040675
11. Veronesi B, Carter JD, Devlin RB, Simon SA, Oortgiesen M. Neuropeptides and capsaicin stimulate the release of inflammatory cytokines in a human bronchial epithelial cell line. Neuropeptides. 1999 Dec; 33(6): 447-56. DOI: 10.1054/npep.1999.0761
12. Miyamoto R, Tokuda M, Sakuta T, Nagaoka S, Torii M. Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures. J Endod. 2005 Sep; 31(9): 652-8.
13. Moreno GC, González JM, Caviedes JF. Efecto de la capsaicina sobre la proliferacion y ciclo celular de fibroblastos pulpares humanos. Federación Odontológica Colombiana. 2002: 89-99.
14. Hautkappe M, Roizen MF, Toledano A, Roth S, Jeffries JA, Ostermeier AM. Review of the effectiveness of capsaicin for painful cutaneous disorders and neural dysfunction. Clin J Pain. 1998 Jun; 14(2): 97-106.
15. Bhutani M, Pathak AK, Nair AS, Kunnumakkara AB, Guha S, Sethi G, Aggarwal BB. Capsaicin is a novel blocker of constitutive and interleukin-6-inducible STAT3 activation. Clin Cancer Res. 2007 May 15; 13(10): 3024-32.
16. Huang J, Qiu L, Ding L, Wang S, Wang J, Zhu Q, Song F, Hu J. Ginsenoside Rb1 and paeoniflorin inhibit transient receptor potential vanilloid-1-activated IL-8 and PGE2 production in a human keratinocyte cell line HaCaT. Int Immunopharmacol. 2010 Oct; 10(10): 1279-83. DOI: 10.1016/j.intimp.2010.07.010
17. Holzer P. Capsaicin: cellular targets, mechanisms of action, and selectivity for thin sensory neurons. Pharmacol Rev. 1991 Jun; 43(2): 143-201.
18. Trevisani M, Smart D, Gunthorpe MJ, Tognetto M, Barbieri M, Campi B, Amadesi S, Gray J, Jerman JC, Brough SJ, Owen D, Smith GD, Randall AD,Harrison S, Bianchi A, Davis JB, Geppetti P. Ethanol elicits and potentiates nociceptor responses via the vanilloid receptor-1. Nat Neurosci. 2002 Jun; 5(6): 546-51. DOI: 10.1038/nn852
19. Vetter I, Wyse BD, Roberts-Thomson SJ, Monteith GR, Cabot PJ. Mechanisms involved in potentiation of transient receptor potential vanilloid 1 responses by ethanol. Eur J Pain. 2008 May; 12(4): 441-54. DOI: 10.1016/j.ejpain.2007.07.001
20. Gazzieri D, Trevisani M, Springer J, Harrison S, Cottrell GS, Andre E, Nicoletti P, Massi D, Zecchi S, Nosi D, Santucci M, Gerard NP, Lucattelli M, Lungarella G, Fischer A, Grady EF, Bunnett NW, Geppetti P. Substance P released by TRPV1-expressing neurons produces reactive oxygen species that mediate ethanol-induced gastric injury. Free Radic Biol Med. 2007 Aug; 43(4): 581-9. DOI: 10.1016/j.freeradbiomed.2007.05.018
21. Lee IO, Lee KH, Pyo JH, Kim JH, Choi YJ, Lee YC. Anti-inflammatory effect of capsaicin in Helicobacter pylori-infected gastric epithelial cells. Helicobacter. 2007 Oct; 12(5): 510-7. DOI: 10.1111/j.1523-5378.2007.00521.x
22. Reilly CA, Taylor JL, Lanza DL, Carr BA, Crouch DJ, Yost GS. Capsaicinoids cause inflammation and epithelial cell death through activation of vanilloid receptors. Toxicol Sci. 2003 May; 73(1): 170-81. DOI: 10.1093/toxsci/kfg044
23. Van der Pouw Kraan TC, Boeije LC, Smeenk RJ, Wijdenes J, Aarden LA. Prostaglandin-E2 is a potent inhibitor of human interleukin 12 production. J Exp Med. 1995 Feb 1; 181(2): 775-9
24. Mitsuhashi M, Liu J, Cao S, Shi X, Ma X.Regulation of interleukin-12 gene expression and its anti-tumor activities by prostaglandin E2 derived from mammary carcinomas. J Leukoc Biol. 2004 Aug; 76(2): 322-32. DOI: 10.1189/jlb.120364125.
25. Reuter S, Charlet J, Juncker T, Teiten MH, Dicato M, Diederich M. Effect of curcumin on nuclear factor kappaB signaling pathways in human chronic myelogenous K562 leukemia cells. Ann N Y Acad. 2009 Aug; 1171: 436-47. DOI: 10.1111/j.1749-6632.2009.04731.x
26. Singh S, Natarajan K, Aggarwal BB. Capsaicin (8-methyl-N-vanillyl-6-nonenamide) is a potent inhibitor of nuclear transcription factor-kappa B activation by diverse agents. J Immunol. 1996 Nov; 157(10): 4412-20.
27. Kang JH, Kim CS, Han IS, Kawada T, Yu R. Capsaicin, a spicy component of hot peppers, modulates adipokine gene expression and protein release from obese-mouse adipose tissues and isolated adipocytes, and suppresses the inflammatory responses of adipose tissue macrophages. FEBS Lett. 2007 Sep18; 581(23): 4389-96. DOI: 10.1016/j.febslet.2007.07.082
28. Seki N, Shirasaki H, Kikuchi M, Himi T. Capsaicin induces the production of IL-6 in human upper respiratory epithelial cells. Life Sci. 2007 Apr; 80(17): 1592-7. DOI: 10.1016/j.lfs.2007.01.037
How to Cite
Bedoya Mejía, M. A., Rodríguez Camacho, L. S., Jaramillo Gómez, L. M., & Moreno Abello, G. C. (2016). The Effect of Capsaicine in Human Pulp Fibroblasts, in the Production of PGE2 and Proinflammatory Cytokines. Universitas Odontologica, 35(74), 75–83. https://doi.org/10.11144/Javeriana.uo35-74.ecfp
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Basic Sciences, Biotechnology and Bioinformatics