Characterization of the expression of ß2-Glycoprotein l in human umbilical cord vein endothelial cells
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Background: Antiphospholipid syndrome (APS) is an autoimmune disease characterized by thrombosis and gestational disorders in the presence of antiphospholipid antibodies (aPL). ß2-Glycoprotein l (ß2-GPl) is one of the main antigens recognized by aPL. It is not clear if the basal expression of ß2-GPl is different between different pregnant women, nor if this event could explain the worse clinical outcomes in some patients with alterations such as APS.
Objective: Characterize the expression of ß2-GPl in different clones and cultures passages of human umbilical cord vein endothelial cells (HUVEC) of healthy pregnant women.
Methods: We isolated HUVEC cells by enzymatic and mechanical digestion with type l collagenase and subsequent, we detected CD31 and ß2-GPl expression by flow cytometry and fluorescence microscopy.
Results: ß2-GPl expression varies between HUVEC cells of different clones of healthy pregnant women. For clone one the positivity was 6.25%, while for clones 2 and 3, it was 81.9 and 88.9%, respectively.
Conclusions: The expression of ß2-GPl in endothelial cells could vary between healthy pregnant women. Therefore, in vitro models for the study of endothelial dysfunction in APS needs to add this cofactor to the culture.
antiphospholipid syndrome, antibodies, antiphospholipid, endothelial cells, thrombosissíndrome antifosfolípido, anticuerpos antifosfolípidos, células endoteliales, trombosis
2. Velásquez M, Rojas M, Abrahams VM, et al. Mechanisms of Endothelial Dysfunction in Antiphospholipid Syndrome: Association With Clinical Manifestations. Front Physiol. 2018-9.
3. Velásquez M, Granada MA, Galvis JC, et al. Estrés oxidativo en células endoteliales inducido por el suero de mujeres con diferentes manifestaciones clínicas del síndrome antifosfolípido. Biomédica. 2019;39(4).
4. Beltagy A, Trespidi L, Gerosa M, et al. Antiphospholipid antibodies and reproductive failures. Am J Reprod Immunol, 2020; e13258.