Use of modified microarrays (tissue microarrays) for the study of placental samples
https://orcid.org/0000-0003-4042-8864
Yhoiss Smiht Muñoz Cerón
https://orcid.org/0000-0002-8464-8941
Lina Magali Paredes León
https://orcid.org/0000-0001-8247-7117
Liliana Salazar Monsalve
https://orcid.org/0000-0002-3087-8493
María Carolina Pustovrh
https://orcid.org/0000-0002-2909-1941
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Introduction: The placenta is an essential source of information for medical research. Therefore, it is necessary to implement techniques that save tissue usage, optimize processing time, and reduce operating costs.
Objective: Introduce a protocol for the placental samples study based on the tissue microarrays technique.
Materials and methods: The fragments were obtained from four quadrants of human placentas at term and fixed in 4% paraformaldehyde. Each fragment was dehydrated and infiltrated with kerosene. To perform the microarray sections of each quadrant's villous zone were obtained with 4mm (5/32") round punch. Each sample was placed in a single metal mold with liquid kerosene. From the block obtained, 4 µm thick sections were made and stained with hematoxylin-eosin.
Conclusions: The application of this technique is useful for placental samples. It can be used to obtain blocks from different placenta regions to apply histochemical and immunohistochemical or molecular techniques. Advantage: Performing simultaneous and standardized analysis of multiple samples with a sufficient area to be analyzed.
placenta; tissue microarrays; techniqueplacenta; tissue microarrays; técnica
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