Palavras-chave
rata
preñez
morfometría
Como Citar
Resumo
Introducción: El correcto funcionamiento de la placenta garantiza un estado nutricional fetal adecuado.
Objetivo: Caracterizar histomorfológicamente la placenta temprana de biomodelos alimentados con dieta de cafetería.
Materiales y métodos: Estudio experimental. 18 ratas Wistar fueron distribuidas en grupo control (CTRL=10) y en grupo experimental (CAF=8). CTRL alimentado con dieta estándar y CAF con dieta de cafetería. Al día 12.5 de gestación, se extrajeron 18 placentas, se procesaron histoquímicamente, se fotografiaron y midieron usando el microscopio Leica DM750, y el software ImageProPlus 7.0. Variables evaluadas: grosor central, área total, grosor y área del laberinto placentario (ZL).
Resultados: Peso unidad feto-placentaria: CAF: 0.2368 ± 0.0068 g; CTRL: 0.2622 ±0.0096 g; grosor central: CAF: 1727± 151 µm; CTRL: 1936± 140 µm; área placentaria total: CAF: 8.130 x 106 ± 0.511 x 106 µm2; CTRL: 9.493 x 106 ± 0.587 x 106 µm2; grosor ZL: CAF: 409± 56 µm; CTRL: 492 ±26 µm; área ZL: CAF: 1.240 x 106 ± 0.253 x 106 µm2; CTRL: 1.329 x 106 ± 0.165 x 106 µm2). No se encontraron diferencias significativas.
Conclusiones: No se evidenció afectación de la morfometría placentaria. Se recomienda estudiar otras variables como grado de vascularización y transportadores moleculares específicos.
Conflicto de interés: Ninguno
1. de Barros Mucci D, Kusinski LC, Wilsmore P, Loche E, Pantaleão LC, Ashmore TJ, et al. Impact of maternal obesity on placental transcriptome and morphology associated with fetal growth restriction in mice. Vol. 44, International Journal of Obesity. 2020. p. 1087–96.
2. Lin YJ, Huang LT, Tsai CC, Sheen JM, Tiao MM, Yu HR, et al. Maternal high-fat diet sex-specifically alters placental morphology and transcriptome in rats: Assessment by next-generation sequencing. Placenta. 2019;78(March):44–53.
3. Song L, Sun B, Boersma GJ, Cordner ZA, Yan J, Moran TH, et al. Prenatal high-fat diet alters placental morphology, nutrient transporter expression, and mtorc1 signaling in rat. Obesity. 2017; 25:909–19.
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